探针

Cytoplasmic actin microfilament systems were localized in the freshly isolated onion protoplasts of bulb parenchyma cells using TRITC labelled phalloidin probe in the present study.

使用四甲基罗丹明标记的鬼笔环碱探针，以新分离的洋葱鳞茎薄壁细胞原生质体为材料，观察了细胞胞质肌动蛋白微丝骨架的结构与形态。

By using the results from systemical petrography, X-ray powder diffraction, and EPMA analyses on the run products from experimental simulaion under constant temperature and pressure, we have explored the paragenesis for the pelites suffering metamorphic dehydration under high pressure condition, the process and mechanism for these metamorphic dehydration, and the relation between the components for garnets, one of the major phases in the paragenesis, and the experimental conditions including pressure, temperature, and chemical compositon in a system.

由恒温、恒压实验产物系统的岩相学、X射线粉晶衍射和电子探针分析，揭示了天然泥质岩发生高压变质脱水作用后的矿物相组合，确定了泥质岩高压变质脱水作用的过程和机理，以及主要矿物相石榴子石的组成与压力、温度和体系化学组成的关系。

Structural analysis is of great importance to further study the transport and magnetic properties of these manganese oxides.

通过四探针技术测量了系列样品在不同磁场下的电阻率，实验表明在低磁场下的电阻率变化不明显。

The pressure transducers and ion-probes were utilized to measure the pressure and velocity in the tube, respectively.

利用安装在同一截面上的压力传感器与离子探针同时测得DDT位置段爆震管内的压力分布、火焰传播速度和方向。

The ion probes and pressure transducers were utilized to measure the flame velocity and pressure in the tube, respectively.

利用压力传感器与离子探针同时测得爆震管内的压力和火焰传播速度。

They are subjected to the base pairing rules of Watson-Crick and Hoogsteen, while they are resistant to digestion and degradation by nucleases and proteases.

根据PNA的生物特性，以PNA为基础的PNA探针和PCR钳制技术在肿瘤和老化研究以及染色体畸形、细菌和DNA点突变诊断等方面的应用日益增多。

Because artificial nucleases can cleave nucleic acids at specific sequnce or site, it can be used as very important tools in molecular biology, can be applied to gene therapy, purity of proteins, anti-sense PCR technique, DNA footprinting and chemical probe, and so on.

由于人工核酸酶可在核酸底物的特定部位将其断裂，所以它可以作为重要的分子生物学工具，可应用于疾病的基因治疗，蛋白质分离纯化中去除核酸，还可用于反义PCR技术、DNA足迹技术、化学探针等。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

The distribution of nitrogen concentration and the microstructure of the nitrided layer were measured and observed by EPMA and SEM respectively.

在氮气中对304奥氏体不锈钢进行固溶渗氮，用电子探针测定了渗氮层的氮浓度分布，用扫描电子显微镜观察了渗氮层的金相组织。

The response time can be shortened and high throughput and real-time detection analysis can be achieved. Although now the use of zero-dimensional nanomaterials is popular, it is still rare for the study of one-dimensional materials.

与传统的生物传感器相比，基于纳米技术的新型生物传感器已呈现出更优越的性能，具有了亚微米的尺寸、换能器、探针或者微米系统，生物传感器的化学和物理性质和其对生物分子或者细胞的检测灵敏度大幅提高，响应时间也得以缩短，并且可以实现高通量的实时检测分析。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。