按技术

Communication with supplier for tech detail and define the tech agreement ,ensure the delivery timely and care about sign-off

负责与供应商协商设备技术细节和制定外委技术协议，跟踪供应商制造进度，保证及时交货，并最终按要求验收。

Main business: CNC engraving machine Series: Applications: stainless steel, copper engraving Case insets processing; stainless steel, copper Case Shouban; spherical, Globoidal processing, text, sculpture ★ Case 5-axis double-headed insets engraving machine ★ Case 5-axis three insets engraving machine ★ Case 4-axis linkage double-headed insets engraving machine ★ Case 4-axis linkage three insets engraving machine ★ high triaxial strong double-headed die machine ★ high strength triaxial 3 Die machine ★ Case Drilling Machine, surface drilling machine ★ Case-meter pit, watches and clocks, glass-processing machine production testing of aircraft series:● test the waters Series: 10 automatic machine manually test the waters, six automatic Manual examination of water, really test the water leak-loaded needle ● Series: Semi-automatic assembly line of watches and clocks, watches and clocks, with three-pin CNC machines, semi-automatic machine ● single needle assembly Series: Semi-automatic cutting the core of machines, Case glass adhesive glue Machines, pneumatic pressure Digai ● strap-test: strap Rally torque testing machine, clasp switch testing machine, strap swing testing machine ● first test table: Table heading to the functional testing of aircraft, form the first vibration test machines, Table first parabolic dynamic testing machine has been with the German company Rexroth, Japan Yasukawa, Panasonic, Mitsubishi, and other well-known international companies have a long-term stability of technical cooperation, continue to study and absorb the advanced technology at home and abroad.

主营业务： CNC雕刻加工机系列：应用：不锈钢、铜表壳镶石雕刻加工；不锈钢、铜表壳手板；球面、弧面加工、文字雕刻★表壳五轴联动双头镶石雕刻机★表壳五轴联动三头镶石雕刻机★表壳四轴联动双头镶石雕刻机★表壳四轴联动三头镶石雕刻机★高强力三轴双头模具加工机★高强力三轴三头模具加工机★表壳钻孔机、表面钻孔机★表壳米坑位、玻璃位加工机钟表生产测试机系列：●试水系列：十头自动手动试水机、六头自动手动试水机、真水试漏机●装针系列：半自动钟表装配生产线、数控钟表装三针机、半自动单针机●装配系列：半自动切把芯上的机、表壳玻璃胶水粘接机、气动压底盖机●表带测试：表带拉力扭力测试机、表扣开关测试机、表带摇摆测试机●表头测试：表头按的功能测试机、表头震动测试机、表头抛动测试机一直以来，公司与德国Rexroth、日本安川、松下、三菱等国际知名企业有着长期稳定的技术合作，不断地学习和吸收国内外的先进技术。

In teaching the content including three-view drawing and axonometric drawing in Technology and Design Book one which is published by Suzhou's Education Press, firstly the writer has made a adjustment on the order of the teaching content and a complementarity on the concept of the proportion of the three-view drawing.

执教《技术与设计1》三视图和轴测图的绘制教学内容，在教学实施中，对教学内容知识顺序作以调整，补充三视图比例概念，使用平面结构形体、曲面结构形体、组合结构形体模型实测画三视图；轴测图内容补充斜二测轴测图的绘制，使学生掌握识读工程图的基本方法、培养绘制工程三视图的基本能力、了解工程图的基本规范，初步养成按标准绘制工程图的良好习惯，挖掘学生对技术设计的内核的深刻理解和自身的原创力。

The Langmuir Blodgett technique was employed to modify the PVC liquid membrane, the LB film of trioctylcetyl ammonium chloroauric acid was deposited on the surface of PVC liquid membrane, and the gold selective electrode with such modification has been fabricated.

