拷贝

The research demonstrated that the sperm-mediated gene transfer method (direct injection of copulation thecae) can introduce foreign gene into silkworms, and that the foreign gene may be integrated in the chromosomes of silkworms with many copies in series and can be transmitted to G1 offspring.

本研究的结果证明了精于介导基因转移方法能将外源基因有效地导入并整合于家蚕基因组，导入的外源基因可能是以串联多拷贝的形式整合于家蚕基因组中，整合的外源基囚叶＠传午G；代。

Reactive oxygen species causing DNA oxidative damage comes from two kinds of ways:one is from cellular normal physiological metabolism;the other is from outer environment.Redox-sensitive green fluorescent protein was expressed in Saccharomyces cerevisiae.Recombinant cells were evaluated in monitoring the changes in the redox state of living cells when challenged with toxicologically relevant metal ions NaAsO_2 or Pb(NO_3)_2 by measuring emission intensity at 510 nm with a Hitachi F6500 fluorescence spectrophotometer,roGFP expressed in yeast responded not only to typical membrane-permeant oxidants H_2O_2 and reductants DTT,but also to toxicological metal ion-induced intracellular redox changes in a dose-dependent manner.Moreover,exposure of yeast cells to NaAsO_2 or Pb(NO_3)_2 at concentrations that induced redox changes reported by roGFP caused up to 2～3 fold increases in DNA mutation frequency.This mutagenic effect was largely caused by oxidative stress since blocking the production of hydryl radicals with thiourea significantly reduced the mutation rate as well as delayed the cell death.

本文将对氧化还原状态变化敏感的绿色荧光蛋白roGFP1-R12，在酵母细胞中实现了多拷贝强表达；荧光扫描经强氧化剂H_2O_2和还原剂DTT以及环境中重金属NaAsO_2或Pb(NO_3)_2处理后的酵母细胞悬液，测定510 nm处的荧光发射强度结果显示，表达的绿色荧光蛋白对氧化还原水平敏感，且在510 nm处的荧光强度与一定的重金属浓度呈正相关，即roGFP1-R12在510nm处的荧光发射值随重金属浓度的增高而增强，从而说明重金属对细胞的毒性在一定程度上很可能是通过破坏细胞内的氧化还原平衡发生作用；同时通过该绿色荧光蛋白对胞内氧化还原状态变化的响应情况可以来实时检测环境中的重金属；遗传学的点突变频率及致死率实验数据表明，重金属能导致菌体的点突变频率和致死率升高，且活性氧的清除剂巯基脲能明显降低这种点突变和致死率，说明由重金属引发的这种点突变和致死效应在很大程度上是依赖于重金属对细胞诱导产生的氧化胁迫。

Transposition of transposable element increases its copy number and thus restructures the genome.

由于转座子的跳跃而使其拷贝数增加，从而使植物基因组的可塑性增大。

Edgewax: Waxing method recommended for lubricating release prints; treatment is with a solution of 50 grams of paraffin wax per litre of trichloromethane applied only to the edges of the emulsion side of the film.

用封腊的方法来润滑用于发行的拷贝；这种方法是用每升三氯乙烷溶解50 克石蜡形成的溶液，只用于处理胶片的乳剂面的侧缘。

Edgewax: Waxing method recommended for lubricating release prints; treatment is with a solution of 50 grams of paraffin wax per litre of trichloromethane applied only to the edges of the emulsion side of the film.

片边腊润：用封腊的方法来润滑用于发行的拷贝；这种方法是用每升三氯乙烷溶解50克石蜡形成的溶液，只用于处理胶片的乳剂面的侧缘。

Now is everyone that uses these forms an uncreative copy-cat?

现在是每个人都使用这些表格的一个uncreative拷贝猫吗？

The assembly code is much worse, with staggering levels of copy-paste coding, some completely useless instructions that do nothing at all, unaligned loads/stores to what-should-be aligned data structures, and a few functions that are simply written in unfathomably roundabout ways.

汇编的代码要糟糕的多，拷贝粘贴的痕迹很紧张，一些代码布局完全没用，什么也不做，应该对齐加载/存储的布局也没有对齐，有些功能使成为事实写的不知所云。D代码还看的过去，汇编代码大约是一群智障的猴子写的。

The copies are supplied free, sent to radio stations in a great unmonitored flood by the musicians' agents.

免费的音乐拷贝，从音乐人的经纪人手里以不受监控的洪水之势流到电台。

The copies are supplied free, sent to radio stations in a great unmonitored flood by the musicians' agents.

免费的音乐拷贝，从音乐人的经纪人手里以不受监控的巨洪之势流到电台。

U have set a lot of friends, often inserted U disk USB interface, in order to keep the convenience of copying without unplugging it.

很多拥有U盘的朋友，经常将U盘插入USB接口后，为了随时拷贝的方便而不将它拔下。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。