抽提

Pure mitochondrial DNA was successfully obtained by using SDS and proteinase K to crack mitochon, phenol: chloroform: isoamyl alcohol extraction to remove protein, and digestion with RNase A.

用SDS和蛋白酶K裂解线粒体，经酚：氯仿：异戊醇抽提除去蛋白，并用RNaseA消化从而得到单纯线粒体DNA。

Methods Retinal pigment epithelium cell line ARPE-19 were put into hypoxic chamber for 24 hours and RNA were extracted for detection further.

方法视网膜色素上皮细胞株ARPE-19置缺氧环境24小时后，抽提RNA进行MicroRNA芯片(LC science公司)检测。

The expression of MMP-8 was examined by immunohistochemical technique in 50 cases of pterygia and 10 cases of normal conjunctival tissues.

翼状胬肉标本10例抽提总DNA并行RT-PCR扩增，探讨MMPs基因表达在翼状胬肉中的作用。

An improved method that facilitates the extraction of PCR-compatible faecal DNA from giant panda\'s faeces is described. The method involves a novel preprocessing step in DNA extraction. The faeces is washed two or three times with precooled acetone, which removes numerous potential PCR inhibitors, and then digested with proteinase K. The DNA is purified with phenol/chloroform.

在粪便DNA的提取过程中采用一个新的预处理方法，将粪便用预冷的丙酮洗2～3次，除去粪便中含有的大量PCR抑制物，然后蛋白酶K裂解、酚-氯仿抽提，能提取到纯度很高的DNA供PCR扩增。

Beef powder is produced from high quality Dabie mountain scalper and beef bone extraction through steps of ''biological enzymolysis, mailard reaction with reducing sugar and kinds of amino acid, low temperature spray dried after taste change and flavor adjust''. It mostly keeps beef's fragrance,flavor and nutrients.

牛肉精粉是以优质的大别山黄牛肉和牛骨抽提物为原料，经生物酶解后与还原糖和各种氨基酸发生美拉德反应生香，再经口感调理和适度调香后，采用国内最先进的低温真空干燥设备，干燥而成，极大的保留了牛肉的香气，香味和营养物质。

Beef powder is produced from high quality Dabie mountain scalper and ox bone extraction,the material make fragrance by mailard reaction with reducing sugar and kinds of amino acid after biological enzymolysis, and through low temperature spray drying after taste improving and flavor change. It mostly keep the fragrance,flavor,nutrients of beef.

牛肉精粉是以优质的大别山黄牛肉和牛骨抽提物为原料，经生物酶解后与还原糖和各种氨基酸发生美拉德反应生香，再经口感调理和适度调香后，采用国内最先进的低温真空干燥设备，干燥而成，极大的保留了牛肉的香气，香味和营养物质。

Under the condition of consumption of raw material naphtha not to be increased, two processes of pre-hydrogenation and pre-extraction was developed in the ethylene complex project and the investment and operation cost evaluated.

在不增加石脑油原料消耗量前提下，开发乙烯联合装置裂解原料前加氢和前抽提2个改进流程，并计算该装置的工程投资与运行费用。

Facing this condition, study of manufacturing yeast extract and nutritions soybean sauce is carried out, and satisfactory effect is obtained.

针对这种情况，我们利用本厂啤酒废酵母进行了生产酵母抽提物及营养酱油的研究，取得了满意的效果。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

Three tar mat s of Well Wenjian3 were discovered and determined accurately in Wenliu Oilfield in Dongpu Depression by thin layer chromatogram-flame ionization detector, whole hydrocarbon gas-chromatography, and the characteristics of reservoir sedimentology and logging response.

根据文留油田文检3井密闭取心层段岩心样品抽提物薄层色谱分析和全烃气相色谱分析、以及储层沉积学和测井响应特征发现并准确鉴定出3个沥青垫。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。