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The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

对软骨细胞退变老化进行观察,采用相差显微镜观察其生长情况,透射电镜观察细胞结构,阿力新蓝染色检测胞外基质硫酸GAG含量和结构,免疫细胞化学法和RT-PCR方法检测Ⅱ型胶原鉴定软骨细胞,以MTT比色法描绘生长曲线、检测生长状态,组化法检测老化相关β-半乳糖苷酶,流式细胞仪分析细胞周期和增殖指数。3。

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养,同时以P2代软骨细胞为对照组,进行组化检测老化相关β-半乳糖苷酶,阿力新蓝染色检测胞外基质硫酸GAG含量和结构,MTT比色检测增殖,免疫细胞化检测PCNA和Ⅱ型胶原,RT-PCR检测Ⅱ型胶原、Aggrecan蛋白,流式细胞仪分析细胞周期和增殖指数等方法,对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养,同时以P2代软骨细胞为对照组,进行组化检测老化相关β-半乳糖苷酶,阿力新蓝染色检测胞外基质硫酸GAG含量和结构,MTT比色检测增殖,免疫细胞化检测PCNA和Ⅱ型胶原,RT-PCR检测Ⅱ型胶原、Aggrecan蛋白,流式细胞仪分析细胞周期和增殖指数等方法,对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

For the FrMVDR algorithm,the characteristic exponent value of the SαS clutter is first estimated,and then the fractional lower order output power of MIMO...

为了避免Fr MVDR算法对杂波特征指数估计,提出Inf-MVDR算法,首先用无穷范数对接收信号进行归一化处理,使归一化后的阵列输出功率有界,继而采用传统MVDR算法进行DOA估计。

Traditional method to coarsen and disperse the spatial data to lower administer unit in order to let spatial data be agreement to statistic data loses some spatial information. The comprehensive indexes got from these data of course cannot express the land-use spatial differentiation regularity well.

传统的研究方法是将空间数据"粗化"和"离散化"到行政单元上,与统计数据匹配,结果失去了一些指标的空间分布信息,得到的综合评价指数并不能较好地反映区域土地利用的空间分异规律。

The liver fibrotic model was induced by CCl4 i.p for 5wk. Telmisartan was given at the same time model establishment in each intervened group by administration lasted for 6wk. Decollate to get the blood of all rats after 7 weeks . ALT、AST and ALB of serum was detected by automatic biochemistry equipment. Hyaluronic acid was detected by ELISA detection. Liver and spleen was obtained to calculate the index. The liver pathological changes were observed in each group of rats after HE staining to evaluate the therapeutic effect of Telmisartan; TGF-β1、PPAR-γand PDGF of liver tissue was detected by the method of immunohistochemistry respectively; The mRNA expression of TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γand iNOS was detected by RT-PCR detection, respectively.

腹腔注射CCl4制备SD大鼠肝纤维化动物模型,连续灌胃6周,处死动物取血,检测血清ALT、AST、ALB;ELISA法测定血清透明质酸;取肝、睥,并计算肝、脾指数,组织切片HE染色后进行组织病理学疗效评估;用SP免疫组化法测定肝组织TGF-β1、PPAR-γ及PDGF蛋白表达;半定量RT-PCR方法检测肝组织TGF-β1、MMP-1、TIMP-1、α-SMA、PPAR-γ和iNOSmRNA的表达。

The mass loss patterns of four type litters fitted exponential model, and the decomposition rate could be well predicted by their lignin/N ratio.

结果表明:与对照相比,降水量减少条件下,蒙古栎叶凋落物的初始N、P、K浓度显著升高,初始木质素浓度显著降低,凋落物分解速率大,N、P、K矿化率高,N和P固持时间缩短;降水量增加情况下,其凋落物初始N浓度显著降低、木质素浓度显著升高,N、P、K矿化率低,N和P固持时间延长。4种类型叶片凋落物的质量损失过程均符合指数降解模型,分解速率可以由凋落物木质素/N来预测。

The role of six types of urban plantations in Harbin in maintaining woody plant diversity was assessed quantitatively by sample plot survey and diversity index analysis.

采用样地网格调查方法与多样性指数分析方法,定量评价了哈尔滨城市人工林在维持木本植物多样性方面的作用,并通过分析林分更新层、演替层、主林层的多样性指数,探讨了城市人工林自然化过程及其近自然经营途径。

The role of six types of urban plantations in Harbin in maintaining woody plant diversity was assessed quantitatively by sample plot survey and diversity index analysis. The naturalization process and the approaching-nature management modes of the urban plantations were also discussed in terms of richness, evenness and Shannon-Wiener indexes of regeneration layer, succession layer, main storey, brush layer aiming to provide a basis for forest management and biodiversity protection.

采用样地网格调查方法与多样性指数分析方法,定量评价了哈尔滨城市人工林在维持木本植物多样性方面的作用,并通过分析林分更新层、演替层、主林层的多样性指数,探讨了城市人工林自然化过程及其近自然经营途径,以便为哈尔滨城市森林经营与生物多样性保护提供依据。

There might be more than one Depth/Stencil format in the list,so to support changing from, say D3DFMT_D16 at index 0 to D3DFMT_D24X8 at index 2, the class keeps track of the Depth/Stencil format index, in the nDSFormat UINT, initialised by the settings constructor to 0, and changed via a SetDSFormat, with just a range check

可能有一个以上的深度/模板列表中的格式,所以,以支持不断变化的,说D3DFMT_D16索引为0的指数2 D3DFMT_D24X8,阶级不断的深度跟踪/模版格式的指数,在nDSFormat机组,由初始化设置构造为0,改变通过SetDSFormat,只需范围检查

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?