接种后的

The apical buds and axillary buds of Dracaena cambodiana Pierre ex Gagnep. were inoculated on MS medium containing 1 mg/L BA, 0.1 mg/L NAA, 100 mg/L PVP and 30 g/L sucrose for inducing shoots. After cultured for 40~50 d, the shoots bourgeoned. They were then transferred onto MS medium supplemented with 2 mg/L BA, 0.5 mg/L KT and 30 g/L sucrose for inducing clustered buds. After 25~10 d, the clustered buds formed. The proliferation rate of the clustered buds was 300%~500% per month.

以海南龙血树的顶芽和侧芽作为外植体，把其接种于MS+BA 1 mg/L+NAA0.1 mg/L+PVP100 mg/L+蔗糖30 g/L培养基上培养40~50 d可诱导其腋芽萌发，再把萌发后所形成的新芽切割下来接种于MS+BA 2mg/L+KT 0.5 mg/L+蔗糖30 g/L的培养基上培养25~30 d可诱导形成丛生芽，丛生芽在继代培养过程中每25~30 d可增殖3~5倍。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

We also inoculated cottonwood clone 895 with 6 ectomycorrhizal fungi. It was found that Xerocomus chrysenteron, Lactarius insulsus and Boletus edulis accelerated seedlings generation from cuttings of clone 895 more than the other fungi did, and they remarkably promoted seedling growth comparing with the control. Lactarius insulsus promoted above-ground biomass and root system most effectively.

接种南林－895杨的6种菌根真菌中，红绒盖牛肝菌、劣味乳菇、美味牛肝菌可促进南林－895杨扦插苗提早萌芽，处理120 d后能显著促进南林－895杨苗的生长，与对照比较差异显著，其中劣味乳菇对杨苗生物量影响最大，其接种试验苗根系最为发达。

Oilseed rape were inoculated by spraying when they had three leaves, Tu-100 populations were not detectable on stems and leaves 20 days after spraying, and their resistance to Sclerotinia disease were decreased gradually with time.

在油菜三片真叶时喷雾接种一次，20d后，在茎和叶上不能检测到所接种的Tu-100，并且抗菌核病的能力也逐渐减弱。

Methods A random and open clinical trial was performed in children at more than 18 months of age, who were uninoculated with HA vaccine, and adults at 18~35 years of age in Yancheng City, Jiangsu Province, China.

采用随机、开放式试验设计，选择出生后未接种过甲肝疫苗的18月龄以上的儿童和18~35周岁的成人，接种冻干甲肝减毒活疫苗1针。

Objective To study the effect of Borna disease virus infection on the transcription of monoaminergic receptor genes in the brain tissues of neonatally inoculated rats.

目的分析博尔纳病病毒感染对新生大鼠脑内单胺类受体基因转录的影响。方法选择病毒滴度为2·0×106FFU/ml的BDV病毒液对新生大鼠进行颅内接种，接种量为10μl/只新生大鼠。30d后用RT-PCR和间接免疫荧光方法确定BDV感染情况；并采用半定量RT-PCR方法检测BDV感染大鼠脑组织中多巴胺2(D2)受体和5-羟色胺2α(5-HT2α)受体基因的mRNA转录情况。

Rabbit was immunized by the conjugated alginate-BSA (1.0mg/kg) for 40-days routine immunity method; ELISA method was used to examine the titration of anti-alginate serum; The first generation parotid acinar cells were chosen to divided five groups (group A :contrast, group B: BAS, group C: alginate, group D: anti-alginate serum, group E: alginate +anti-alginate serum), We examined the proliferation of each part by MTT method at four times (lh, 6h,12h and 24h); In the meanwhile, The growth and shape of parotid acinar cells were observed under phase contrast microscope and scanning electron microscope.16 of the immunized rabbits by alginate were divided equally into Al and Bl groups, 12 normal rabbits were divided equally into A2 and B2 groups as control groups, Each rabbit was affused by 0.3ml(40fil/ml)alginate though parotid duct once time two days.

将藻酸盐与BSA偶连形成具有较强免疫原性的Alginate-BSA交联物免疫兔，按抗原量1.0mg/kg接种于新西兰白兔皮内，间隔10d加强免疫1次，共加强4次后，利用间接ELISA法检测藻酸盐抗体效价：将原代培养的兔腮腺腺细胞按10~4个/ml接种到96孔板上，置入细胞培养箱继续培养3d，分为5组：A空白对照组、B牛血清白蛋白组、C藻酸盐组、D抗藻酸盐血清组和E抗藻酸盐血清+藻酸盐组，分别加入PBS、牛血清白蛋白、藻酸盐或新鲜抗藻酸盐血清继续培养，分别于1h、6h、12h和24h不同的时间点，取出96孔培养板，采用MTT法检测5组腮腺腺细胞增殖情况

Produce enriched apple juice yoghurt best machining process conditions for: 10 g sucrose, 93℃, adopted 25 min rinsers processing of milk, cooling after vaccination, bacteria with reassortant of 4%, in 37 conditions to develop 10 h, by adding 7% of condensed apple juice, in 4℃ conditions for refrigeration, product texture johol brisk, unique flavour, nutrient-rich, and health.

得出浓缩苹果汁酸奶的最佳加工工艺条件为：加入10g蔗糖，采用93℃、25min巴氏杀菌处理牛奶，冷却后进行接种，菌种接种量为4%，在37℃条件下培养10h，加入7%浓缩苹果汁，在4℃条件下进行冷藏，所得产品口感柔和爽快，风味独特，营养丰富，并具有一定的保健功能。

Hyphal growth rate method was used to determine the inhibitory activities of chitooligosancharide against the pathogenic fungus of tobacco brown spot, Alternaria alternate Keissler, in the laboratory, and tobacco plants were applied with COS and its combinations like COS + Trichoderma + chitin with 5 treatments to control the disease under conditions of pot culture and inoculation in the greenhouse. Activities of 4 defensive enzymes, superoxide dismutase, polyphenol oxidase, peroxidase and chitinase, in tobacco plants were determined systematically at the same time within 10 days after application of COS and inoculation with the pathogenic fungus.

在实验室条件下，采用菌丝生长速率法测定了几丁寡糖对烟草赤星病菌的抑制活性，继而在温室盆栽和人工接种条件下，分别研究了几丁寡糖、几丁寡糖+木霉+几丁质等5种方法处理对烟草赤星病的防治效果，同时分别系统测定了烟草植株施用几丁寡糖及人工接种赤星病菌后10 d内SOD、POD、PPO和几丁质酶等4种防御酶的活性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。