探针

Then in situ hybridization of metaphase karyotype of male tufted deer are made use this two kind probes.

利用这两种探针分别对雄性毛冠鹿的中期染色体进行原位杂交，涂染探针的杂交得到了阳性结果。

The uniform electric field acting on all part of the tip above the infinite plane results in using the cone mode to interpret the experimental results.

在无穷大平板上，表面静电荷所生成的电场是均匀分布的，作用在探针上是影响探针整体，所以可用圆锥体模型来解释实验结果。

This suggested there might have cis-acting element in 3'-UTR. In order to search the possible trans-acting factor, we found RNA-protein band in H〓 cells and no band in 95D cells using 3'-UTR probe by RNA EMSA assay. At the same time, this band could be competed with the unlabled probe. There was also no band obsearved using 5'-UTR probe in both cells.

为了寻找可能与3'-UTR结合的蛋白质反式作用因子，用RNA凝胶阻滞(electrophoresis mobility shift assay，EMSA)的方法，我们在H〓细胞的胞浆蛋白质提取物与3'-UTR探针的结合反应中，发现了一条RNA-蛋白质的结合带，而在95D细胞没有结合带出现；而且这种结合带能被未标记探针竞争性抑制；5'-UTR的结合反应也没有观测到结合带的形成。

Mineral deposit clusters;(4) establishment of the theoretical framework of continental accretionary theory and prediction for mineral deposit clusters and recognition of mineral deposits.

按照 &大陆动力学过程－壳幔相互作用－增生成矿理论－预测方法体系&的研究思路，以&岩石探针&和&矿床探针&为切入点，研究大陆动力学演化过程和壳幔作用成矿机理，实现成矿理论和预测方法体系的创新。

It was used for detection of virus RNA in AIV infected chicken embryo allantoic fluids clinical specimens from various avian.

建立并优化了检测禽流感病毒核酸的探针杂交法，探针杂交法能鉴别出非免疫鸡胚和SPF鸡胚尿囊液中的病毒，攻毒后第 3天的 SPF和非免疫鸡泄殖腔拭子中 AIV的最大检出率为 1/10 ，对临床样品中的 AIV的最大检出率为 1/7，而直接 HA和 HI法及 AGP试验检不出临床样品的 AIV。

When there was no thrombin, aptamer probes could hybridize with molecular beacons in the highest degree, generating the largest fluorescence signal; when thrombin was introduced, the hybridization of molecular beacons and probes was depressed, and the depressing degree was associable with the concentration of thrombin.

没有凝血酶存在时，识体探针可以和分子信标最大程度地杂交，使得分子信标的荧光信号上升；当存在凝血酶时，与凝血酶孵育结合后的识体探针再与分子信标作用，分子信标被打开的程度有所降低，并且打开程度的降低程度与凝血酶浓度有关。

These excellent properties of 9E-BHVC all mentioned above imply that it would be a true two-photon nucleic acid fluorescent probe if it can specially recognize the DNA in complex cellulous environment.So this dissertation has investigated the ability of 9E-BHVC on the cell nucleus imaging detaily.

与BMVC相比，9E-BHVC表现出作为双光子荧光探针更好的特性，例如具有更大的活性吸收截面和更高的结合常数，且具有更好的水溶性。9E-BHVC这些优良的性质预示着该分子如果能够在复杂的细胞环境中特异性地识别DNA，那么它将是真正意义的双光子核成像探针。

Compared to BMVC,9E-BHVC has many better features needed by a two-photon probe,such as larger two-photon action cross sections,stronger affinity to DNA and better water-solubility.These excellent properties of 9E-BHVC all mentioned above imply that it would be a true two-photon nucleic acid fluorescent probe if it can specially recognize the DNA in complex cellulous environment.So this dissertation has investigated the ability of 9E-BHVC on the cell nucleus imaging detaily.

与BMVC相比，9E-BHVC表现出作为双光子荧光探针更好的特性，例如具有更大的活性吸收截面和更高的结合常数，且具有更好的水溶性。9E-BHVC这些优良的性质预示着该分子如果能够在复杂的细胞环境中特异性地识别DNA，那么它将是真正意义的双光子核成像探针。

GISH analysis showed Triticale have 2 items chromosomes presented yellow and others chromosomes presented chrysoidine, because yellow chromosomes combined the Thinopyrum intermedium DNA, others chromosomes dye by PI. 4. Analysis of resistance gene: CH-9916 is the hybrid progenies of Octoploid tritielytrigia TAI-7045 X common wheat "Jinmai33".

GISH分析结果表明，小偃麦衍生系CH-9916的42条染色体中有2条外源染色体因结合有探针DNA而呈现出较强的黄色杂交信号，而没有结合上探针DNA的普通小麦染色体经过PI染色呈现出桔红色，初步推断小偃麦衍生系CH-9916为一个&小麦一中间偃麦草&的代换系。

To improve detection efficiency and result accuracy, we developed an improved multiplex PCR technique for specific detection of GMO.①The assay strategy involves multiplex PCR amplification of the target with several biotinylated primer pairs in one tube, followed by the hybridization of multiplex PCR products and membrane-bound oligonucleotide probes. The multiplex PCR/probe hybrid is detected colorimetrically using an alkaline phosphatase conjugate and a chromogenic substrate.

在多基因定性检测技术方面，本文在优化并确定了多基因PCR反应条件的基础上，采用了两种方法对多基因PCR产物进行鉴定：①采用共价结合方法将多个探针固定在Biodyne〓C膜上，将5'端标记生物素的多基因PCR产物与膜上的探针进行杂交及显色反应，首次建立了GMO的多基因PCR-膜相杂交检测方法，实现了一个反应体系中同时检测多种转基因成分的目标，提高了检测效率与准确率。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。