掉下

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2－4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L－1 after their number counted.

选取2～5代的小鼠胚胎成纤维细胞，待其至对数生长期倒掉或吸掉培养基，加入含10 mg/L丝裂霉素C的细胞全培养液5 mL，置37 ℃，体积分数0.05的CO2饱和湿度温箱中培养二三小时，在6孔板中加入0.1%明胶包被，放置30 min以上，倒掉或吸掉含丝裂霉素C的培养基，磷酸盐缓冲液清洗5遍，尽量除去丝裂霉素C，加入2 mL 0.05%的胰蛋白酶消化细胞，镜下观察，当细胞间出现裂隙，细胞变圆时（约2～4 min），立即加入等量的全培养液终止消化，并用吸管反复吹打，使之成为单细胞悬液，在细胞计数板中央放置专用的盖玻片，用玻璃虹吸管吸取细胞，让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液，至盖玻片下被液体充满为止。

I am a wilful child perhaps My mother was spoiled child I wayward I hope Every moment As beautiful as crayons I hope The white paper in love can draw Draw clumsy freedom Draw a never Tears in eyes Sky A sky plumage and leaves A green night and apples I want to paint in the morning Under the picture Can see the smile Under the picture all the young No pain of love She hadn't seen clouds Her eyes are clear color She looked at me forever Forever, watching Never suddenly fell away I want to paint distant scenery Under the picture of the horizon and the water is clear Painted many happy river Under the picture Lightly covered with fluff I let them get very close Let them fall in love Let each one Each a silent spring throb Have become a little birthday I also want to paint in the future I haven't seen her, it is impossible But know that she is very beautiful I painted her coat of autumn Under the burning candle painting and maple leaf Painted many because love her And out of the heart Under the picture Draw a woke up early The glass wrappers above And the fairy tales illustrations I am a wilful child I want to wipe away all the unfortunate I think in the earth Painted with Windows Let all habits dark eyes All light I want to paint surface Draw a frame under a higher than the mountains Draw down the Orient Under the picture A voice of limitless Finally, in the paper Angle I also want to paint Draw a koala He sat on the dark jungle in Victoria Sit quietly branches Become speechless He had no home No one in the distance He only, many The same dream. Berries And a big, big eyes I hope think But I wonder why I didn't receive crayons Don't get a color moment I only have me My fingers and trauma Only the pieces Love her Let them go looking for a butterfly Let them vanish from today I was a child A mother is spoiled child fantasies I wayward

我是一个任性的孩子也许我是被妈妈宠坏的孩子我任性我希望每一个时刻都像彩色蜡笔那样美丽我希望能在心爱的白纸上画画画出笨拙的自由画下一只永远不会流泪的眼睛一片天空一片属于天空的羽毛和树叶一个淡绿的夜晚和苹果我想画下早晨画下露水所能看见的微笑画下所有最年轻的没有痛苦的爱情她没有见过阴云她的眼睛是晴空的颜色她永远看着我永远，看着绝不会忽然掉过头去我想画下遥远的风景画下清晰的地平线和水波画下许许多多快乐的小河画下丘陵长满淡淡的绒毛我让它们挨得很近让它们相爱让每一个默许每一阵静静的春天悸动都成为一朵小花的生日我还想画下未来我没见过她，也不可能但知道她很美我画下她秋天的风衣画下那些燃烧的烛火和枫叶画下许多因为爱她而熄灭的心画下婚礼画下一个个早早醒来的节日上面贴着玻璃糖纸和北方童话的插图我是一个任性的孩子我想擦去一切不幸我想在大地上画满窗子让所有习惯黑暗的眼睛都习惯光明我想画下风画下一架比一架更高大的山岭画下来东方民族的渴望画下大海无边无际愉快的声音最后，在纸角上我还想画下自己画下一只树熊他坐在维多利亚深色的丛林里坐在安安静静的树枝上发愣他没有家没有一颗留在远处的心他只有，许许多多浆果一样的梦和很大很大的眼睛我在希望在想但不知为什么我没有领到蜡笔没有得到一个彩色的时刻我只有我我的手指和创痛只有撕碎那一张张心爱的白纸让它们去寻找蝴蝶让它们从今天消失我是一个孩子一个被幻想妈妈宠坏的孩子我任性

He had travelled some miles, his horse and he, and he was feeling drowsy in the hot sunshine, when the horse stopped, lowered his head, and began to nibble the grass; and Toad, waking up, just saved himself from falling off by an effort.

他和马已经走了好几哩路。炙热的太阳晒得他昏昏欲睡。那马忽然停下来，低头啃吃青草。蟾蜍惊醒过来，险些儿掉下马背。

Ment of a leaf or pebble, or the bending down ofa spear of grass, or the removal of a little dust fromthe fence, is enough to give him the clew. Hesand, and carries the impression in his eye till a

他的洞察力是那么敏锐，以至于一片叶子或一块卵石的移动，或是一片被压弯的青草，或是篱笆上掉下的一点灰尘，都足以给这个眼睛提供线索。

A chip off the old block, I promise you!

从旧木块上掉下的碎屑，我保证！

Another is chip off the old block.

其他是从旧木块上掉下的碎屑。

People often say dewdrops are tears of stars.

都说那露珠，是星星掉下的泪滴。

Apartment dwellers in Tulsa, Oklahoma got this visitor from above: a dummy rocket off an Air National Guard jet.

俄克拉荷马州塔尔萨的居民迎来了这样一位访客：从国民空军警卫队的喷气机里掉下的火箭模型。

He takes a bit of a skydive, but I wouldn't say it is a cliff-hanger," Fichtner says."

他冒了一点险，但是我不觉得他就那样了，& Fichtner 说，&他要么掉下悬崖，要么就爬上去。

But the cat who does not listen, did not expect that at the foot of a hole, and then the cat-hole leader to go on falling, black rat smile ran away.

但是猫们不听，没想到脚下是一个洞，然后猫队长就掉下洞去，小黑鼠笑了笑就跑了。

The reasons of iron ions content overproof in grade Ⅱ desalting water system,such as variation water quality,contamination of regenerant , operation adjustment of pretreatment system and switching operation of bed were discussed.

对二级脱盐水系统中铁离子含量超标的原因，如来水水质发生波动、再生剂受到污染、预处理系统操作调整、床体运行切换等进行了论述。

You were hired to drum up new business, so go and do it.

公司雇你招徕新业务，你就做你的事好了。