挑

The fragment was connected to pcDNA3.0 carrier which was cut by the same two enzymes to form the plasmid which was transformed into Escherichia coli DH5a.Appropriate clone was cultured to extract plasmid.The extract was analysed to confirm that it was constructed correctively.

将该片段连接到同样被KpnⅠ和XhoⅠ双酶切的pcDNA3.0载体中，转化大肠杆菌DH5α感受态细菌，挑取阳性克隆进行质粒提取，进行酶切鉴定，并获得阳性克隆。

That's why I say that the Second Field Army shouldered a heavy load in the strategic counteroffensive, or as I like to say, we passed the test.

所以说，战略反攻，二野挑的是重担，还是那句老话，叫做合格。

From the lady's elflock hair, we can realize that she is a preferring foreign things person, but a real countrified lady. You will find that these two persons are just with similar faces and temperament. All the persons will trust they are the couple appearances.

用不着仔细看观者一定注意到他们相似的面孔和气质，说是夫妻相，谁都会相信，说是后由艺术家本人所扮，也极有可能，但这并不重要，因为作品的标题已经挑明是《一样的我和一样的你》。

This is a sin the countryfolk are reluctant to bear, so first they scrub them until they are sparklingly white and then they bring them into town.

这是一件罪过的事情，他们不愿意担在身上，故而先把它们濯得这样洁白了，才挑进城里来。

At home,on any early autumnmorning,many countryfolk would pass my door:the men with bronzedal'nls and protruding calf muscles,their bodies tall and uptight,evok-ing a sense of well-being;the women,usually with white turbans dot.ted with dark flowers,go barefoot and wear short coarse linen skirts,and while they ale not as tall as the men,they still have their ownbrand of healthy beauty.Each carries a shoulderpole with bundles fullof long,fresh and tender sections of lotus root the coour of whitejade.

若在故乡，每当新秋的早晨，门前经过许多乡人：男的紫赤的胳膊和小腿肌肉突起，躯干高大且挺直，使人起健康的感觉；女的往往裹着白地青花的头巾，虽然赤脚，却穿短短的夏布裙，躯干固然不及男的那样高，但是别有一种健康的美的风致；他们各挑着一副担子，盛着鲜嫩的玉色的长节的藕。

When facing opportunities and challenges ,we must courageously greet them with full preparation.

当面临机遇和挑战时，我们必须作好充分的准备勇敢地迎接他们。

Soon, four men in servant clothes walk out. Two carry empty shoulder pole, one brings the lantern and the other totes an oil jar. Doug pretends begging and falls down beside the guy who totes jar. She pastes the bag with cowskin glue on the inner side of lower hem of his clothes. That guy condemned Doug impatiently and kicked her then keeps going.

不久从后门走出四个穿著仆役服装的男子，两个挑著空担，一个拿著灯笼，另一个背著个油醰，豆豆假装乞讨，跌在背油醰之人身侧，把那上面有牛皮胶的小包黏在了那人下摆内侧，那人烦躁地骂了一句，踢了豆豆一脚，就继续往前走去了

I pick the crabs 3, 9, both about 30 yuan as long as my money.

我挑了3只螃蟹，9两左右，只要了我30元钱。

When you're ready for a real challenge, try out your French Cranium or Scrabble skills.

当你准备好面对真正的挑战时，你就可以试一下法国头盖骨游戏或者拼字游戏了。

Each generation of critics seems to select one particular usage to stand as the emblem of what they view as linguistic crassness.

每一代的批评家好象都挑了一个特别的用法作为他们认为的语言上的愚蠢行为的象征。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。