探针

The study of spatial configuration and characteristics of pulsed-wire velocity probe shows that: the yaw-and pitch-response characteristics have a good behavior due to the asymmetrical arrangement of the prongs. Because the amplitude of sensor wire response signal changes with the flow velocity, the differentiated signal is selected to trigger a voltage comparator. The pulsing frequency is restricted to about 100Hz to allow the wire to cool between pulses. The first velocity probe with big acceptance cone has been developed in China, and the way of measuring the Reynolds normal and shear stress and application have been given in this dissertation.

对脉冲热线空间速度探针的空间结构及特性的分析表明：三根长支架平行且安置于同一平面的探针结构可以获得大的测量角和好的测量性能；由于传感丝的响应信号幅值随流速变化较大，故应采用微分法进行信号处理；对于5μm的传感丝由于散热时间基本不变，约为10ms，故脉冲发射的最高频率为100Hz；本文在国内首次研制成功了一种大测量窗的脉冲热线空间速度探针，并给出了测量雷诺应力的方法和实例。

In this thesis, we did modifications of alkyl groups upon benzyl fluorophosphonate. In order to study the influence of alkyl length and steric effect on activity and selectivity of probes, we synthesized benzyl fluorophosphonate recognition heads with modifications including ethyl, butyl, octyl and cyclohexyl. By way of connecting different kinds of tag like azido group, biotin and rhodamine with recognition heads separately through a higher hydrophilic ethylene glycol derived linker, a series of benzyl fluorophosphonate probes were made up. And they will apply to various biological testing strategies.

在本论文中，我们以苯甲基氟化磷酸酯为基础，於其上进行一系列烷基之修饰，为了探讨烷基长度与空间立体性质对於探针活性及选择性的影响，我们合成了包含乙烷基、丁烷基、辛烷基以及环己烷基修饰的苯甲基氟化磷酸酯辨识端，并藉由具较高亲水性的聚乙二醇衍生连接桥结合三种不同的发报端，分别有叠氮基团、生物素及萤光基团来构成不同的化学探针组合，这一系列苯甲基氟化磷酸酯类化学探针将可被应用於不同的生物测试策略。

The fluorescence images that RhoCr1 coordinated with Cr~(3+)in PC12 cells showed RhoCr1 to be capable of penetrating into living cells and imaging intracellular Cr~(3+)changesFive probes have been designed and synthesized,where different aldehydes were covalently linked to rhodamine B dyes with diethylamine flexible chains.

吸收和荧光光谱的测试结果表明，探针RhoCr1在Tris-HCI中性缓冲溶液中能高选择性的识别Cr~(3+)并产生了荧光增强，通过对探针光谱性能的测试和TEPN滴定实验表明，探针RhoCr1与Cr~(3+)的络合是一个可逆的过程。

In the water samples from Gudao, and No. 1 and 2 wastewater stations of the Shengli Oil Field, the ratios of amount of iron bacterial cells to total microbial cells, detected by the combination of the 4 probes and DAPI staining, were 11.0%, 12.8% and 9.0%, respectively. In the iron bacterial community, Leptothrix, Sphaerotilus and L. ferriphilum were dominant in the 3 stations, and Leptothrix and Sphaerotilus, and L. ferriphilum cells to accounted for 2.94%±0.52%~3.39%±0.52% and 2.24%±0.16%~2.63%±0.49% of the total microbial cells, respectively, while L.

结果表明，利用基因探针能快速对胜利油田外排水中的铁细菌进行检测：在孤岛采油厂、胜利采油厂1号和2号综合处理站的外排水中，利用4种探针的组合探针检测到的铁细菌细胞数量分别占样品中微生物总细胞数量的11.0%、 12.8%和9.0%，其中Leptothrix和Sphaerotilus，以及Leptospirillum ferriphilum为胜利油田外排水中的3种优势铁细菌，分别占水样中总微生物细胞数的2.94%±0.52%~3.39%±0.52%和2.24%±0.16%~2.63%±0.49%；而Leptospirillum ferrooxidans和Acidithiobacillus spp。

Objective To establish a sensitive and specific gene probe method for detection of Staphylococcal enterotoxin B gene.

