按蚊

The morphological and molecular variations between AK and AL were small and belong to intraspecific level.

两种按蚊形态特征和分子序列差异极小，应属种内变异范围，可以确认两者是同一蚊种，昆明按蚊为凉山按蚊的同物异名。

Gravid An. sinensis demonstrated significant negative taxis towards bacteria A4 and Aeromonas sp., as well as slight taxis to Enterobacter sp., implying that the three bacteria were not the dietary preference for An. sinensis. However, in light of the possibility of their stable colonization into mosquito's guts, these strains may serve as the host cells of larvicide genes and transgenic anopheline antimalarial genes.

中华按蚊产卵时对A4菌和气单胞菌具有明显的负趋性，而对肠杆菌仅具有微弱的趋向性，推测这3种细菌可能不是中华按蚊食物的优先选择，但具有在蚊中肠内较为稳定存活的可能性，因此更适合于作为蚊幼生物杀虫基因的寄主细胞和仿转基因按蚊抗疟基因的寄主细胞被应用。

In D3 gene with 5 site mutations. There exists individual variation in the COII gene, but this variation is less than that among other members of Myzomyia Series such as An. aconitus, An. jeyporiensis, etc. In addition, one haplotype different from both An. minimus A and C was found in these 3 genes studied.②The genetic distance between An. minimus A and An. minimus C is less than that among other members of Myzomyia Series. Molecular trees based on different genes and different methods show that An.

种系发生研究表明，微小按蚊复合体亲缘种A、C间的遗传距离远小于与之近缘的同系其它成员间的距离。2个分子标记3种方法构建的基因树显示，微小按蚊A、C的种系地位非常固定，不同地域来源、不同变异类型的微小按蚊始终在同一分"枝"上，与迈蚊系其它成员相比，呈现其相对稳固的单源性。

Sporozoite positive mosquitoes in the salivary glands were found from 1 010 parous mosquitoes in 7 Anopheles species in the fields;CSP were detected positive in 11 mosquitoes by ELISA out of 5 154 mosquitoes in 8 Anopheles species. sporozoites rate was 0.37％, 0.22％ and 0.32％ in Anopheles minimus, An.

现场显微镜解剖7种按蚊，1 010只经产蚊中，发现了7只唾液腺感染子孢子；ELISA方法检测现场捕回的8种按蚊，5 154只各龄期蚊中，发现11只环子孢子蛋白阳性蚊。

The melanotic phenomena mediated by PPO is widespread in both mosquitoes and other insects that being involved in sclerotization and canning of the egg shell and cuticle,wound healing and encapsulation of invading microorganisms.

目前，已从冈比亚按蚊克隆到6种不同的PPO基因，但斯氏按蚊与库态按蚊仅克隆到一种，并对其在按蚊抗疟原虫感染中的作用进行了初步研究。

In D3 gene with 5 site mutations. There exists individual variation in the COII gene, but this variation is less than that among other members of Myzomyia Series such as An. aconitus, An. jeyporiensis, etc. In addition, one haplotype different from both An. minimus A and C was found in these 3 genes studied.②The genetic distance between An. minimus A and An. minimus C is less than that among other members of Myzomyia Series. Molecular trees based on different genes and different methods show that An.

种系发生研究表明，微小按蚊复合体亲缘种A、C间的遗传距离远小于与之近缘的同系其它成员间的距离。2个分子标记3种方法构建的基因树显示，微小按蚊A、C的种系地位非常固定，不同地域来源、不同变异类型的微小按蚊始终在同一分&枝&上，与迈蚊系其它成员相比，呈现其相对稳固的单源性。

OBJECTIVE ①To compare the sequences of the ribosomal rDNA-ITS2, 28S-D3 and mitochondrial mtDNA-COII in the different origins of Anopheles minimus for detecting the intra-and inter-specific variation of the sibling species of the Minimus Complex;②To reconstruct phylogenetic trees of the Minimus Complex and Myzomyia Series for determining the position of An.

目的 ①比较微小按蚊核糖体rDNA-ITS2、28S-D3基因和线粒体mtDNA-COII基因的序列差异，阐明微小按蚊复合体亲缘种种间和种内变异；②根据基因变异重建微小按蚊复合体及迈蚊系成员的种系发生关系，以确立微小按蚊亲缘种变异的种系地位；③建立微小按蚊复合体亲缘种A和C的分子鉴别方法。

OBJECTIVE ①To compare the sequences of the ribosomal rDNA-ITS2, 28S-D3 and mitochondrial mtDNA-COII in the different origins of Anopheles minimus for detecting the intra-and inter-specific variation of the sibling species of the Minimus Complex;②To reconstruct phylogenetic trees of the Minimus Complex and Myzomyia Series for determining the position of An. minimus A and C;③To establish specific methods for differentiation of two sibling species, An. minimus A and An. minimus C, of the Minimus Complex.

中文题名微小按蚊种群变异与分子鉴别研究副题名外文题名论文作者周水森导师汤林华研究员学科专业分子流行病学研究领域\研究方向学位级别博士学位授予单位中国预防医学科学院学位授予日期2002 论文页码总数98页关键词蚊微小按蚊馆藏号BSLW /2003 /R384 /3 目的①比较微小按蚊核糖体rDNA-ITS2、28S-D3基因和线粒体mtDNA-COII基因的序列差异，阐明微小按蚊复合体亲缘种种间和种内变异；②根据基因变异重建微小按蚊复合体及迈蚊系成员的种系发生关系，以确立微小按蚊亲缘种变异的种系地位；③建立微小按蚊复合体亲缘种A和C的分子鉴别方法。

It's breeding and reproduction were remarkably affected by rainy season.There was no notable difference on the density and the parous rate between An.Dirus in the village where the nets were impregnated with deltamethrin and that in the control village.

山寮叮人率是村内叮人率的15倍，自然感染率可高达8.3%，雨季对大劣按蚊的孳生繁殖有极大的影响，溴氰菊酯浸帐村与对照村的大劣按蚊密度和经产蚊比率无显著性差异，在当地多种因素影响下，溴氰菊酯浸帐防制大劣按蚊的效果不理想。

Methods: Anopheles in house and mosquito net was catched with a catching mosquito tube in the region from An-anthropophagus' village to depth area of Jianghan plain.

以浅丘平原结合部嗜人按蚊村点为起点向江汉平原腹地垂直定点、定距离调查，捕捉人房及帐内按蚊，按照蚊卵及成蚊形态鉴定蚊种。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。