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C "Repick,"the fairy said, No doubt the time had taught you clear.

&重新吧,&仙女道,岁月无疑把你教聪明了。

Inoculate a single colony in BMGY medium, grow at 30 °C with 250 r/min until culture reatch an OD6oo=2.0. Harvest the cells by centrifuging at 1,500 r/min for 5 min at room temperature. Resuspend cell pellet to an OD6oo=10 in BMMY medium to start induction.

将经过酶切鉴定和测序正确的阳性克隆电转入毕赤酵母GS115中,取酵母转化单菌落,接种于BMGY培养基,30℃、250r/min培养至OD_(600)=2.0时室温1,500r/min离心5min,用BMMY培养基重悬菌体使OD_(600)=1.0,重新放入摇床进行诱导。

Hanne Roer, 30, was heading off to(17) a library to pick up a handful of(18) crime novels before leaving Oslo for Easter.

30岁的汉娜·罗尔将离开奥斯陆去过复活节,临行之前要去图书馆几本犯罪小说。

For instance, Chinese people are honest but slick, sincere but shrewd, skeptical but credulous, rigid but flexible, self-serving but loyal, sophisticated but inconsiderate, neutral but extreme, frugal but ostentatious, old-fashioned but fashionable, low maintenance but dreaming of fortune, superstitious but not religious, team-spirited but competitive, picky but mediating, rumorous but not nosy, understanding the value of time but advocating 'take things slowly', etc., etc..

比方说,中国人耿直却又圆滑,坦诚却又世故,多疑却又轻信,古板却又灵活,讲实惠却又重义气,尚礼仪却又少公德,主中庸却又走极端,美节俭却又喜排场,守古法却又赶时髦,知足常乐却又梦想暴发,烧香算命却又无宗教感,爱抱团儿却又好窝里斗,爱刺儿却又会打圆场,不爱管闲事却又爱说闲话,懂得&只争朝夕&的道理却又主张&慢慢来&,等等,等等。

Parlour games (dominos, halma, tiddledywinks, spilikins, cup and ball, nap, spoil five, bezique, twentyfive, beggar my neighbour, draughts, chess or backgammon): embroidery, darning or knitting for the policeaided clothing society: musical duets, mandoline and guitar, piano and flute, guitar and piano: legal scrivenery or envelope addressing: biweekly visits to variety entertainments: commercial activity as pleasantly commanding and pleasingly obeyed mistress proprietress in a cool dairy shop or warm cigar divan: the clandestine satisfaction of erotic irritation in masculine brothels, state inspected and medically controlled: social visits, at regular infrequent prevented intervals and with regular frequent preventive superintendence, to and from female acquaintances of recognised respectability in the vicinity: courses of evening instruction specially designed to render liberal instruction agreeable.

191室内游戏(多米诺骨牌,希腊跳棋,圆片),抽杆游戏,杯球,纳普,抢五墩牌,比齐克,二十五墩,&抢光我的邻居&,跳棋,国际象棋或十五子棋戏);为警察署资助的服装协会做刺绣、缝补或编织等活计;音乐二重奏:曼陀林和吉他,钢琴和长笛,吉他和钢琴;法律文件的抄写或代填信封上的地址;每隔一周去看一次杂耍演出;从事一些商业活动:一位老板娘在凉爽的牛奶房或暖和的香烟店里愉快地使唤着,愉快地被服从着;在由国家监督、并加以医药管理的男妓院里,暗自从淫欲刺激中得到的满足;与住在附近的一些被公认为品行端正的女友们进行社交活动,需要有不频繁的定期预防性间隔以及频繁的定期预防性监督;为了讲授合适的交往礼仪而专门举办一套夜间讲座。

With the release of their seventh album "Always And Forever", their debut release on Shanachie Entertainment on October 17th, Silk brings their vocal artistry to a hand-picked selection of songs that have inspired them as simultaneous homage to artists who they respect and musical statement of the ultimate quality of Silk's music.

十月17日发行的《Always And Forever》是他们的第十七张专辑,同时也是在Shanachie Entertainment公司发行的第一张专辑。在这张专辑里Silk通过精细选的唱曲表达了对所他们敬重的音乐家的无限敬意,同时也展现了Silk音乐之中的极致魅力。

Shita zuo under the brick, notxu mi zuo -style facilities, the selected canopies tower.

最下施砖砌塔座,不施须弥座式,上出塔檐。

There they found out why they were singled out of the crowd.

他们在那里发现了他们为什么被出来的人群。

What happened, appears to have gone something like this, for those that were singled out, that is.

发生了什麼,似乎已经经历了这样的,对於那些被出来,即是。

One reason may be singled out as one of the most prevalent.

原因之一可能是出一个最普遍。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。