抽提

DNA were abstracted by means of phenol chloroform, amplification and cataphoresis test of polymerase chain reaction of target DNA,primer sequence of gene segment amplification of 5-hydroxytryptamine (5-HT) 2A receptor gene T102C: 5'-TCT GCT ACA AGT TCT GGC TT-3', 5 '-CTG CAG CTT TTT CTC TAG GG-3'; Reaction system of 50 μL, DNA 0.5 μg,Primer 50 pmol,TagDNA enzyme 2U were added with dNTP to the final concentration of 200 μmol/L.

应用常规苯酚氯仿法抽提DNA，目的DNA的聚合酶链反应扩增及电泳检测，5-羟色胺2A受体基因T102C基因片断扩增的引物序列：5'-TCT GCT ACA AGT TCT GGC TT-3'。5'-CTG CAG CTY TTT CTC TAGGG-3'；采用50 μL反应体系，DNA 0.5 μg，引物50 pmol，TagDNA酶2U加dNTP至终浓度200μmol/L。

GC-MS shows that chlorophenol and chloroguaiacol are found in the spent liquors of the first two stage (chlorination and caustic extraction). After the mixed bleaching effluents are treated by white-rot fungus P.

GC-MS分析结果表明，苇浆CEH漂白的氯化段和碱抽提段废水中均含有氯代酚和氯代愈疮木酚，而次氯酸盐处理段废水中，没有检出氯化酚类化合物。

This project also is machine from colza, arrive compress and extraction, produce what produce a process thoroughly to apply first to rough oily treatment and biology derv again.

该项目也是从菜子加工，到压榨和抽提，再到粗油加工和生物柴油生产完整的生产过程的首次应用。

The axial variance of tissue percentage of resin canals and aether extractives are coherent in Cedrus deodar, and there are positive relations between them.

雪 松试材树脂道组织比量与雪松乙醚抽提物含量轴向变异规律较为一致，呈正相关。

Methods A total RNA from ten cases of ovarian cancer and contral normal ovarian tissues were extracted by a single step method. The cDNA retro-transcribed from equal quantity mRNA derived from ten cases of ovarian carcinoma and normal ovarian tissues, which were labeled cDNA strand with Cy5 and Cy3 fluorescence as probes.

按一步法分别抽提10例人卵巢癌组织和对照正常卵巢组织的总RNA并纯化mRNA；分别从10例取等量的人卵巢癌组织和对照组织的mRNA分别混合后逆转录合成以Cy5和Cy3标记的cDNA一链做探针，混合后在含有8464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

Methods］ A total RNA from 10 cases withovarian papillary serous cystadenocarcinoma and normal ovarian tissues were extracted by a single step method of gene chip. The cDNA retro?鄄transcribed from equal quantity mRNA derived from 10 cases with ovarian papillary serous cystadenocarcinoma and normal ovarian tissues. The cDNA strand was labeled with fluorescent Cy5 and Cy3(normal ovarian epithelial cells) as probes.

方法］ 采用基因芯片技术，按一步法分别抽提10例卵巢浆液性乳头状囊腺癌患者的癌组织和正常卵巢组织的总RNA并纯化mRNA；逆转录合成以Cy5荧光标记卵巢癌细胞和Cy3荧光标记正常卵巢上皮细胞的cDNA作为探针，在含有8 464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

Methods The cDNA retro-transcribed from equal quantity total RNA derived from rectal carcinoma or normal rectal mucosa were labeled with Cy5 or Cy3 fluorescence as probes respectively. The mixed probes were hybridized with the BioStar 4096 cDNA microarray.

分别抽提直肠腺癌和正常直肠粘膜的总RNA；通过逆转录方法将Cy3和Cy5两种荧光分别标记到两组cDNA上，制成探针；利用这种cDNA探针与含有4096条人类基因PCR产物的表达谱芯片进行杂交，扫描芯片荧光信号图像，计算机分析后比较两种组织中差异表达的基因。

Is the largest professional yeast producer in Asia, as well as the leading enterprise of yeast extract and drafter of national standard, the YE production line adopts the most advanced technology and equipment in the world, and the product quality reaches the world leading level.

安琪酵母股份有限公司是亚洲规模最大的专业化酵母生产企业，也是酵母抽提物的龙头企业和国家标准的起草方，其YE生产线运用了全球最先进的技术和设备，产品质量达到世界领先水平。

When Tetrahymena, Euglena, Crypthecodinium and Oxyrrhis were stained with the same technique, although all the nuclei gave positive reactions, no precentromeres could be distinguished; within nuclei there were only patches composed of minute fluorescent spots seen.

在对小眼虫和寇氏隐甲藻作制备核骨架的细胞分级抽提处理后，细胞核仍还都呈阳性反应。这表明与高等真核生物的相似，低等真核生物的着丝粒蛋白也是与核骨架紧密结合的。

Objective: To explore a method of extracting RNA directly from whole blood and approach to storage.

目的：对全血中直接抽提RNA的方法及标本的保存方式进行探讨。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。