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In order to avoid prolin residue inhibiting enterokinase cleavage, 9bp of MGF cDNA 5′ end sequence was truncated by primer, then the obtained truncated MGF des(1-3MGF cDNA (321 bp) was subcloned in pET32a vector to construct a prokaryotic recombination expression plasmid.

通过RT-PCR从拉伸刺激的人成骨细胞中克隆MGF cDNA序列,并去除5'端9 bp的序列,使N端缺少对肠激酶(Enterokinase, EK)具有抑制作用的脯氨酸,将截短型MGF des(1-3 MGF cDNA序列克隆入pET32a质粒,构建重组表达质粒。

His basic message is that only those able to turn themselves into monomaniacal workaholics estranged from loved ones and reviled by rivals - or willing to unsheathe their inner monster - can hope to hit the mega jackpot.

他书中传达的基本信息是,只有那些能把自己变为工作狂、让亲者痛仇者恨的人,或是拉得下恶脸的人才有望积累起巨额财富。

But whereas Wodehouse's wit was gentle and warm, Mr Latimer empties buckets of bile on almost everyone he has ever met or worked with.

然而,如果说沃德豪斯的机智表现得既温和又热情,拉蒂默先生却恨不得把怨气撒在每个他遇到过的或是共事过的人身上。

President Abraham Lincoln's an honoree, too, and not for his legendary woodchopping( woodchopper的变形:劈柴者,伐木者,砍柴人,樵夫) skills.

亚伯拉罕·林肯总统同样也是名人堂的一员,但这并非是因为他传奇的伐木本领,而是因为他签署法令开发西部来建设家园,设立了农业部并在农村地区广立了大学。

But with people such as Zander and yourself, Motorola symbolizes the worst of our country's corporate culture.

但是因为Zander和你这样的人的存在,摩托罗拉象征着我们国家企业文化中恶劣的一种。

But with people such as Zander and yourself, Motorola symbolizes the worst of our country\'s corporate culture.

但是有了Zander和你这样的人在公司,摩托罗拉反而成了美国最差劲的公司文化的缩影。

Such as Zander and yourself, Motorola symbolizes the worst of ourcountry's corporate culture.

但是因为Zander和你这样的人的存在,摩托罗拉象征着我们国家企业文化中恶劣的一种。

In Mrs.在夫人 Aphra Behn 's play,"The Widow Ranter, or Bacon in Virginia," the scene is laid in America, the subject dealing with the insurrection known as Bacon's.

阿芙拉贝恩氏发挥,&在弗吉尼亚州的寡妇豪言壮语的人,或培根,&现场是在美国规定,与被称为起义处理该问题的培根。

"China is becoming easy -- people who cannot speak Chinese or English can come here now," Ashraf Shahabi, 29, said between greeting customers at his Al-Arabi restaurant, one of a dozen Arab eateries lining Yiwu's Exotic Street.

&中国正变得容易-不会说中文或英文的人现在可以来这里,&阿什拉Shahabi,29日表示,他与的客户在在义乌异国风情街。阿拉比餐厅里,这是12个阿拉伯国家的餐馆之一

"Find how to fix longitude and you're the richest man in the world," his old teacher, Alban Caradoc, had said.

&要是你能想法儿修正航行中的经度差,你准能成为这世界上最有钱的人,&布莱克松记得他的老师——奥班·凯拉多克老先生曾经告诉他。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。