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The company that worked closely with the War Department of England to develop this new method of joining pipe was named Victory Pipe Joint Company after the coupling it had engineered — known as the "victory joint" from early military uses.

当时,与英格兰战争部密切合作以开发这一新的管道连接方法的公司按其设计制造出来的接头—因其早期军事用途被称为&胜利接头&—命名为胜利管道连接公司。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区,克隆进PET-32a表达载体,双酶切和PCR鉴定出阳性者,命名为PET-32pgE,转化BL21菌株,挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和,按2%体积转种另一管,培养至OD_(600)为0.6,加IPTG至终浓度1mmol/l,诱导4~6小时,SDS-PAGE和Westernblot分析,证明确实表达并具有免疫反应性。

Venetia, on the fateful day that Pluto popped into her head, was having breakfast with her mother and her gradfather, Falconer Madan, retired librarian of the Bodleian Library at Oxford. He had exciting news to tell.

在Pluto按她命名这个重要日子这天,Venetia正在与母亲和祖父Falconer Madan(牛津大学图书馆退休管理员一起吃早饭,他有一个振奋人心的消息要说。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。