按蚊

Minimus density were collected from 1984 to 1993. Grey correlation analysis was carried out to study the relationship of 18 indices with An. minimus density. E variable was developed and its relation to the An. minimus density was analyzed to establish an evaluation model.

对18项指标与微小按蚊密度的关系进行灰色关联度分析，按照灰色阈值筛选评价指标，合成变量E，研究变量E与微小按蚊密度的关系，从而建立微小按蚊密度评价灰色模型。

In order to study the houses invading and community characteristics of An. minimus in different environment, the CDC traps were adopt to catch the mosquitoes in human houses.

：为了比较不同生态环境中微小按蚊入侵人房和不同生境微小按蚊群落学特征，本研究采用CDC诱蚊灯人房通宵捕蚊。

Sinensis and An. maculatus respectively (including CSP positive by ELISA and sporozoite positive in the salivary glands), and their entomological inoculation rate was estimated to be 9.82, 3.97 and 2.69 respectively.

微小按蚊、中华按蚊、多斑按蚊子孢子阳性率(包括ELISA环子孢子蛋白阳性和镜检唾腺子孢子感染蚊)分别是0.37％、0.22％、0.32％，昆虫接种率分别估算为9.82、3.97、2.69。

Maculatus respectively(including CSP positive by ELISA and sporozoite positive in the salivary glands),and their entomological inoculation rate was estimated to be 9.82,3.97 and 2.69 respectively.

微小按蚊、中华按蚊、多斑按蚊子孢子阳性率(包括ELISA环子孢子蛋白阳性和镜检唾腺子孢子感染蚊)分别是0.37%、0.22%、0.32%，昆虫接种率分别估算为9.82、3.97、2.69。

Objective To analyze the randomly amplified polymorphic DNA bands of Anopheles dirus and A.stephensi,and the relationship between the host genotype the susceptibility of anopheline mosquitoes to Plasmodium yoelii.

目的分析对约氏疟原虫不易感的大劣按蚊和易感的斯氏按蚊基因组RAPD谱带并测序，探讨媒介按蚊基因型与疟原虫基因型间的相互关系。

Methods With deltamethrin impregnated bednets and alphamethrin residual spraying and bednet impregnation to control malaria vectors,observe the change of density of An anthropophagous and Ansinensis and malaria incidence and calculate the costs.

采用溴氰菊酯浸帐灭蚊和浸帐加氯氰菊酯滞留喷洒的方法防制疟疾媒介，观察嗜人按蚊、中华按蚊的密度变化和疟疾发病率，按照人均所需费用核算成本。

Results Compared with control areas,density of An anthropophagous deˉcreased by57.85%～100%and An sinensis decreased by27.06%～86.05%;the malaria incidence decreased by27.32%～100%.Cost calculation:the cost was0.59Yuan per person with deltamethrin bednet impregnation and1.17Yuan with deltamethrin impregnated bednets and alphamethrin residual spraying.

结果 实验区与对照区相比，嗜人按蚊密度下降57.85%～100%，中华按蚊密度下降27.06%～86.05%；疟疾发病下降27.32%～100%；以人均花费进行成本核算，溴氰菊酯浸帐灭蚊的费用为0.59元/人，溴氰菊酯浸帐加人群活动场所氯氰菊酯滞留喷洒灭蚊的费用为1.17元/人。

The melanotic encapsulation of oocysts is a specific immune defence mechanism against Plasmodium infection ,and is also a primary phenotype in the Plasmodium-refractory strain of An.gambiae.

卵囊黑化包被反应是按蚊抵御疟原虫感染的特异性免疫防御机制，也是抗性株冈比亚按蚊或不易感按蚊的最主要表型。

RESULT ①Two different ITS2 sequences (GenBank accession number: AF416783, AF416784) and D3 sequences (AF416782, AF415594), named as An. minimus A and An. minimus C, were detected in this study. They differed by 5. 8％ in the ITS2 region with 22 fixed nucleotide substitutions and indels, and by 1. 5％ in D3 gene with 5 site mutations. There exists individual variation in the COII gene, but this variation is less than that among other members of Myzomyia Series such as An.

结果 ①分别发现两种不同的ITS2序列（GeneBank登录号：AF416783，AF416784）和D3序列（GeneBank登录号：AF416782，AF425594），与微小按蚊A和C同源。A、C两者ITS2序列22个固定位点存在碱基置换和插入／缺失，序列差异为4.8％；D3基因存在5个固定点突变，突变率1.5％。mtDNA-COII基因存在个体差异，但远小于同系其它成员如乌头按蚊、杰普尔按蚊间的变异。

Objective To determine the oviposition responses of Anopheles sinensis to bacteria A4, Aeromonas sp. and Enterobacter sp. and whether or not the three strains attracted the mosquitoes, providing theoretical and experimental evidence for the application of the three bacteria in the intervention of malaria. Methods Blood?fed and held for 48-60 h, copulated female An.

目的 具吸引作用的细菌或其挥发物能提高按蚊与杀虫或抗疟工程细菌接触的机会，为此，拟测定中华按蚊对A4细菌、气单胞菌和肠杆菌的产卵反应特性，以明确3种细菌对中华按蚊是否具有吸引特性，为应用这3种细菌开展阻断疟疾传播研究提供理论和实验依据。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。