1引百 LB膜技术〔''是近年来超分子组装化学中应用最多和相对最成功的分子装配技术，因能较好地在许多固体表面按设计方式组装空间有序的超分子体系，特别是其器件研制最基本的实验手段之一卿，由于LB膜修饰层有序，高密，超薄，能从根本上改进电极体系及其反应过程的性质和行为

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Utilize the technology of NURBS surface fitting, to sort the product appearance form, and then to reconstruct it. Through experiment, verify the result of using NURBS surface fitting technology to reconduct free surface can get the satisfactory product form without the need for high precision conditions.

利用NURBS由面拟合技术，将产品外观按形态先进行分类，再据此对产品外形进行重构通过实验，验证了在不需要较高精度的条件下，利用NURBS曲面拟合技术对自由曲面进行重构，能获得令人满意的产品形态。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Each male ratwas paired with 2 female rats in proestrus when administration terminated. Femalerats were examined the next morning for the presence of sperm in vaginal smears andunderwent a cesarean section on day 13 of gestation. Then the reproductive indiceswere calculated as follows: copulation index, pregnancy index, and fertility index,which can evaluate male fertility directly. The sperm motility and morphology wereevaluated by computer-assisted sperm analysis, moreover, the caudaepididymal sperm ultramicrostructure were analyzed by transmission electronmicroscope, which can evaluate the influence on sperm maturation bydutasteride ; sperm survival rate was assessed by SYBR-14 and propidium iodidefluorescent staining; serumal dihydrotestosterone and testosterone of ratswere detected by enzyme-labeled immunoassay, which can evaluate the effecton androgenic levels; the weights of testes and epididymides, were determined byprecise electronic balance; histological examination of above tissues were evaluatedby HE staining and TEM, which can evaluate treatment on histology of reproductiveorgans by dutasteride.

给药结束后雄鼠与处于动情前期的雌鼠按2：1合笼，计算雌鼠的交配指数、受孕指数和生育指数，直接评价雄鼠生育力；采用计算机辅助精子分析系统分析大鼠附睾精子活力和形态，在此基础上合并使用透射电镜技术对各组附睾尾部精子进行透射电镜分析，评价度他雄胺对大鼠精子成熟的影响；采用SYBR-14和propidium iodide双重荧光染色计算精子存活率；采用Elisa法测定大鼠睾酮(testosterone， T)和双氢睾酮(dihydrotestosterone， DHT)血清浓度，评价度他雄胺对雄鼠雄激素水平的影响；采用精密电子天平对各组睾丸、附睾、前列腺和精囊进行称重，评价度他雄胺对大鼠生殖器官重量的影响；采用HE染色法对各组睾丸和附睾进行组织学分析，同时采用透射电镜技术对附睾上皮细胞超微结构进行分析，评价度他雄胺对大鼠主要生殖器官组织学的影响。

Methods Sixty patients with pulpitis or periapical periodontitis were divided into two groups randomly, which were obturated by continuous wave of condensation technique and lateral condensation technique respectively.

选择牙髓病或根尖周病患者60例，按就诊顺序随机分为两组，分别采用连续波充填技术和冷牙胶侧方加压充填技术进行根管充填。

In the "all for the sake of customers," the principle of qi in the first operation and management, has always been permeated with "good quality services for the surprising first" concept, built a strong technical services team, all received, such as education or The special factory training, technical professional staff walk the road, according to aircraft of the strict division of labor, thus raising professional standards to ensure quality of service, aimed at pre-sale, sale, service and strive to best meet customer requirements, the solution Users to worry about, so that users do not purchase five years behind, 10 years no regrets.

本着&一切为客户着想&的原则，奇先在经营和管理中，始终贯穿着&品质佳奇服务为先&的理念，建立了一支技术过硬的服务团队，全部受过中、等教育或制造厂的专项培训，技术人员走专业化道路，按机种严格分工，由此提高专业水平，保证服务质量，力求在售前、售中、售后服务努力做到最好，满足客户要求，解决用户的后顾之忧，使用户购机五年不落后，十年不后悔。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。