探针

The study on the phylogenetic relations among these species is very useful for the study on genome and marker-assisted breeding in Brassica species. The karyotypic analysis is one of the most important methods in analysing the phylogenetic relation of species with closer relations. The protocol for inducing high frequency metaphase was established by pretreatment with low temperature and 8hydroxyquinoline, and then the preparation of chromosome and karyotypes analysis of six economical important Brassica species were carried out. According to the karyotypic analysis, it was shown that in the genomes of amphidiploid species, there are genomical components from didiploid species. Our result showed that B. campetris, B. juncea and B. napus belonged to class 2B, while B.

以二倍体B.nigra（B，n=8）基因组为探针，用B.campestris（A，n=10）或B.oleracea（C，n=9）的基因组进行封阻，分别与B.juncea（AB，n=18）和B.carinata（BC，n=17）进行GISH杂交发现，B.juncea和B.carinata基因组中仅有来自于B.nigra的8对染色体表现杂交信号，而其它染色体杂交信号较弱；以B.campestris或B.oleracea基因组为杂交探针与B.napus（AC，n=19）进行GISH杂交发现，所有19对染色体均出现杂交信号，根据杂交结果难以正确判断B.napus基因组中的哪些染色体是来自于A基因组，哪些是来自于C基因组；以rRNA基因（18S-5.8S-26S）为探针的FISH杂交结果表明，B.campestris基因组中rRNA基因位点为6对；B.oleracea为3对；B.nigra为2对；B.carinata为4对；B.juncea为6对；B.napus为6对。

In this paper, the Affymetrix gene expression array has been taken as an example and the problems of mismatch and non-specificity existing in the probe annotation of DNA microarray were analyzed.

本文以Affymetrix基因芯片为例，分析了基因芯片探针注释中存在的探针错配以及探针非特异性两个主要问题。

To suit above asks on international, get stuck to bougie (the design that the making science and technology of Probe Card) used perpendicular array, science and technology of advanced laser small treatment was used in the making craft that bougie blocks, the bougie of unifinication of research and development gets stuck.

国际上为适应以上要求，对探针卡的建造科技采用了垂直阵列的设计，在探针卡的建造工艺中采用了前进前辈的激光微加工科技，研发一体化的探针卡。

Former the bougie that the bougie of a cross can be isotropy, with radiate to detect from develop the bougie that; also can be blame isotope, give through fluorescence or enzymatic law detect.

原位杂交的探针可以是同位素的探针，用放射自显影来检测；也可以是非同位素的探针，通过荧光或酶法予以检测。

Get stuck with the bougie that checks at chip will from with bit the needle gets stuck and clip of annulus oxygen shot is given priority to to perpendicular type bougie card reachs filmy bougie to block development.

用于芯片测试的探针卡将从以刀片针卡和环氧针卡为主向垂直式探针卡及薄膜探针卡成长。

Some improvements are performed on the capacitive coupling probe to increase its frequency range. In this case, it could measure the D. C. volts and RF volts respectively.

针对原有容性探针存在的问题，增宽了新研制的容性探针的频响范围，改进后的容性探针能同时测量直流电位和射频电位。

This paper presents several kinds of IC Wafer testing probe card for Semiconductor Packaging and chip test.There are epoxy ring probe、vertical probe card micro spring probe card,and MEMS probe card.

本文简单介绍半导体产业集成电路芯片测试用探针卡，分为以悬臂梁方式的环氧探针、垂直探针和微弹簧丝探针，以及基于微机电系统的MEMS probe card。

In order to develop new DNA probes,we have synthesized two kinds of probes bipyrene ratiometric fluorescence probes and long wavelength Ruthenium polydiimine complex,the following work have been carried out:Fluorescent DNA probes with 1,6-hexanediyl as the linker between two pyrenes, phenylpyrenes or phenylethynyl pyrene fluorophores were synthesized(Py-1,Py-2 and Py-3) and their interactions with DNA were studied by UV-Vis absorption spectra,fluorescence spectra and viscosity measurements.

在前人的基础上，为了更好的识别DNA，本论文设计合成了两类DNA探针——双芘类比率型DNA荧光探针和长波长钌的配合物，具体如下：以己二胺为柔性链，以芘及其衍生物为发色团合成了探针Pyl-3，并通过荧光光谱，荧光紫外吸收光谱和黏度测试研究了探针与DNA的结合方式。

And a fluorescence molecule beacon probe is designed between upstream and downstream priers, 5' end of probe can mark fluorescence reporter group FAM, and the 3' end markes fluorescence quenching group DABCYL, and 5' end and 3' end of the probe have 6 bases which can be reflected and complemented to make fluorescence molecule beacon probe do not give out fluorescence signal in free state.

在上下游引物中间设计一条荧光分子信标探针，探针的5'端标记荧光报告基团FAM，3'端标记荧光淬灭基团DABCYL，探针的5'端和3'端有6个碱基能反折互补，使荧光分子信标探针在游离状态下不发出荧光信号。

It is indicated that only a single resonance peak appears in the amplitude versus frequency curve without spurious resonance peaks during the procedure. The direct actuation enables to improve the tuning accuracy, weaken the possible sample damage by the tip, and enhance the atomic force microscope bandwidth and imaging rate.

研究表明，采用压电探针的直接激振方法在探针振动的幅频曲线中仅有单一谐振峰，可以避免在压电陶瓷管激振方法下由液体振动造成的寄生谐振峰，因此使用压电探针不仅提高了探针振动频率的控制精度，而且减小了针尖对样品的损坏，还提高了仪器的带宽和成像速度。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。