目的 建立一种灵敏度高、特异性好的DNA 探针检测葡萄球菌肠毒素B 基因方法。方法用聚合酶链反应扩增的方法进行葡萄球菌B 型肠毒素基因探针生物素标记，用制备好的基因探针，进行斑点杂交，对葡萄球菌肠毒素10 株标准株和39 株临床株分别进行了检测。

Second Municipal People's Hospital of Guangzhou, Guangzhou 510150 Objective To study the technique and diagnostic value of fluorescence insitu hybridization in chromosome abnormality for prenatal diagnosis. Methods Amniocenteses were performed in 34 pregnant women of 16～23 gestational weeks with indications for prenatal diagnosis. The amniotic fluid samples were cultured in Chang's medium.

对34例孕16～23周有产前诊断指征者经腹部抽取羊水，用Chang培养液传代培养，常规制备羊水细胞分裂中期染色体，应用生物素及地高辛标记的人类全着丝粒探针和X、Y、13、21、18号染色体α-着丝粒探针及染色体全涂染探针，与培养的羊水细胞分裂中期染色体DNA进行原位杂交，杂交后用荧光显微镜观察玻片并摄像。

Methods Amniocenteses were performed in 34 pregnant women of 16～23 gestational weeks with indications for prenatal diagnosis. The amniotic fluid samples were cultured in Chang's medium. The metaphase chromosomes were hybridized insitu with the human centromere probes,α-satellites DNA probes of X, Y,13, 21, 18 chromosomes and all primer chromosome probes. These probes were conjugated by Biotin and Dioxin then. The treated slides were examined and taken photos under the fluoromicroscope.

对34例孕16～23周有产前诊断指征者经腹部抽取羊水，用Chang培养液传代培养，常规制备羊水细胞分裂中期染色体，应用生物素及地高辛标记的人类全着丝粒探针和X、Y、13、21、18号染色体α-着丝粒探针及染色体全涂染探针，与培养的羊水细胞分裂中期染色体DNA进行原位杂交，杂交后用荧光显微镜观察玻片并摄像。

Mask Laser Writer, Probe card ,Reticle Case, Test Equipment for Memory, CMP, E-Beam Inspection,Ion Implantation , Optical Defect Inspection , Optical Probe, Therma Probe,Test Reticle Mask

激光绘图光刻机，光学探针，探针卡，掩膜盒，DRAM测试系统，电子束检测设备，光学缺陷检测设备，热探针，测试掩膜版

Label with LC-Red640 and LC-Red750 two are aimed at above all 112 code with 158 child detect 5 ′ carry bougie, corresponding anchor decides bougie to be labelled by fluorescence in 3 ′ end, circulate quickly from inside DNA of person gene group again PCR is distinctive the enlarge that make thing adds part of ApoE gene 265bP and with distinctive bougie cross hind, cause fluorescent resonance energy to transfer, the occurrence that passes different source cross and fault match alkaline base the fusible parameter of the type will finish feral model divide with choppy gene model.

首先以LC-Red640和LC-Red750标记两条针对112和158位编码子的检测探针5′端，相应的锚定探针在3′端被荧光标记，再从人基因组DNA中快速循环PCR特异引物扩增apoE基因265bP片段并和特异探针杂交后，引发荧光共振能量转移，通过异源杂交的出现和错配碱基的类型的熔解参数来完成野生型和突变基因分型。

The results show that compared with the nonmetal probe, the metal probe has a larger field enhancement effect, and its scattering wave induced by field enhancement has a bigger decay coefficient.

结果显示，相对于非金属探针，金属探针具有更大的场增强效应，并且探针产生的散射波的衰减系数也更大。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。