接种后的

Results showed endothelialcells inoculated by shimen virulent strain of CSFV have no obvious change after 12h,24h;At36h,cells become be elongate and appearanced defluvium;At 48h,a few of cells started tobecome round and intercellular space become auxesis;At 72h, intercellular space becomemore auxesis, defluvium of cells was acute.

结果表明，血管内皮细胞于接种 CSFV 石门株后 12h，24h 无明显变化，至 36h 时，细胞开始拉长，个别细胞开始脱落；至 48h 时，部分细胞变圆，脱落，细胞间隙增大；至 72h，细胞拉长呈长梭状，细胞间隙很大，细胞脱落严重。3 个对照组的细胞均未出现明显变化。

One-day-old SPF chicklings were inoculated via intracephalic injection at a dose of 0.03ml per chickling, consisting of 1:100 tissuer virus suspension. And 4 of experimental group and 2 of control group were killed at 1, 3, 5, 10, 14, 20, 25 and 30 days postinfection, respectively. All the sera, the lymphocyte suspension of peripheral blood, thymus, Fabricius bursa and spleen, were collected.

本研究用AEV-NH937株组织毒1：100倍稀释，0.03ml/只脑内接种1日龄SPF雏鸡，分别在攻毒后1、3、5、10、14、20、25和30天迫杀实验攻毒组4只，对照观察组2只，分离血清，制备外周血、胸腺、法氏囊和脾脏的淋巴细胞悬液，同时采集脑组织，10%福尔马林固定，制备石蜡切片，做H.E。

The purpose of this study, on the base of former research, was to reveal and better understand some molecular pathogenesis of AE, to set up a working model which could be generally used by anther researcher in the field of animal molecular pathology. One-day-old SPF chicklings were inoculated via intracephalic injection at a dose of 0.03ml per chickling, consisting of 1:100 tissuer virus suspension.

本研究用AEV-NH937株组织毒1：100倍稀释，0.03ml/只脑内接种1日龄SPF雏鸡，分别在攻毒后1、3、5、10、14、20、25和30天迫杀实验攻毒组4只，对照观察组2只，分离血清，制备外周血、胸腺、法氏囊和脾脏的淋巴细胞悬液，同时采集脑组织，10%福尔马林固定，制备石蜡切片，做H.E。

And 4 of experimental group and 2 of control group were killed at 1, 3, 5, 10, 14, 20, 25 and 30 days postinfection, respectively. All the sera, the lymphocyte suspension of peripheral blood, thymus, Fabricius bursa and spleen, were collected.

本研究用AEV-NH937株组织毒1∶100倍稀释，0.03ml／只脑内接种1日龄SPF雏鸡，分别在攻毒后1、3、5、10、14、20、25和30天迫杀实验攻毒组4只，对照观察组2只，分离血清，制备外周血、胸腺、法氏囊和脾脏的淋巴细胞悬液，同时采集脑组织，10％福尔马林固定，制备石蜡切片，做H.E。

Quiescent RASMCs were incubated respectively in 10% FBS DMEM as control group, 10% FBS DMEM supplemented with 0.1% dimethyl sulfide as vehicle group and 10% FBS DMEM supplemented with panaxynol(at concentration of 1,3,9×10-6 mol·L-1) as panaxynol groups. 1. Cell counting and double time. Cell viability was examined with trypan blue exclusion test and the cell number was counted at 0, 24, 48, 72h after the incubation using a hemocytometer.

实验中细胞分成如下几个处理组：①正常对照组：含10％FBS的培养液；②溶剂对照组：0.1％DMSO；③药物处理组：panaxynol 1、3、9×10-6 mol·L-1组。1、细胞计数及倍增时间测定：于接种并同步化后处理0、24、48、72h，用血细胞计数板计算活细胞数量，绘制生长曲线并计算细胞倍增时间。

Seven days after inoculation, 1 mm tumor was formed on the back of 31 nude mice.

接种7 d 后，31只裸鼠背部形成直径约1 mm 左右的肿瘤。

Recombinant bovine prion protein was treated by chemical solution, and the treated Recombinant bovine prion protein was intracerebrally inoculated in the brain. The prion gene expression was quantified and the proteinase resistant protein was detected by western-blotting.

对重组牛朊蛋白进行了化学处理后脑内接种金黄地鼠，200天后对朊蛋白抗性进行了western-blotting检测，对脑组织朊蛋白基因的表达进行了分析。

Intradermal inoculation with C. neoformans is feasible for establishing a cutaneous cryptococcosis model in BALB/c mice.

BALB/c小鼠皮下接种隐球菌后发生原发性皮肤感染，可以作为研究本病的动物模型。

The growth status of the tumor was regularly observed, and the growth curve was recorded. 6 weeks later, all mice were executed by luxation.

收集各组细胞后接种于裸鼠腋下，建立有差异的裸鼠皮下移植瘤模型并绘制肿瘤生长曲线。

The survival time of the mice administrated retrobulbarly was 16±2d, the count of white blood cells and the percentage of immature granulocyte and monocyte obtained from tail vein did not raise, myeloblast and monoblast percentage of bone marrow was also in normal range.

由球后途经接种的小鼠其生存时间为16±2d，外周血白细胞计数、未成熟粒单核细胞百分率及骨髓原始粒单核细胞百分率一直处於正常范围内。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。