挑出

To be, or not to be; that is the bare bodkin That makes calamity of so long life; For who would fardels bear, till Birnam Wood do come to Dunsinane, But that the fear of something after death Murders the innocent sleep, Great nature's second course, And makes us rather sling the arrows of outrageous fortune Than fly to others that we know not of.

活下去呢，还是不活下去，这是一把出鞘的宝剑，使这漫长的一生成为无穷的灾难，谁愿挑着重担，一直到勃南森林，真是来到了邓西宁，可是对死后的遭遇深怀恐惧，害死了无忧无虑的睡眠，伟大天性的第二条路，使我们宁愿抛出恶运的毒箭，决不逃往幽冥去寻求解脱，正是为了这个缘故我们才不得不踌躇。

The spear went through the shield and passed on through his richly wrought cuirass, tearing the flesh from his side, but Pallas Minerva did not suffer it to pierce the entrails of the hero.

言罢，他出枪击中俄底修斯身前溜圆的战盾，沉重的枪尖深扎进闪亮的盾面，挑开精工制作的胸甲，捅裂了肋骨边的皮肉；然而，帕拉丝·雅典娜不让枪尖触及他的要害。

If you have fine hair, you can try use lowlights to create the illusion of volume.

如果你的头发很幼的话，可以做发色挑染来造出丰满发型的效果。

RED 瑞德 Bear Catholic? Pope shit in the woods? S mokes or coin, bettor's choice.

拿出笔和本废话少说，勿庸多言，赌烟或者赌钱，自己挑。

At the worst you are being ungrateful turds because someone is giving you a piece of software, but you insult their work not on its technical merits, but on a license file you disagree with.

你从别人那里不费吹灰之力获得了有用的软件，你不但提不出任何技术性质的见解和批评，却单单拿一个授权来挑刺。

All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板，挑取其中的白斑经活化培养后，运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板)，即5，000 余个重组子克隆的高质量质粒DNA；提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction，聚合酶链式反应)扩增出重组子插入片段，标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5，000 余条5′端EST 序列的测定工作，初步筛选出4，747 条质量较好的EST序列，经筛选得到的EST中90%以上具有大于500bp 的可读序列，每一EST序列中不可读碱基数小于5%。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区，克隆进PET-32a表达载体，双酶切和PCR鉴定出阳性者，命名为PET-32pgE，转化BL21菌株，挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和，按2％体积转种另一管，培养至OD_(600)为0.6，加IPTG至终浓度1mmol／l，诱导4～6小时，SDS-PAGE和Westernblot分析，证明确实表达并具有免疫反应性。

This research is taking sea perch as the material, and cloning the Syntaxin 1B gene. After extracting RNA from sea perch tissue, we gained the first chain of cDNA by RT-PCR. Then designing a pair of primers according to the sequence of Syntaxin 1B from other organism. Based on the first chain of cDNA cloned from sea perch, the partial sequence of Syntaxin 1B gene is been cloned by using TD-PCR and we received the 751bp fragment at last.

本实验从鲈鱼脑组织中提取总RNA，通过反转录得到cDNA第一条链，根据已克隆出的其它生物的Syntaxin 1B基因序列，设计并合成引物，在妒鱼syntaxin1B基因部分序列克隆与分析 cDNA第一条链的基础上使用降落PCR，进行体外扩增妒鱼 Syntaxin IB基因的部分序列，得到长度为75 lbP的片断，在胶中洗脱下该片段，与pMD 18一T载体链接，转化入感受态细胞，挑取阳性克隆扩大培养，提取质粒，经HindIH和x一gaH两内切酶消化得到75 1 bp的片断，证明链接成功。

Unmoved by traffic, a female snapping turtle chooses a nest site beside a road…Before this June day ends, she will have dug a hole with her hind legs and deposited golf-sized eggs.

图中这头雌性鳄龟对来往车辆表现得无动于衷，坚持在路边挑了一块地方当做巢穴。。。在六月份结束之前，她将在此处用后腿挖出一个洞，然后放进她下的高尔夫球大小的蛋。

Once all entries are received, Glam's editorial team will select 4 groups of 8 semifinalists that will be featured in a weekly slideshow with voting functionality.

待收到所有草图后，Glam的编辑们会挑选出4组半决赛选手，每组为8人，并且每周都以专版刊登出这些选手的设计图，读者们可以在线投票。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。