接种后的

Results The sympotoms of twinge , titillation , red halo ,urticaria and papular eruption were manifested on the local skin of upper limbs of volunteers after being touched by toxigenic spine of Eucleid Caterpillar . The dermatitis symptom on the skin of upper limbs of volunteers after being vaccinated directly by the abstergent liquid of toxigenic spine was similar to that of volunteers after being touched directly by toxigenic spine .

自愿者上肢皮肤被刺蛾幼虫毒刺毛触刺后，局部表现为刺痛，奇痒，红晕，风团，丘疹等；接种毒刺毛洗脱液后，自愿者上肢皮肤所出现的皮炎症状与毒刺毛触刺皮肤后出现的皮炎症状相似；而加热后再接种自愿者上肢皮肤，则不出现上述皮炎症状。

The results showed that typical FMD clinical signs produced in cattle inoculated intradermally with pathogenic FMDV after 48 h. The 3ABC antibody produced in these cattle were detected to be suspicious on the 3rd day, 3ABC antibody in the 1/4 cattle were positive on the 5th day, and the 4/4 were positive on the 8th day post virus inoculation; In 10 co-housed cattle (breeded with artificially infected pigs and sheep separately), no 3ABC antibody were detected on the 7th day and percent of positive 3ABC antibody was only 30%(3/10) on the 15th dpi (4/10 had clinical symptom).

实验表明，4头牛在舌面接种感染FMDV 48h后全数发病，第3天即出现可疑的3ABC抗体，第5天检测到阳性的3ABC抗体，至第8天3ABC抗体全部为阳性；10头同居牛（分别与接种感染猪和羊同居）在同居第7天时未检出3ABC抗体，在同居第15天时，3ABC抗体阳性率仅为30%；肌肉接种病毒羊3ABC抗体产生时间与接种病毒牛一致。

Methods: A 5ml bone marrow was extracted from the lilac of human volunteers. By Percoll fluid and density gradient centrifugation, the MSC was obtained; after the cells filled the bottom of vessel, subcultured them, when they subculture in third generation, redigested them, 500 R/min centrifugate, alter the completed medium to chemical definition medium, examined the form change and prolifration of cells by invert microscope, toluidine blue stain、immunocytochemical stain and RT-PCR to test the type Ⅱ collagen mRNA and proteoglycan.

取健康成人髂后上棘处骨髓5ml，经percoll液分离后密度梯度离心，〓／ml密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心5分钟，〓／ml接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质中的蛋白多糖，免疫细胞化学染色检测ECM中Ⅱ胶原的蛋白合成，RT-PCR鉴定诱导细胞Ⅱ胶原mRNA的表达。

METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜，剪碎后胰酶消化，制成单细胞悬液，按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中，18~20 h后将细胞悬液转移至另一未包被的25 cm 2玻璃培养瓶中（第1次差速贴壁），24 h后再将细胞悬液转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中（第2次差速贴壁），接种培养48 h后半量换液以去除杂细胞。

M treatment materials, and also tartaric acid and no maleic acid detected in G. v treatment materials. Inoculation also increased the content of organic acid secretion (increased by 19.80～56.87 mg/kg) of trifoliate root, and the application of AlPO4 greatly increased organic acid content (increased by 20.06～21.84 mg/kg); uninoculated plant root secreted a bit of organic acid; Mycorrhizal poncirus trifoliata seedlings excreted malic acid (42.87%), citric acid (39.22%) and oxalic acid (12.06%).

接种AM真菌的根系分泌的有机酸种类与对照有所不同，未接种处理枳分泌的有机酸有草酸、苹果酸、乳酸、乙酸、顺丁烯二酸和柠檬酸等6种，而接种G.m的则检测到草酸、酒石酸、苹果酸、乳酸、乙酸、柠檬酸、丁二酸等7种，G.v处理的检测到酒石酸，接种处理均未检测到顺丁烯二酸；接种丛枝菌根真菌增加了枳根系分泌有机酸的量比未接种处理增加19.80～56.87 mg/kg，且施用AlPO4后有机酸含量显著增加(增加20.06～21.84 mg/kg；未接种植株根系仅分泌少量有机酸；接种植株根系分泌的有机酸以苹果酸(42.87%)、柠檬酸(39.22%)和草酸(12.06%)为主。

The above facts indicated that silicon accumulation in the rice leaf sheath inside, the leaf surface and the sclerenchymatous cell, played the physical barrier role, delayed the hypha of sheath blight expansion.5、Analysing the physiological and biochemical mechanism of the resistance to rice sheath blight improved by silicon application in several respects.(1) After being inoculated with R.solani, O2 and POD activities disordered, CAT activities obviously declined. O2 producingvelocity in 91SP\'s leaves and leaf sheaths of Si+ rice plants were lower than those of Siriceplants, MDA content in leaves and leaf sheaths of Si+ rice plants were lower thanthose of Si- rice plants significantly in three days after inoculating, SOD、POD、CATactivities in leaf sheaths of Si+ rice plants were higher than those of Si- rice plantssignificantly in one day after inoculating, SOD、POD、CAT activities in leaves ofSi-treatment were higher than those of Si- rice plants significantly in three days afterinoculating. O2 producing velocity and MDA content in Lemont\'s leaves and leafsheaths of Si+ rice plants were lower than those of Si- rice plants significantly afterinoculating, and SOD、POD、CAT activities of Si+ rice plants were higher than those ofSi- rice plants significantly.

