接种后的

Serum free medium containing 0,16,32,64,128/μg/mL was added 24 hours after culture and cultured for another 24 hours. Cells were collected centrifugally and resuspended in 75% alcohol. Finally, cells were fixed 4℃overnight. Then cells were stained with PI for 30 min under 4℃.. FCM was run with parameter FL2 - A.

流式细胞仪检测细胞凋亡率对数生长期的BIU-87细胞接种于培养瓶内，24 h后更换含有终浓度0、16、32、64、12μg/mL的无血清培养液，处理24 h，离心收集，75%冷乙醇悬浮后4℃固定过夜，PI染液4℃避光染色30min，流式细胞仪于FL2-A参数下检测。

The antibodies were injected intraperitoneally at a dose of 20 μg/mouse at day 1, 2, 3 after inoculation or at a dose of 100 μg/mouse once a week after tumors were detected.

抗体以20μg/只的剂量于接种肿瘤细胞后第1、2、3天腹腔内注射或者以100μg/只的剂量于检测到瘤体后每周1次腹腔内注射。

Good callus with high regeneration frequency can be obtained when scutellum of embryo at 25 days after pollination were used as expla...

最佳取材时间在开花后 15d左右，而对于在开花后 2 5d取的大龄胚，若仅挑取盾片组织进行接种，也可以获得质量较高和再生能力较强的愈伤组织

The virus titers in allantoic fluids decreased with freezing and melting and it reduced about 50% for each time.

接种病毒后死亡鸡胚的新鲜尿囊液经冻融后，其中鸽NDV的HA滴度和TCID50均逐渐下降。

Following attachment to the host immunized with purified antigens, midgut antigens, salivary gland antigens and reinfestation by I. sinensis, the midgut of tick revealed rather strikingly pathological changes, especially of the tick feeding on host immunized with 105KD purfied antigens the basal lamina became thinner, looser, and broken.

中华硬蜱叮咬纯化抗原、中肠抗原、唾液腺抗原免疫接种组和再次感染组宿主后，中肠可发生一系列明显的病理变化，尤以叮咬105KD纯化抗原组宿主后中肠病理变化最为严重。

Pale yellow, brown and white translucent calli were produced from zygotic embryos which were stored at 4 to 6℃ for 1 to 3 months and then cultured on medium LP+2mg/L 2, 4-D+1mg/L 6-BA in the dark for one month. Only white trenslucent calli were embryogenic.

合子胚在4～6℃低温条件下保存1～3个月后，接种于LP+2mg/L 2，4-D+1mg/L 6-BA的培养基上，黑暗条件下培养1个月后，产生浅黄色、褐色和白色半透明三种愈伤组织，其中白色半透明愈伤组织是胚性愈伤组织。

Hours after inoculation, penetration of the fungus and formation of haustoria in transformed cells were observed to evaluate the effects of the target gene's products on the invasion of powdery mildew.

转化后接种白粉菌孢子，48 h后观察转化阳性表皮细胞，研究抗病相关基因表达对白粉菌入侵及吸器形成产生的影响。

Methods The guts of embryonic mice removed and dissociated were plated into serum-free DMEM/F12 medium. The mitogen-free DMEM/F12 medium supplementd with 10% fetal bovine serum was used to induce differentiation of GNCSCs. Neurospheres and their derivations were determined with immunocytochemical and immunofluorescent staning.

分离胚鼠肠管，消化后接种于添加N2、B27和碱性成纤维细胞生长因子的DMEM/F12培养基，贴壁培养；连续传代后用血清促进GNCSCs分化；最后用免疫细胞化学方法染色鉴定。

Methods The guts of embryonic mice removed and dissociated were plated into serumfree DMEM/F12 medium. The mitogenfree DMEM/F12 medium supplementd with 10% fetal bovine serum was used to induce differentiation of GNCSCs. Neurospheres and their derivations were determined with immunocytochemical and immunofluorescent staning.

分离胚鼠肠管，消化后接种于添加N2、B27和碱性成纤维细胞生长因子的DMEM/F12培养基，贴壁培养；连续传代后用血清促进GNCSCs分化；最后用免疫细胞化学方法染色鉴定。

Methods The retinae of 6 postnatal 2-3 days Sprague-Dawley rats were dissociated into cell suspension with trypsin digestion. The cell suspension was implanted in 96-well culture plates covered with murine-tail collagen in each well(1×105 cells/well). The concentrations of PGP solution were 1g.L-1-10-5g.L-1. The period of culture was 1,3 and 5 days respectively, Then, the OD of living cells in each well was tested by MTT colorimetric microassay.

6只生后2-3d Sprague-Dawley大鼠，视网膜采用胰酶消化法制成细胞悬液后接种于经鼠尾胶原处理的96孔培养板(每孔1×105个细胞)，同时加入1g.L-1-10-5g.L-1PGP及DMEM，分别培养1、3、5d，用MTT法测量OD值。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。