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The effect of supplementing ammonium sulfate, peptone and yeast extract on the rljGH production was further investigated.

进一步考察了在诱导阶段添加硫酸铵、蛋白胨和酵母抽提物的影响。

Hordenine propyl formate and chemical osmosis are the two ways of extracting Glutathione from yeast have been introduced and compared in this review.

介绍了从酵母中分离纯化谷胱甘肽的两种抽提方法:对羟基苯甲酸丙酯法和化学渗透法,并对其进行了对比。

In extracts of source rocks of shahejie 1 group of Gulan low-lying abundant retene, simonellite and cadalene were detected. Their main origin should be lower organisms like algae.

在孤南洼陷沙一段烃源岩抽提物中检测到了较为丰富的惹烯、西蒙内利烯及卡达烯,其主要来源应是藻类等低等植物。

Complex extraction technique of yeast gourmet from waste saccharomyces cervisiae was studied in the paper.

研究了从啤酒废酵母中抽提酵母味素的综合技术。

The lengths of both A and I bands increased with the sarcomere length, the A band increasing even more markedly than the I band.

用离子強度大于1.5的氯化鉀等混合液(Hasselbach和Schneider溶液)抽提,不能将A带的蛋白抽出。

The article introduced the development of the yeast extract and the application in food and seasoning briefly.

酵母抽提物是一种优良的天然调味料,在食品行业中具有广泛的用途。

The extractingmethod for seed and leaf of Ginkgo biloba and RAPD experimental techniquefor Ginkgo biloba were established.

在该项实验分析过程中还建立了适合银杏种子和叶子抽提DNA的方法及适合银杏的RAPD实验技术方法。

A pair of primers based on conservative sequences of Drosophila melanogaster was designed to amplify the p53 gene. The fragment with 1156 base pair was obtained and sequenced. The percent identity between others' and ours' was 99.4%, with the other biological sequence of p53 existing some differences.

从紫外刺激的果绳抽提总RNA,通过RT-PCR方法扩增得到有完整读码框的p53基因,并且通过对p53基因的序列分析表明,所得到的序列与网上已经登陆的果蝇p53序列有99.4%的相似系数,而与其他生物的p53序列存在一定的分歧。

Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外,培养人脑胶质瘤U251细胞,进行细胞总RNA抽提,运用RT-PCR方法进行TfR基因的完整编码区外显子的扩增,经过酶切、连接后构建pCDNA3.1-TfR的表达质粒,鉴定正确后按脂质体转染方法转染人胶质瘤U251细胞,使其过量表达,通过pEGFPN1-TfR检测其瞬时转染的效率;用G418对稳定表达pCDNA3.1-TfR的细胞进行筛选,挑选阳性细胞克隆,运用Western blot、流式细胞检测技术和细胞免疫化学以及mRNA半定量的方法检测TfR在转染前后的表达水平,鉴定转染的效果。

Mann-Whitney signed rank test was used to assess differences between continuous nonnormally distributed variables and multiple linear regression was used to model LOS and direct hospitalization costs.

用SPSS11.0对抽提数据进行统计描述,不进行数据合并。2、设计前瞻性病例观察实验,对第二军医大学长海医院烧伤科于2005.1.1-2006.12.31期间收治的儿童烫伤患者进行病例分析。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。