抽提

MethodsJirihen Agaru-8 polysaccharide was extracted with water. The herb was deproteined, ethanol precipitated, and purified with dialyses method.

方法吉日很阿嘎如-8经热水抽提，Sevag法脱蛋白，乙醇沉淀，再通过透析得白色粉末状精制多糖。

Since the post-translational modification process doesn't exist in E. Coli expression system, in order to investigate if the natural nsLTP has antimicrobial activity, we purified nsLTP from rice etiolated seedlings. They were shown to have the similar antifungal activity compared with E. Coli expressed nsLTP.

由于大肠杆菌表达产物缺少翻译后加工，为了与表达产物的功能研究相对照，还摸索了从水稻黄化苗中抽提nsLTP的方法，结果表明水稻苗中提取的nsLTP亦具有抑菌活性，抗性水平接近大肠杆菌表达产物。

First, the anatomical features, physical features and extraction contents of small-diameter lumbers were studied systematically and compared with mature wood. The reason that drying defects occurred easily in small-diameter lumber was discussed based on its properties.

首先，测定了小径木的解剖学特性、物理特性和抽提物的含量，并与成熟材进行了比较，根据小径木木材的上述性质探讨了小径木锯材产生干燥缺陷的原因。

Methods and materials A549 cells were collected and lysed for total protein after treated with metformin (5 mM) for 0, 15, 30 and 60 minutes. Western blot analysis was performed to detect the expressions of pan- and pho- AMPK, JNK and p38 proteins. Cleaved Caspase-3, 8, 9 proteins were also detected.

以5mmol/L的二甲双胍对A549细胞进行体外干预，在干预的第0、15、30和60min时，分别抽提细胞总蛋白，以Western blot技术检测细胞内AMPK、JNK、p38 MAPK的总蛋白和磷酸化蛋白的表达；在干预后第0、12、24和48h，检测剪切后的Caspase-3、8、9活性蛋白的表达水平。

First, nucleated cells were separated from fresh peripheral blood and the total RNA was isolated from nucleated cells.

首先分离外周血有核细胞，进行细胞总RNA的快速抽提。

Methods The drying soybean products were triturated. The powder samples were defated by the diethyl ether for 5 hours, and returned by the 80% methyl alcohol solutions with the liquid-solid ratio of 25:1 for 5 hours. The content of the total isoflavone in filtrate was determined by ultraviolet spectrophotometry.

将烘干的豆制品研成粉末，取粉末样品置于索氏抽提器中，用乙醚脱脂5h后，用80%的甲醇溶液以25：1的液固比热回流5h，用紫外分光光度法测定滤液总异黄酮含量。

In order to establish the simple extraction method of wheat mitochondrial DNA, this study investigated the effects of triturating material, exacting mitochondria and exacting and purifying mtDNA from yellow seedling of wheat, respectively.

为了建立小麦线粒体DNA快速提取体系，以小麦黄化苗为材料，分别研究了材料的研磨、线粗体的抽提和mtDNA的提取及纯化过程中众因素的影响。

In order to improve the content of phosphatidylcholine, a study has been carried out on the use of acetone and alcohol to extract PC from soybean lecithin .

研究了以浓缩大豆磷脂为原料，采用丙酮和乙醇作溶剂，经丙酮脱油、真空干燥、乙醇抽提、减压蒸馏浓缩制备高含量磷脂酰胆碱的提纯工艺。

The cytoplasmic extract was obtained through the lysis buffer containing high concentration of salt, eradicator and glycerol.

用含有高盐、高去污剂、高甘油的细胞裂解缓冲液制得了细胞抽提物。

This paper mainly studies the sizing techniques and mechanism of self-fixed neutral sizing agent WSF- I when used in the bleached baggase pulp0 The combination of crossing design and uniformity design scanning electron microscope observation and organic dissolvent Extraction were used to optimize and analyze in this research. Including three aspects:(1)to search for the optimal sizing techniques;(2)to analyze the factors affecting the sizing;(3)simply studies the sizing mechanism.

本文主要采用正交设计和均匀设计的试验方法、并结合电子显微镜观察和有机溶剂抽提等分析手段对自定型中性施胶剂WSF-I在漂白蔗渣浆施胶中的施胶工艺和施胶机理进行了研究，研究内容主要有三个方面：①最佳施胶工艺的探索；②影响纸页施胶度的因素的分析；③施胶机理的初步研究。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。