挑中

A novel rapid alkalinization factor gene of litchi was isolated from cDNA library of litchi pericarpGenBank accession No.

从已构建的荔枝果皮cDNA文库中挑取克隆测序，获得一条荔枝快速碱化因子基因（GenBank登录号：EU024484）。

She showed her friends the stack of old love letters and read them excerpts from the couple's amatory correspondence.

她拿了一堆旧情书给朋友看，并挑了些信中的艳情部分读了出来。

The GongBuCi have to describe according to the poem of DuFu behind with the structure and form of Ming Dynasty" grass shed view area" of recovering the reconstruction, re-appearing the pastoral appearance that poet so reside, constructing the thick thick poetry atmosphere;Have to set up in 1999 in flower arrangement park of" DuFu poem calligraphy wood-carved", display more than 100 DuFu's poem calligraphy wood-carved work, choose from the building hides several thousand in the pasts a hand book poem genuine calligraphy, engrave with the wood but, have to appreciate the value rather, its verse, calligraphy, have with the material, craft" four unique" it call;" big the hall" inside that locate the important male in a the third in building in thou in peaceful temple in original treasure , display the up to the present the large colored enamel of the local and biggest area(64 square meter) insets to whet to paint the mural with 12 in the pasts anthologist the person carves, image a development for displaying Du Fu usually with Chinese classic verse history;Building inside of basic display the 《 poem saint a thousand years 》, have the honor of acquiring the fifth" whole country ten big display the exhibition exquisite article" the best creativity prize.

工部祠后有依据杜诗描写和明代格局恢复重建的"茅屋景区"，重现了诗人故居的田园风貌，营造出浓厚的诗意氛围；在盆景园内有1999年建成的"杜诗书法木刻廊"，陈列着百余件杜诗书法木刻作品，是从馆藏数千件历代名人手书杜诗真迹中挑选出，用楠木镌刻而成，颇具观赏价值，其诗歌、书法、用材、工艺有"四绝"之称；位于原梵安寺古建筑群第三重大雄宝殿的"大雅堂"内，陈列着迄今为止国内最大面积（64平方米）的大型彩釉镶嵌磨漆壁画和12尊历代著名诗人雕塑，形象地展示了杜甫生平和中国古典诗歌的发展史；馆内的基本陈列《诗圣著千秋》，荣获了第五届"全国十大陈列展览精品"最佳创意奖。

There are many holes that can be picked in such a weak argument.

在这样一篇无力的辩解中可以挑出很多漏洞。

There is little point to getting it heads up by raising with this hand, and the bettor is going to call you anyway.

在这个牌例中，如果是单挑，你不应该加注，最好的做法是无论如何都跟注。

Parlour games (dominos, halma, tiddledywinks, spilikins, cup and ball, nap, spoil five, bezique, twentyfive, beggar my neighbour, draughts, chess or backgammon): embroidery, darning or knitting for the policeaided clothing society: musical duets, mandoline and guitar, piano and flute, guitar and piano: legal scrivenery or envelope addressing: biweekly visits to variety entertainments: commercial activity as pleasantly commanding and pleasingly obeyed mistress proprietress in a cool dairy shop or warm cigar divan: the clandestine satisfaction of erotic irritation in masculine brothels, state inspected and medically controlled: social visits, at regular infrequent prevented intervals and with regular frequent preventive superintendence, to and from female acquaintances of recognised respectability in the vicinity: courses of evening instruction specially designed to render liberal instruction agreeable.

191室内游戏（多米诺骨牌，希腊跳棋，挑圆片），抽杆游戏，杯球，纳普，抢五墩牌，比齐克，二十五墩，"抢光我的邻居"，跳棋，国际象棋或十五子棋戏）；为警察署资助的服装协会做刺绣、缝补或编织等活计；音乐二重奏：曼陀林和吉他，钢琴和长笛，吉他和钢琴；法律文件的抄写或代填信封上的地址；每隔一周去看一次杂耍演出；从事一些商业活动：一位老板娘在凉爽的牛奶房或暖和的香烟店里愉快地使唤着，愉快地被服从着；在由国家监督、并加以医药管理的男妓院里，暗自从淫欲刺激中得到的满足；与住在附近的一些被公认为品行端正的女友们进行社交活动，需要有不频繁的定期预防性间隔以及频繁的定期预防性监督；为了讲授合适的交往礼仪而专门举办一套夜间讲座。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区，克隆进PET-32a表达载体，双酶切和PCR鉴定出阳性者，命名为PET-32pgE，转化BL21菌株，挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和，按2％体积转种另一管，培养至OD_(600)为0.6，加IPTG至终浓度1mmol／l，诱导4～6小时，SDS-PAGE和Westernblot分析，证明确实表达并具有免疫反应性。

A process for preparing the recombinant chicken gamma-interferon (rCHIFN-gamma) with high activirus activity includes such steps as cloning ChIFN-gamma gene from eukaryotic plasmid to transfer carrier by dual enzyme servenings to obtain recombinant transfer plasmid pFASTBACI-ChIFN-gamma, taking DH10Bac competent cell, adding IngpFASTBAC1-ChIFN-gamma, transposition, diluting to culture object by SOD culture medium, coating on Luria plate, culturing, choosing white clony, purifying, naming positive plasmid as Bacmid-ChIFN-gamma, extracting its DNA, and transfecting Sf9 cells.

高抗病毒活性重组鸡γ-干扰素的制取方法及用途，涉及一种广谱高效抗病毒基因工程产品的制取方法。将ChIFN-γ基因从真核质粒中经双酶切后克隆到转移载体中，获得重组转移质粒pFASTBAC1-ChIFN-γ；取DH10Bac感受态细胞，加入1ng pFASTBAC1-ChIFN-γ，转座后，用SOC培养基稀释培养物，涂布Luria平板，培养后，挑取白色菌落，Luria平板进行纯化，阳性质粒命名为Bacmid-ChIFN-γ，提取阳性质粒DNA；取上述重组DNA质粒转染Sf9细胞，制得高抗病毒活性重组鸡γ-干扰素。

This research is taking sea perch as the material, and cloning the Syntaxin 1B gene. After extracting RNA from sea perch tissue, we gained the first chain of cDNA by RT-PCR. Then designing a pair of primers according to the sequence of Syntaxin 1B from other organism. Based on the first chain of cDNA cloned from sea perch, the partial sequence of Syntaxin 1B gene is been cloned by using TD-PCR and we received the 751bp fragment at last.

本实验从鲈鱼脑组织中提取总RNA，通过反转录得到cDNA第一条链，根据已克隆出的其它生物的Syntaxin 1B基因序列，设计并合成引物，在妒鱼syntaxin1B基因部分序列克隆与分析 cDNA第一条链的基础上使用降落PCR，进行体外扩增妒鱼 Syntaxin IB基因的部分序列，得到长度为75 lbP的片断，在胶中洗脱下该片段，与pMD 18一T载体链接，转化入感受态细胞，挑取阳性克隆扩大培养，提取质粒，经HindIH和x一gaH两内切酶消化得到75 1 bp的片断，证明链接成功。

In an effort to face the challenges of life, we often turn to Solomon's words:"To everything there is a season, a time for every purpose under heaven" Eccl.

当我们面对生命中的挑战时，常从所罗门的话中寻求力量：「凡事都有定期，天下万务都有定时」（传道书3章1节）。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。