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Suspensions of field samples suspected to contain FMD virus should be clarified and inoculated into cell cultures or unweaned mice.

将可疑含FMD病毒的田间样品酊悬浮液经澄清后接种于细胞培养物或未断奶乳鼠。

Suspensions of field samples suspected to contain FMD virus should be clarified and inoculate d into cell cultures or unweaned mice.

将可疑含FMD病毒的田间样品酊悬浮液经澄清后接种于细胞培养物或未断奶乳鼠。

Suspensions of field samples suspect ed to contain FMD virus should be clarified and inoculated into cell cultures or unweaned mice.

将可疑含FMD病毒的田间样品酊悬浮液经澄清后接种于细胞培养物或未断奶乳鼠。

When inoculating, the modalities ofcells maybe round, triangle or virgulate; and began to be adhesive for 48h; andformation of colony could be formed on day 4-5; Many cells grew in clonalmanner on day 7, and the cultured cells were characterized by large spindle-shapedappearance by the time of 10 days.

刚接种时细胞形态呈圆形、三角形或棒杆状,48h 后细胞开始贴壁,4-5d 可见有集落形成,第7d 形成多个细胞克隆,第10d 细胞呈长梭形,2w 左右可达到基本融合;传代细胞呈均匀的纺锤形,基本上无悬浮细胞。

The production and characterization of endoglucanase from Streptomyces xylophagus KX6 was studied. Maximum endoglucanase yield of 0.538 IU/ml was achieved with medium pH8.0, containing CMC2Na 1.0% as carbon resource, soybean meal 1% as nitrogen resource, 2% inoculating volume, 30% 250 ml triangle flask bulk for medium volume at 40℃ 200r/min shaker for 48h.

实验中对其产酶的液态发酵条件进行了研究,碳源为1%羧甲基纤维素钠,氮源为1%豆粕粉,250ml三角瓶30 %装液量,接种量为2%,培养基初始pH为8.0,培养温度为40℃,200r/min培养48h后,发酵液中内切葡聚糖酶活达到0.538IU/ml。

Results The results showed that pigs in the groups given higher immunizing dose were developed higher levels HI antibody.

以不同的剂量分别经肌肉注射免疫后, HI抗体水平与接种剂量呈正相关。

Using corn starch as main material,after liquefaction and saccharification ,several kinds of saccharified liquid were prepared and fermented by different lactobacillus.

以玉米淀粉为主要原料,经液化、糖化后获得糖化液进行调配,然后分别接种不同乳酸菌进行发酵,通过正交试验等实验方法进行优化,确定出最佳配方和工艺条件,从而制成具有较高品质的产品。

After 72 hours incubation, the chondrocytes were digested into single cell suspension. Then they were incubated in the 96-well culture plate with the density of 1×10^8 L^(-1), 100 μL per well and six wells for per group.

白细胞介素1β加入量为5μg/L,培养72h将实验用细胞消化传代后制成单细胞悬液,以1×10^8L^的密度接种于96孔培养板,100μL/孔,每组6孔。

The PMTE and PG enzymes activity peaks with the ginsengs pathogenesis appeared in same time. The results demonstrated that PMTE and PG have main function in C.

人参抗锈腐病主要防御酶的研究:三年生人参和一年生人参接种锈腐菌后:SOD、CAT、POD、PPO、PAL 5种酶活性均升高。

After inoculation of leukemia virus,the changes in ANAE,AcP and β-Gase reactions were observed in the thymocyte and thymie nurse cell in thymus cortex of experimental mice.

对接种白血病病毒后30d、75d 小鼠胸腺和淋巴结的组织结构及酸性非特异性酯酶、酸性磷酸酶、β~葡糖苷酸酶反应类型进行了观察,并与正常组作对照。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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