拷贝

The XYN7 gene, located on multicopy shuttle vectors pYES6/CT, was successfully expressed in the yeast Saccharomyces cerevisiae under the control of the GALI promoter and CYC1 transcription termination signal , respectively.

XYN7基因构建在多拷贝穿梭载体pYES6／CT上，在GALI启动子和CYC1转录终止信号的控制下成功在酿酒酵母中表达。

Have a plant area of 6000 square meters, with four-color, two-color Roland printing presses, automatic die-cutting machine, automatic machine, automatic paper mounting machine, grinder, folder gluer, such as a set of printing equipment, in the design of the production the better film, plate skilled personnel, computer and automatic punching machines and other copy-plate equipment, able to exert the maximum effect of the printing.

拥有厂房面积达六千平方米，拥有四色、双色罗兰印刷机、自动模切机、自动过胶机、自动裱纸机、磨光机、糊盒机等一整套印刷设备，在设计制作菲林上更胜一筹，技术精湛的制版人员，电脑和自动拷贝冲片机等制版设备，能最大限度的发挥印刷的效果。

Have a plant area of 6000 square meters, with four-color, two-color Roland printing presses, automatic die-cutting machine, automatic machine, automatic paper mounting machine, grinder, folder gluer, such as a set of printing equipment, in the design of the production the better film, plate skilled personnel, computer and automatic punching machines and other copy-plate equipment, can play a maximum printing results.

拥有厂房面积达六千平方米，拥有四色、双色罗兰印刷机、自动模切机、自动过胶机、自动裱纸机、磨光机、糊盒机等一整套印刷设备，在设计制作菲林上更胜一筹，技术精湛的制版人员，电脑和自动拷贝冲片机等制版设备，能最大限度的发挥印刷的效果。

Southern blotting suggested that at least two copies of GhPex7 gene existed in Gossypium hirsutum genome.

Southern杂交结果表明该基因在陆地棉基因组中存在两个拷贝。

We build more computers tohold more information, to produce more copies, but haveless communication .

我们生产出更多的电脑来存储更多的信息，制造更多的拷贝，而相互之间的交流与沟通却越来越少。

These sequences possessed highly copy number in the genome and were named as RMX1-6 individually. When these sequences were hybridized to chromosomes of spiny eel, RMX6 was proved to be sex chromosome specific and it only located in heterochromatic regions.

在随机挑取的三百多个克隆中，分离得到了6个高度重复序列，它们在基因组中存在较高拷贝数，按照发现顺序分别命名为RMX1—6(Repetitive sequence inMicrodissected X chromosome)。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

High copies and the homoplasmic mutation in tumor, mtDNA mutations may be attractive diagnostic markers for some cancers.

由于肿瘤中mtDNA的高拷贝、同质性突变等特点，有人提出mtDNA突变有希望成为肿瘤早期诊断的分子标记。

Results There are 62 patients infected by UU or CT in all of 116 infecund and the can not bear patients(the infections rate is 53.4%). The rate of infected by UU is 42.25. the rate of infected by CT is 17.2%. The rate of infected by both UU,CT is 6.0%. Man's rate of infected by UU ,CT is 35.9%,21.9%. Woman's rate of infected by UU ,CT is

结果 116例不孕不育患者中有62例感染UU或CT（感染率53.4%），UU和CT的感染率分别为42.2%和17.2%，混合感染率6.0%；男性UU和CT感染率分别为35.9%和21.9%，女性UU和CT感染率分别为50.0%和11.5%；女性患者UU和CT的基因拷贝数均高于男性。

But it is also difficult to identify a mutant related to Tos17 because there are multiple copies of Tos17 in a mutant and only about 10 percent of mutants are from Tos17 insertion. Theoretically, Ac/DS two-element system is regarded as a best approach for rice insertional mutagenesis.

Tos17是目前应用最为成功的反转录转座子，但多拷贝的插入使突变体的表型鉴定和分子鉴定较为困难，因为只有10%左右的突变性状是由Tos 17插入引起的。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。