拷贝

Mouse genotype and C.rat genotype respectively,by RFLP and gene sequencing technique.The extracting effect of the imported commercial DNA extraction kit was the best,and the copies of COWP gene were(6.450-9.861 )×10~6 in the extraction.

Real-time PCR技术测定不同浓度NTZ和GAs作用后COWP基因拷贝数，透射电镜观察NTZ和GAs对虫体生长的影响。

The Promega commercial DNA extraction kit was used as control. Results The Promega kit resulted in a higher copy number of COWP gene[（6.45-9.86）×106]than that of Generay commercial DNA kit [（2.38-3.69）×106 ], 5% guanidine thiocyanate[（1.27-21.29）×105] or 2% Triton X-100 [（2.06-866.70）×103], respectively.

结果 Promega试剂盒的裂解液裂解隐孢子虫卵囊的效果最好，纯化所得卵囊COWP基因拷贝数可达（6.45～9.86）×106；其他依次为捷瑞试剂盒的裂解液[（2.38～3.69）×106]、5%异硫氰酸胍[（1.27～21.29）×105]和2% Triton X-100[（2.06～866.70）×103]。

I have seen some really great 'about me ' pages that are set up like sales copy websites and then I've seen some really cruddy ones or a lot that have none at all .

我已经看到了一些真正伟大的'我'的页面是成立一样，销售拷贝的网站，然后我看到了一些真正 cruddy 还是很多都没有在所有。

Having met some twenty-five thousand analysts, designers, and programmers throughout the world, I found a surprising number of them have never read any computer articles or evend a copy of Datamation or Computerworld; have never heard of ACM, DPMA, IEEE, ASM, or any other professional organizations; can't spell Dijkstra's name and probably have never heard of him; aren't aware of the structured techniques and wouldn't be interested if somebody showed them.

已经遇到过整个世界的25000个分析员、设计员及程序，我很吃惊的发现他们中的很多从来没有读过任何计算机的文章甚至是公开的Datamation或 computerworld的拷贝；没有听说过ACM，DPMA，IEEE，ASM，或者是其它任何专业组织；不能拼出Dijkstra的名字，可能从来没有听说过他；不清楚结构化技术，即使是有人告诉他们也不感兴趣。

Having met some twenty-five thousand analysts, designers, and programmers throughout the world, I found a surprising number of them have never read any computer articles or even opened a copy of Datamation or Computerworld; have never heard of ACM, DPMA, IEEE, ASM, or any other professional organizations; can't spell Dijkstra's name and probably have never heard of him; aren't aware of the structured techniques and wouldn't be interested if somebody showed them.

已经遇到过整个世界的25000个分析员、设计员及程序员，我很吃惊的发现他们中的很多从来没有读过任何计算机的文章甚至是公开的Datamation或 computerworld的拷贝；没有听说过ACM，DPMA，IEEE，ASM，或者是其它任何专业组织；不能拼出Dijkstra的名字，可能从来没有听说过他；不清楚结构化技术，即使是有人告诉他们也不感兴趣。

Having met some twenty-five thousand analysts, designers, and programmers throughout the world, I found a surprising number of them have never read any computer articles or even opened a copy of Datamation or Computerworld; have never heard of ACM, DPMA, IEEE, ASM, or any other professional organizations; can't spell Dijkstra's name and probably have never heard of him; aren't aware of the structured techniques and wouldn't be interested if somebody showed them.

已经遇到过整个世界的25000个分析员、设计员及程序，我很吃惊的发现他们中的很多从来没有读过任何计算机的文章甚至是公开的Datamation或 computerworld的拷贝；没有听说过ACM，DPMA，IEEE，ASM，或者是其它任何专业组织；不能拼出Dijkstra的名字，可能从来没有听说过他；不清楚结构化技术，即使是有人告诉他们也不感兴趣。

They think like clerks, talk like clerks, and they approach computer programming and systems analysis with all the enthusiasm of a sleepy civil service clerk who knows that hes just one year away from retirement.having met some twenty-five thousand analysts, designers, and programmers throughout the world, i found a surprising number of them have never read any computer articles or even opened a copy of datamation or computerworld; have never heard of acm, dpma, ieee, asm, or any other professional organizations; cant spell dijkstras name and probably have never heard of him; arent aware of the structured techniques and wouldnt be interested if somebody showed them.

他们象文职人员一样思考、交谈，他们与所有积极的，但昏昏欲睡的知道自己还有一年就退休的文职服务人员一起处理计算机编程和系统分析。已经遇到过整个世界的25000个分析员、设计员及程序，我很吃惊的发现他们中的很多从来没有读过任何计算机的文章甚至是公开的datamation或 computerworld的拷贝；没有听说过acm，dpma，ieee，asm，或者是其它任何专业组织；不能拼出dijkstra的名字，可能从来没有听说过他；不清楚结构化技术，即使是有人告诉他们也不感兴趣。

Havin g met some twenty-five thousan d analysts, designers, and pro grammers throughout the world, I found a surprising number o f them have never read any com puter articles or even opened a copy of Datamation or Comput erworld;have never heard of A CM, DPMA, IEEE, ASM, or any ot her professional organizations ;can't spell Dijkstra's name and probably have never heard of him;aren't aware of the st ructured techniques and wouldn 't be interested if somebody s howed them.

已经遇到过整个世界的25000个分析员、设计员及程序，我很吃惊的发现他们中的很多从来没有读过任何计算机的文章甚至是公开的Datamation或 comput erworld的拷贝；没有听说过ACM，DPMA，IEEE， ASM，或者是其它任何专业组织；不能拼出Dijkstra的名字，可能从来没有听说过他；不清楚结构化技术，即使是有人告诉他们也不感兴趣。

Screening and identification: the resulting plasmid was extracted by alkali division, identified by restriction enzyme digestions and PCR, observed by 1% agarose gels electrophoresis.

为了提高了目的片段和载体之间的连接效率以及目的片段的拷贝数，本实验所选用的载体是pMD18-T Vector，它是由pUC18用EcoRⅤ切割后在两个3'末端加上单个的碱基'T'而制得的线状分子。

Physical copy protection involves a physical property of the medium or requires a specific piece of hardware attached to a user's computer. The examples are hardware dongles attached to a serial or parallel port, and USB and smart card devices later.

物理拷贝保护涉及到介质的物理特性或者需要给用户的计算机加装一个特别的硬件，例如接在串口或并口的硬件保密狗，以及后来的USB和智能卡装置。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。