抽提

Immobilized metal-chelated affinity chromatography column sorbents matched with Zn2+ were prepared for purifying glutathione using chitosan spherule as substrate.

以谷胱甘肽的酵母抽提液为原料，用自制的以壳聚糖小球为载体、Zn2+为配基的金属螯合亲和层析柱分离纯化谷胱甘肽。

Some approved aneugens Trip terygiuwlIypogaucum (evelHutch, colchicine, thiabendazole, a tropine sulfate,d-ubocurarine was added into the reactive system respectively and the changes of their absorption values were taken down during different periods under the conditions of 37 0C and 350nm .

本研究通过多步聚合和解聚合反应从猪脑中分离纯化微管蛋白，建立微管蛋白的体外聚合和解聚合反应平衡体系，并将已证实的非整倍体诱发剂（昆明山海棠根部水抽提物、2-（4′-噻唑）苯丙咪唑、硫酸阿托品、d-筒箭毒碱）分别加入此反应平衡体系中，于37℃、350nm条件下，记录此体系不同时期的吸光值变化，与阳性对照物和溶剂对照处理平衡体系后的吸光值在相同时间点作比较，以此评估这几类非整倍体诱发剂对离体条件下的微管蛋白聚合的影响，寻求这几类非整倍体诱发剂是否可通过抑制微管蛋白聚合作用途径而诱发非整倍体。

Results Hypericin(75w tungsten lamp,10 min of light exposure,2μg/ml)had obvious cytotokic ef- feet on C6 cells, and the specific DNA...

结果金丝桃素在低剂量2μg／ml时，75W钨灯光照10min，即可对C6细胞株具有细胞毒作用；抽提其DNA做琼脂糖凝胶电泳可显示凋亡特有的DNA"阶梯"。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

Roots were collected from the seedlings after inoculating pathogen Verticillium dahliae with 2, 4, 8, 12, 24, 48, 72 and 96 hours and used for total RNA extraction.

在Pima 90幼苗接种黄萎病病原菌后2、4、8、12、24、48、72和96 h分批取幼根以抽提棉花总RNA。

The effect on the wettability of desity and extractives in five kinds of wood species was also studied.

分析了5个树种的木材的密度、抽提物对表面润湿性的影响。

The treatment of Masson pine with white rot fungus was studied and the degradation effects of lignin and dichloromethane extractives in short period were reported in this work.

使用白腐菌处理马尾松木片，在短期内对木素和DCM抽提物的降解情况进行了研究。

In China, more and more food enterprises begin to accept the yeast extract.

在中国，酵母抽提物目前也逐渐被越来越多的食品企业所接受。

Organic acids such as stearic acid, abietic acid,α-naphthylacetic acid, can interact with copper.

在索氏抽提的条件下，铜能和有机酸反应生成金属铜的有机酸配合物。

With Actinidia which is abundant in secondary metabolites of polysaccharide and polyphenol as materials, the extraction effect of different variety and method on the genomic DNA of Actinidia was compared with, and influence of the different part of tissue culture, antioxidant and the method of DNA purification on the yield of genomic DNA was studied.

方法］以富含多糖和多酚次生代谢产物的猕猴桃为材料，比较不同品种在不同方法下的基因组DNA抽提效果，并对不同材料部位、抗氧化剂以及DNA纯化方法等对基因组DNA的获得进行研究。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。