拷贝

In model organism fission yeast,Schizosaccharomyces pombe,100-200 rDNA gene copies are located on both left and right ends of chromosomeⅢ.The integrity of rDNA is critical for cell growth and survival.

在模式生物裂殖酵母(fission yeast，Schizosaccharomyces pombe)基因组中，100-200拷贝的rDNA基因分布在Ⅲ号染色体左右两端的末端附近。rDNA区的完整性对于细胞生长和存活是至关重要的。

Results The Southern blot result showed that 4 among 188 goats produced by pronuclear microinjection had integrated hEPO gene.

结果在原核注射获得的188头羔羊中，经Southern blot法检测有4头羊含有hEPO基因，其中3头为母羊，1头公羊于出生后20d死亡；3头转基因母羊hEPO基因的拷贝数分别为1、10、2；Western blot检测结果显示转基因羊乳中的hEPO分子质量为32kDa；MTT法检测结果表明，在泌乳10d的3只转基因羊乳汁中，每毫升乳汁中hEPO活性分别达到1.17×102IU、1.90×104IU、1.91×104IU。

The results of flow cytometry in normal, B27 single transgenic mice and B27/hβ〓m double transgenic mice were 0.63％, 7. 87％ and 35. 87％ respectively. B27 antigen was also expressed in the cell surface and cytoplasm of colon, skin, testis and eyes of the double transgenic mice by the detection of immunohistochemistry, which was evident on the cell surface. 8 double transgenic mice bearing high copy (7 males, 1 female) developed spontaneous alopecia, psoriasiform dermatosis, joint swelling and nail changes in the rear paw.

免疫组化检测可见转基因小鼠的结肠、皮肤、睾丸和眼睛组织的细胞膜和细胞浆均有B27抗原的存在，尤以细胞膜明显。21只B27/h〓转基因阳性鼠中有8只出现脱毛和皮肤的银屑病样改变，同时伴有后肢的关节肿胀和趾甲变化。8只小鼠均为高拷贝，其中雄性7只，雌性1只。

As we were waiting patiently for the new Striborg full length, Southwest Passage, we discovered ANOTHER new Striborg release, a split with a band called Claustrophobia, released on Sin Nanna of Striborg's own label Finsternis Productions, so we contacted Sin Nanna directly the reports of his reclusiveness, living in a shack with no telephone or computer, turned out to be greatly exaggerated!

正当我们耐心等待Striborg的新专辑《Southwest Passage》时，我们发现Striborg另一个新的作品，一张与一支叫&幽闭恐怖症&的乐队的拼盘，由Sin Nanna的Finsternis Productions出品。于是我们直接联系了Sin Nanna（有消息说他已隐居，住在一个没有电话与电脑的小屋里，这其实是夸张的说法），购买了一些这张作品的拷贝。

MIT's Rotch Library has numerous atlases that you may consult and even copy.

麻省理工学院Rotch图书馆有大量可以查阅甚至拷贝的地图集。

The multicopy gene saccharomycetes expressed recombinant plasmid pAOnsGH, the experiment shows, has very high expression activity to make the engineering saccharomycete product possess low production cost and high efficiency. Trial raising shows that feed with the said saccharomycetes added can raise the growth speed, disease resistance and output of the raised animals.

多拷贝基因酵母菌表达重组质粒pAOnsGH，经实验证明具有很高的表达活性，使本工程菌制品实现了生产成本低、效率高的效果，经多次水产养殖试验及养鸡试验，使用添加本酵母菌制品的饲料饲，喂养殖的动物可提高其生长速度和抗病害能力，提高单位养殖产量20％以上，增产的收入大于本制品成本投入的五倍以上。

Subtilis BD 224, it can be inferred that the host inheritable background could affect the segregational stability of plasmid.

这些起源相同的质粒在Baci-llus.subtilis ASl.1176中的分离稳定性存在差异，这种差异与质粒的大小和复制方式无关，而与质粒的拷贝数有一定的关系。

The open reading frame of Spinacia oleracea Betaine Aldehyde Dehydrogenase was retrieved from Spinacia oleracea and inserted into the Agrobacterium tumefaciens binary vector pBin438, which was driven by CaMV35S promoter, and produced the new binary vector pBSB. A. tumefaciens LBA4404 carrying this plasmid was used in genetic transformation of plants. Forty-five primary transgenic plants were detected by PCR and verified by the Southern blotting from 65 regenerated plants, of which 27 transgenic plants had only one copy of T-DNA.

摘 要：分离出菠菜甜菜碱醛脱氢酶基因构建成由CaMV35S驱动的双元植物表达载体pBSB，农杆菌菌株LBA4404携带该载体转化棉花，获得转基因棉花植株。65株转基因植株经过PCR筛选、Southern blotting分析证明有45株为成功的转化株，外源基因已经被整合到棉花的染色体组中，并以单拷贝插入居多。

By PCR amplification and southern blot analysis, the extrogenous gene with specific promoter was confirmed to integrate into the supersweet maize genome DNA with single-copy, and transformation frequency could reach as high as 9.09%.

经PCR和Southernblot分析，证明抗虫基因已经整合在超甜玉米基因组中，并且为单拷贝。T1代植株PCR检测结果表明，Bt基因能够在转基因后代中稳定遗传，基因分离符合1∶1的孟德尔遗传分离规律。

The aim is to gain transgenic lines resistant to Botrytis cinerea, and test the synergistical effects of bivalent gene.

绿色荧光蛋白基因活性检测，PCR和Southern杂交检测表明，外源基因分别以1～4个拷贝整合到番茄基因组中。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。