说明硅在水稻叶鞘内侧、叶表和厚壁细胞积累，起到了物理屏障的作用，延缓了纹枯病菌的扩展。5、阐明了施硅增强水稻抗纹枯病的生理生化机制(1)接种纹枯病菌后，两个水稻品种91SP和Lemont的叶鞘和叶片超氧阴离子自由基O2性紊乱，CAT活性明显下降；抗病品种91SP叶鞘和叶片施硅处理的O2产生速率小于缺硅处理，叶鞘和叶片MDA含量在接种第3 d后显著低于缺硅处理，叶鞘SOD、POD、CAT活性在接种1 d后一直显著高于缺硅处理，叶片SOD、POD、CAT活性在接种3 d后显著高于缺硅处理；感病品种Lemont叶鞘和叶片施硅处理的O2产生速率、MDA含量始终显著小于缺硅处理，SOD、POD、CAT活性始终显著大于缺硅处理。

Carmichaeli leaves were studied after spraying salicylic acid 2 days before inoculation with Septoria aconite Bacc.

用5mmol/L的水杨酸对附子抗病品种和感病品种分别进行诱导，两天后接种乌头壳针孢，研究处理后附子叶片相关酶活性的变化，结果表明，SA诱导后能明显增强附子叶片内POD、PPO、PAL的活性；诱导接种处理比对照接种处理酶活性增加更为明显，同时抗病品种比感病品种酶活性更强，增加幅度更大，高酶活持续时间更长。

Methods The purified general bacteria in broth culture for46hourswerepreserved in glycerol-physiological saline,the purified streptococcus and Neisseria scraped from the agar culture were preserved in fresh sheep-blood which was added suitable glycerol-physiological saline,the purified fungi were cultivated in half-solid culture;-20℃general bacteria and fungi,-80℃streptococcus and Neisseria.

将普通细菌的菌种纯化后接种于普通肉汤管，4～6h增菌后，加入适量甘油-生理盐水保存液，-20℃冰箱保存；链球菌属和奈瑟氏菌属的菌株纯化后，用接种环取菌置于新鲜脱纤维绵羊血中，加入适量保存液，-80℃低温冰箱保存；真菌菌种纯化后接种于带硅胶塞的半固体培养基，25℃培养24h后，-20℃冰箱保存。

It is seen from grafting Aphid test that green peach aphid can feed on green cabbage,spinach and lettuce,and reproduce on them,furthermore,it shows the different viability between on the tested hosts.

通过接种试验看出，桃蚜接种在青菜、菠菜、莴苣等蔬菜上均能取食繁殖，而且相互间可以接种，桃蚜接种在青菜上繁殖生长最快，萝卜蚜和桃蚜接种在番茄上则不能生活，青菜上的萝卜蚜和桃蚜接种在烟草上，其结果萝卜蚜不能生活，而桃蚜经过短期适应取食后，仍可生长、繁殖，为害烟草。

Ata28 strain was mutated by UV with the most optimum mutant time, including 10, 20, 30s, that was chosen by the trial tests, considering the lethality rate and positive mutation rate of Ata28; it was found that 15 mutant strains were shown antagonism against Tbs3 strain by antagonism test on PDA plate, inhibition zone of mutant strains exceeded the original strain Ata28, and the maximum of inhibition zone reached 9.4mm; the mutant strain Uv-126 was found most effective in tobacco brown spot control and a rate of efficiency control reached 52.2% on tobacco plants three days after bacteria spraying.

采用硫酸二已酯诱变Ata28菌株，通过预备实验，计算Ata28菌株在不同诱变时间的致死率和正变率，选择该菌株的最佳诱变时间20min，40min，60min诱变；通过平板对峙培养，筛选出17个突变菌株在PDA平板上可明显抑制Tbs3的生长，抑菌带高于出发菌株Am28，抑菌带最大值达10.5 mm；经温室控病初筛和复筛试验，获得了对烟草赤星病有较好防效的突变菌株Des-66、Des-50，烟苗经Des-66、Des-50菌液处理3d后，接种Tbs3的防治效果达52.0％、56.6％，比出发菌株Ata28的防治效果好。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。