抽提

Methods Extract α 1 AT from FIV 1,precipitate with PEG,purify by ion exchange chromatography and lyophilize.

FIV 1抽提液经PEG沉淀，离子交换，病毒灭活、超滤、除菌、分装，冻干制备α1 AT制剂。

Have unit of 6 main production; Produce per year device of device of 130 Kt butadiene styrene rubber, 38 Kt butadiene rubber, 56 Kt and device of extraction of 53 Kt butadiene each one, device of aether of butyl of 40 Kt methylic father's younger brother, 15 Kt 1 - butene device.

现有 6套主要生产装置；年产 130 kt丁苯橡胶装置、 38 kt顺丁橡胶装置、 56 kt和 53 kt丁二烯抽提装置各一套、 40 kt甲基叔丁基醚装置、 15 kt 1－丁烯装置。

Therefore, the aim of this research is to compare the antioxidant, acetylcholinesterase-inhibiting and monoamine oxidase -inhibiting activities of these four parts of Morus alba. In addition, I also explored the mechanism of the ameliorating effects of scopoletin on the scopolamine induced memory impairment in rats.

因此，本研究针对桑四个药用部位之甲醇抽提物进行抗氧化活性及对乙醯胆碱酯酶、单胺氧化酶的抑制活性之分析比较，之后再探讨其香豆素类成分 scopoletin对 scopolamine 诱发大鼠记忆障碍之影响，及其改善作用机制。

Methods Blood samples were obtained from 5 patients with AIDS, the C2~C3 region of HIV-1 envelope gene was amplified by nested polymerse chain reaction from plasma of each patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous and nonsynonymous substitutions as well as ds/dn ratios were calculated.

采用套式聚合酶链反应方法对5例艾滋病患者血浆HIV-1 env基因C2~V3区进行PCR扩增，产物经纯化后克隆至pMD-18T载体中，每例患者分别挑取5~8个克隆，共34株，完成阳性克隆的鉴定、抽提纯化和测序，将获得的DNA序列及核苷酸序列进行分析，并计算其核苷酸同源替换率与非同源替换率的比值。

Blood samples were obtained from 5 patients with AIDS, the C2~C3 region of HIV-1 envelope gene was amplified by nested polymerse chain reaction from plasma of each patient, and then subject to sequencing, clonal-quasispecies analysis, and the accumulation of synonymous and nonsynonymous substitutions as well as ds/dn ratios were calculated.

采用巢式PCR方法对5例艾滋病患者血浆HIV-1env基因C2~V3区进行PCR扩增，产物经纯化后克隆至pMD-18T载体中，每例患者分别挑取5~8个克隆，共34株，完成阳性克隆的鉴定、抽提纯化和测序，将获得的DNA序列及氨基酸序列进行分析，并计算其氨基酸同源替换率与非同源替换率的比值。

Methods: Matched 24 pairs normal and tumor samples were collected from who underwent surgery for oesophageal squamous cell carcinoma.

收集24对原发性食管癌的癌组织和相应正常食管组织，分别抽提基因组DNA。

Our study show that 9, 11-Octadecadienoic acid, methyl ester is the highest content of organic acid ester components in non-hydrocarbon fraction, higher content of 6-Octadecenoic acid, methyl ester reflects mainly terristrial source input, higher content of pentadecane acid, 14-methyl, methyl ester or Heptadeeanoic acid, 16-methyl, methyl ester shows mainly aquatic source input.

原油和岩石抽提物非烃中有机酸酯类化合物的有机地球化学研究报道很少，系统研究发现非烃有机酸酯中9，11-十八二烯酸甲酯含量最高，而次高峰的丰度可用作生源判识，陆源母质输入多的样品次高峰为6-十八单烯酸甲酯，水生母质供给多者次高峰为14-甲基十五烷酸甲酯或16-甲基十七烷酸甲酯。

There is a great difference in the gross composition and biomarker distribution between chloroform extracts of coal and oil from coal measures, which results in a difficulty in oil-source correlation in a coal-bearing petrolific basin.

煤的氯仿抽提物族组成和生物标志物分布特征与煤系原油存在很大差别，导致含煤沉积盆地中煤系原油的油源确认十分困难。

The Charon library DNA was then digested with restriction enzymes BamH Ⅰ and Hind Ⅲ, and DNA fragments of length of about 0.5-1. 5kb was subcloned into plasmid pUC 18 which was also digested by BamH Ⅰ and Hind Ⅲ and dephosphorylated by alkaline phosphatese before being ligated with Charon fragments.

抽提λCharon文库总DNA，用BamHⅠ和HindⅢ完全酶切电泳，用低熔点琼脂糖回收长约0.5-1.5kb的DNA片段；连接到pUC18上，pUC DNA先用BamHⅠ和HindⅢ酶切，后用碱性磷酸酯酶脱磷。

A rapid and effective' test of SOD activity in fresh tomato by the polarographic oxygen electrode is reported in this paper.

本文用极谱氧电极法检测新鲜西红柿中SOD的活力，使用了不同的抽提方法，结果表明用pH7。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。