按蚊的

Gravid An. sinensis demonstrated significant negative taxis towards bacteria A4 and Aeromonas sp., as well as slight taxis to Enterobacter sp., implying that the three bacteria were not the dietary preference for An. sinensis. However, in light of the possibility of their stable colonization into mosquito's guts, these strains may serve as the host cells of larvicide genes and transgenic anopheline antimalarial genes.

中华按蚊产卵时对A4菌和气单胞菌具有明显的负趋性，而对肠杆菌仅具有微弱的趋向性，推测这3种细菌可能不是中华按蚊食物的优先选择，但具有在蚊中肠内较为稳定存活的可能性，因此更适合于作为蚊幼生物杀虫基因的寄主细胞和仿转基因按蚊抗疟基因的寄主细胞被应用。

Minimus density were collected from 1984 to 1993. Grey correlation analysis was carried out to study the relationship of 18 indices with An. minimus density. E variable was developed and its relation to the An. minimus density was analyzed to establish an evaluation model.

对18项指标与微小按蚊密度的关系进行灰色关联度分析，按照灰色阈值筛选评价指标，合成变量E，研究变量E与微小按蚊密度的关系，从而建立微小按蚊密度评价灰色模型。

In D3 gene with 5 site mutations. There exists individual variation in the COII gene, but this variation is less than that among other members of Myzomyia Series such as An. aconitus, An. jeyporiensis, etc. In addition, one haplotype different from both An. minimus A and C was found in these 3 genes studied.②The genetic distance between An. minimus A and An. minimus C is less than that among other members of Myzomyia Series. Molecular trees based on different genes and different methods show that An.

种系发生研究表明，微小按蚊复合体亲缘种A、C间的遗传距离远小于与之近缘的同系其它成员间的距离。2个分子标记3种方法构建的基因树显示，微小按蚊A、C的种系地位非常固定，不同地域来源、不同变异类型的微小按蚊始终在同一分"枝"上，与迈蚊系其它成员相比，呈现其相对稳固的单源性。

Sporozoite positive mosquitoes in the salivary glands were found from 1 010 parous mosquitoes in 7 Anopheles species in the fields;CSP were detected positive in 11 mosquitoes by ELISA out of 5 154 mosquitoes in 8 Anopheles species. sporozoites rate was 0.37％, 0.22％ and 0.32％ in Anopheles minimus, An.

现场显微镜解剖7种按蚊，1 010只经产蚊中，发现了7只唾液腺感染子孢子；ELISA方法检测现场捕回的8种按蚊，5 154只各龄期蚊中，发现11只环子孢子蛋白阳性蚊。

Objective To analyze the randomly amplified polymorphic DNA bands of Anopheles dirus and A.stephensi,and the relationship between the host genotype the susceptibility of anopheline mosquitoes to Plasmodium yoelii.

目的分析对约氏疟原虫不易感的大劣按蚊和易感的斯氏按蚊基因组RAPD谱带并测序，探讨媒介按蚊基因型与疟原虫基因型间的相互关系。

Results Compared with control areas,density of An anthropophagous deˉcreased by57.85%～100%and An sinensis decreased by27.06%～86.05%;the malaria incidence decreased by27.32%～100%.Cost calculation:the cost was0.59Yuan per person with deltamethrin bednet impregnation and1.17Yuan with deltamethrin impregnated bednets and alphamethrin residual spraying.

结果 实验区与对照区相比，嗜人按蚊密度下降57.85%～100%，中华按蚊密度下降27.06%～86.05%；疟疾发病下降27.32%～100%；以人均花费进行成本核算，溴氰菊酯浸帐灭蚊的费用为0.59元/人，溴氰菊酯浸帐加人群活动场所氯氰菊酯滞留喷洒灭蚊的费用为1.17元/人。

The melanotic phenomena mediated by PPO is widespread in both mosquitoes and other insects that being involved in sclerotization and canning of the egg shell and cuticle,wound healing and encapsulation of invading microorganisms.

目前，已从冈比亚按蚊克隆到6种不同的PPO基因，但斯氏按蚊与库态按蚊仅克隆到一种，并对其在按蚊抗疟原虫感染中的作用进行了初步研究。

In D3 gene with 5 site mutations. There exists individual variation in the COII gene, but this variation is less than that among other members of Myzomyia Series such as An. aconitus, An. jeyporiensis, etc. In addition, one haplotype different from both An. minimus A and C was found in these 3 genes studied.②The genetic distance between An. minimus A and An. minimus C is less than that among other members of Myzomyia Series. Molecular trees based on different genes and different methods show that An.

种系发生研究表明，微小按蚊复合体亲缘种A、C间的遗传距离远小于与之近缘的同系其它成员间的距离。2个分子标记3种方法构建的基因树显示，微小按蚊A、C的种系地位非常固定，不同地域来源、不同变异类型的微小按蚊始终在同一分&枝&上，与迈蚊系其它成员相比，呈现其相对稳固的单源性。

RESULT ①Two different ITS2 sequences (GenBank accession number: AF416783, AF416784) and D3 sequences (AF416782, AF415594), named as An. minimus A and An. minimus C, were detected in this study. They differed by 5. 8％ in the ITS2 region with 22 fixed nucleotide substitutions and indels, and by 1. 5％ in D3 gene with 5 site mutations. There exists individual variation in the COII gene, but this variation is less than that among other members of Myzomyia Series such as An.

结果 ①分别发现两种不同的ITS2序列（GeneBank登录号：AF416783，AF416784）和D3序列（GeneBank登录号：AF416782，AF425594），与微小按蚊A和C同源。A、C两者ITS2序列22个固定位点存在碱基置换和插入／缺失，序列差异为4.8％；D3基因存在5个固定点突变，突变率1.5％。mtDNA-COII基因存在个体差异，但远小于同系其它成员如乌头按蚊、杰普尔按蚊间的变异。

OBJECTIVE ①To compare the sequences of the ribosomal rDNA-ITS2, 28S-D3 and mitochondrial mtDNA-COII in the different origins of Anopheles minimus for detecting the intra-and inter-specific variation of the sibling species of the Minimus Complex;②To reconstruct phylogenetic trees of the Minimus Complex and Myzomyia Series for determining the position of An.

目的 ①比较微小按蚊核糖体rDNA-ITS2、28S-D3基因和线粒体mtDNA-COII基因的序列差异，阐明微小按蚊复合体亲缘种种间和种内变异；②根据基因变异重建微小按蚊复合体及迈蚊系成员的种系发生关系，以确立微小按蚊亲缘种变异的种系地位；③建立微小按蚊复合体亲缘种A和C的分子鉴别方法。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。