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After 20 days, at the end of fermentation, the cassava residue inoculated has better sensory quality, but the treatments of adding low levels of saccharose have low lactic acid accumulation amounts and high butanoic acid accumulation amounts compared with the treatments of adding high levels of saccharose, the treatment with inoculation and 4% saccharose addition has the highest lactic acid accumulation amount, which is 18.7 g/kg, and the treatment with inoculation and 8% saccharose addition has the lowest butanoic acid accumulation amount, which is 0.141g/kg.

结果显示:在发酵开始时接种处理的乳酸菌数为5×108 cfu/g,而对照处理没有检测到;发酵结束后,接种的3个糖水平处理的外观发酵品质比未接种处理好,但糖浓度低时丁酸积累较多,乳酸积累少,接种并添加4%蔗糖的处理乳酸积累量最高,发酵物中乳酸积累达18.7 g/kg,接种并添加8%蔗糖的处理丁酸积累最少,仅为0.141 g/kg。

At 48h to 96h PI, proliferative inflammation was observed. All the time, the nonsuppurative encephalitis was observed in the brain.

感染雏鸭的肝组织在接种后24h表现出血性坏死性肝炎变化,接种后48~96h呈增生性病变,而接种后各时期脑组织均呈非化脓性脑炎变化。

Researchers concluded,"This patient's positive rechallenge for seizure activity increases suspicion that varicella vaccine may be more than a coincidental factor in observations of postvaccinal convulsions."

研究人员断定,&这个病人对于惊厥行为刺激的反应度的激发增强了对这一事件的怀疑:在对接种后的抽搐观测中,水痘疫苗也许不止是一个巧合的因素。&

The cytochemical localization of POD showed that the peroxidase was found in cells ofboth cultivars after inoculation. The activity of POD in resistant cultivar was higher than that innot only the uninoculated controls but also the inoculated susceptible ones and localized morewidely, such as localized in mesophyll cell wall, intercellular spaces, membranes of chloroplastand mitochondrion and vacuolar membrane. Whereas the POD in susceptible cultivar waslocalized mainly in cell walls but little in plasma membrane with a lower range and intensitythan that in resistant cultivar.

对过氧化物酶的细胞定位研究发现,无论抗病杨树还是感病杨树,接种后均发现细胞中有过氧化物酶反应产物,通过分析发现,毛白杨过氧化物酶的活性不仅明显高于未接种的对照,也明显高于接种处理后的北京杨,并且定位更广泛,在细胞壁、细胞间隙、叶绿体膜、线粒体膜及液泡膜上均有大量定位,而北京杨酶反应产物主要分布于细胞壁上,在细胞膜、核膜局部有少量定位,定位范围及强度均小于毛白杨。

The minimum accumulation occurred at 96 h, at which transcripts were only 0.07 fold of that in uredinisopore. During incompatible interaction between Pst and wheat, PsCdc2 was down-regulated and its accumulation was lower than that in urediniospore.

在非亲和组合中,该基因表达基本呈下调趋势,在接种后各个时间点的表达量均低于在夏孢子中的表达量,其中接种后12 h时表达量最高,但仅为夏孢子中表达量的0.34倍;接种后96 h表达量最低,为夏孢子中表达量的0.02倍。

Real time RT-PCR analysis showed that in compatible interaction between Pst and wheat, PsCdc2 was up-regulated at early stage of infection. The maximum induction occurred at 12 hpi, at which transcripts were 1.62 fold over that in urediniospore. From 24 to 268 hpi, the accumulation of transcripts decreased steadily.

实时荧光定量RT-PCR结果表明,在亲和组合中,该基因在条锈菌接种小麦的前期上调表达,其中接种后12 h时表达量最高,约为夏孢子中表达量的1.62倍,接种后24-268 h,基因表达基本呈下调趋势,其中96 h基因表达量最低,仅为夏孢子时期的0.07倍。

The maximum activity appeared at 96h PI(23.70±7.65);in the dose-effective ness experiment,40 broilers were inoculated with different doses of sporulated oocysts respectively:there were 0,0.5×104,1.0×104,1.5×104,2.0×104, 2.5×104,3.0×104,3.5×104per chicken,iNOS activity was examined by the same method at the time with maximum iNOS activity detected in the experiment of time-effective relationship after PI.

试验结果表明,与对照组相比,肉鸡盲肠组织iNOS的活性在接种球虫后不同时间明显不同,其中接种后第96h活性最高(23.70±7.65);在剂量-效应关系试验中,40只肉鸡以不同的剂量0、0.5×104、1.0×104、1.5×104、2.0×104、2.5×104、3.0×104、3.5×104个/只分别口服接种,然后按时间-效应关系中酶活性最高的时间点取样,以同法检测iNOS活性。

With the giant 201 alfalfa varieties match highly effective nodulestrain is SM29, SM32, SM33 and SM35, after vaccinates above four kind of strains, entire nitrogencontentequally grew compared to the comparison 27.38%; A high comparison equally grew60.27%; The aerial parts dry weight grew 2~3 to double, separately grew 327.00%, 300.65%,280.56% and 279.70%; After the vaccination fresh weight and the comparison compares alsohas theremarkable enhancement, separately grew 148.12%, 132.64%,121.34% and 121.76%.

田间小区试验结果表明,与巨人201高效匹配的根瘤菌株是SM29、SM32、SM33和SM35,接种后其全氮含量分别为27.11%、26.78%、28.92%、27.5%,而对照仅为21.65%;地上部分干重与对照相比,有显著性的增长,分别增长了280.56%、327.00%、300.65%和279.70%,增长了1~3倍,有效地提高了紫花苜蓿的干草产量;接种后地上部分植株高度分别为102.67cm、100.17cm、94.25cm、102.50cm,而对照植株高度为62.33cm;接种这四种根瘤菌,接种效果明显,大大提高了巨人201的鲜草产量,分别比对照增长了121.34%、148.12%、132.64%和121.76%。

With conventional PCR primer pair XBFF/R,the same 352bp bands appeared by amplifying all the DNA samples from the eight races,and no bands appeared by amplifying all the DNA samples from the 9 plant pathogen species(Cucumber powdery mildew,wheat yellow rust,wheat leaf rust,wheat stem rust,wheat scab,wheat sheath blight,wheat loose smut,maize sheath blight and maize head smut)and control.

3用常规PCR和实时荧光PCR两种方法对温室接种的小麦白粉病进行了检测,并在时间上与自然显症的观察进行了比较,结果表明:在温室条件下,小麦白粉病菌在接种后第5d时开始出现小麦白粉病病斑,而利用常规PCR引物XBFF/R可在接种第3d检测到小麦白粉病菌,比自然显症观察能够提前了2d检测到白粉病菌;实时荧光PCR法可在接种后1d即能检测到小麦白粉病菌,比自然显症观察能够提前了4d检测到白粉病菌,这可为白粉菌的预测或预警检测提供依据,更可为白粉病在流行前制定防治决策赢得宝贵时间。4。

If requirements is met , after paying the fee , please wait in the Vaccination Room for twenty minutes , if there is no reaction to the vaccination , you can get the International Certificate of Vaccination after three working days.

符合接种条件的由医生开接种单,缴费后,到接种室接种。接种后观察20分钟,无不良反应方可离开。三个工作日后取证。

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