探针

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16～24周的孕妇血中分离出NRBCs，并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果，3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%；其PCR结果，分别为阳性、阴性、阳性，并在引产后得到性别证实。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16～24周的孕妇血中分离出NRBCs，并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果，3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%；其PCR结果，分别为阳性、阴性、阳性，并在引产后得到性别证明。

Additionally,this study developed three species-spcific real-time fluorescence quantitative PCR-based assays for rapidly, specificity, sensitivity,detecting Brucella abortus, Brucella melitensis, Brucella suis.

在建立布鲁氏菌属荧光定量PCR检测方法的基础上，根据布鲁氏菌基因组多拷贝插入元件IS711及其下游多态位点，设计了检测牛、羊、猪种布鲁氏菌的引物和牛、羊、猪种特异性荧光探针，优化了牛、羊、猪种布鲁氏菌荧光定量PCR反应体系和条件，建立了牛、羊、猪种布鲁氏菌荧光定量PCR检测方法，组装了牛、羊、猪种布鲁氏菌检测试剂盒，该方法能特异检测上述3个种的布鲁氏菌。

The results observed showed that pathological changes consisting of destructive meningoencephalitis and large, basophilic, intranuclear and acidophilous intracy toplasmic neuronal inclusions of viral type occurred in the fetal mouse brain.

待雌鼠临产时剖腹取出胎鼠脑双侧大脑皮层，进行病毒分离、病理学检测及用地高辛标记的HCMV寡核苷酸探针对大脑皮层细胞压印片进行原位分子杂交检测。

Through electron probe\'s analysis to the albite jade, union grinding sample thin slice under polarizing microscope observation as well as X powder crystal diffraction data analysis. Discovery like Chen zhenqiang and "System Gemology" said that secondary mineral for actinolite, green mudstone, epidote and quart.

通过对钠长石玉进行电子探针的分析，结合磨制的样品薄片在偏光显微镜下的观察以及X粉晶衍射数据分析，可知钠长石玉中的主要成分为钠长石，次要矿物为硬玉、角闪石、绿辉石、钠铬辉石；并未发现如陈振强等人以及《系统宝石学》中所说的次要矿物为阳起石、绿泥石、绿帘石、石英。

However, for the cantilever with shorter effective length, there are several additional reasons for the error, e.

对短有效长度微悬臂梁，其误差原因主要为支撑的影响以及测试探针的大变形。

Shear force scanning near-field optical microscopy on biological materials is more critical to implement in liquid than in air because of viscous damping and sample softness.

基于剪切力的探针-试样间距控制扫描近场光学显微镜用于生物材料在液体中的成像比在空气中难得多。

The electrode tip is in a cone shape with radius of 0.5mm which attached to the electrode base with spring, providing cushion for better contact with human skin.

探针内附弹簧与探针套筒组成具弹性的电极，能使电极与皮肤有较理想的接触。

As a result,1A360 probe did hybridize strongly to Tester DNA sample,but weakly to Driver DNA.Thus,our two rounds of RDA are proved to be specific andeffective.Because of not hybridizing to the liver tissue DNA of rat and the thymicDNA of bull,so the probe is species-specific and gained from human chromosome.

该探针不能与鼠肝组织和小牛胸腺组织DNA杂交，说明它来源于人源染色体并有种属的特异性。1A360基因片段探针还与多种胃癌细胞系，胃癌组织DNA有较强的杂交信号，与其它肿瘤DNA有强弱不等的杂交信号。

In this invention, materials are five rare stones die qiao, drum stick, tassel, iron sheet and gold hair pin. The method is Chinese medicinal plant gDNA sequence specificity tiny array chip preparation method (its patent application number is 03132205.0). Specificity probe is got after sieving.

本发明是以五种名贵石斛即迭鞘、鼓槌、流苏、铁皮、金钗为材料，以我们创立的&中药材gDNA序列特异性微阵列芯片制备方法&(专利申请号：03132205.0)筛选获得的特异性探针，用这些特异性探针制备成微阵列进行名贵中药材石斛不同品种的鉴别。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。