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METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

无菌抽取的骨髓经肝素化后,采用密度梯度离心法及贴壁筛选法分离获得人骨髓间充质干细胞,传至第5代按1× 108 L-1接种于6孔板内,设立2组,依达拉奉组细胞达50%融合时用含碱性成纤维生长因子、胎牛血清的L-DMEM预诱导24 h,PBS洗涤后再用20 mg/L依达拉奉无血清L-DMEM诱导24 h;空白对照组始终用含体积分数为10%胎牛血清的L-DMEM培养,不加任何预诱导剂和诱导剂。

objective to investigate the weight control behav iors and the related factors to middle school students, and to provide bases forpreventing health-risk behaviors.methodsquestionnaire surv ey was conducted among a cluster strati fied sample of 4 542 middle school students of grade one in junior middle schooland grade two in senior middle school.

目的 了解广州市中学生减肥行为现状及其影响因素,为预防中学生健康危险行为的发生提供依据。方法按随机分层整群抽样方法,抽取广州市4所中学初一到高二5个年级共4 542名中学生进行问卷调查;通过家长问卷收集学生的家庭背景资料,分析减肥行为与家庭因素的关系。

Aspiration of the subdeltoid bursa and AC joint yielded some fluid and a small piece of white material, which demonstrated monosodium urate by polarized microscopic study.

细针抽取三角肌下滑液囊和肩峰锁骨关节得到分别得到一些液体和一小点白色物质。在极光显微镜下显现monosodium urate。

The other is to use a linear neural network for feature extraction, which is simultaneously trained with the subneural networks dealing with different situations.

另一种方法是应用线性神经网络进行特征抽取,并与各子神经网络进行统一训练。

Objective To probe into the relationship between the baldder irrigation speed and unstable bladder after suprapubic prostatectomy viablader.

目的 探讨耻骨上经膀胱前列腺切除术后膀胱冲洗速度与不稳定膀胱的关系。方法抽取 90例耻骨上经膀胱前腺切除术后病人,随机分为A ,B ,C三组。

Gu Ge is based on his draw-out to different multimedia content the technology that handles, will let machine study music, make it can discern the air that gives likeness, rhythm is symphonious color.

谷歌基于其对不同多媒体内容抽取并处理的技术,来让机器学习音乐,使得它能辨别出相似的旋律、节奏和音色。

Its acquisition is based on the construction of lexieal databases, the utilization of existing thesauri, classification schedules and other knowledge classification systems, the identification of related concepts and the extraction of their attributes to establish relationship among concepts.

其获取,是在构建语料库的基础上,利用叙词表、分类表和其他知识分类体系等现有的知识本体,识别领域内外相关的概念并抽取相应属性,建立概念之间的关系,并利用所识别的概念和关系创建新的本体,融合已有的本体和新建本体。

Fluid can also be collected during thoracoscopy or laparoscopy.

在胸腔镜和腹腔镜检查时可抽取液体。

Lastly, specimens contain HPV16 DNA were digested with restricted enyzme BamHI, and done with southern transprint to hybridize with digoxigenin-labeled probe of HPV16 genome through nuclear acid hybridization. Results (1)Among 60 cervial carcinoma specimens,the HPV16 E5 positive rate is 33%(20/60) by PCR, 70%(42/60)、58%(35/60)by nuclear acid dot hybridization with HPV16 E5 probe and HPV16 probe .

结果(1)PCR法 60例宫颈癌组织中HPV16 E5 片段的阳性率为33%(20/60);(2)斑点杂交法60例宫颈癌组织中HPV16 E5阳性率为70%(42/60),HPV16阳性率为58%(35/60);(3)宫颈癌组织中HPV16 E5基因的突变率为40%(8/20),迁移位置不甚一致,随机抽取一例E5变异的PCR阳性产物进行测序分析,结果发现在E5片段中有2处变异, nt3979A→C颠换,nt3988T→C转换,导致E5编码区出现1个氨基酸的有义变异。

Methods:Obtain the stromal cells of bone marrow from the trochanteric region of femur of rabbit and culture the cells with special cultural fluid in vitro.

抽取日本大耳兔骨髓液经密度梯度离心得骨髓单个细胞,以1×106/ml的细胞浓度培养,二周后得到贴壁生长的单层细胞。

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She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了,但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场,你等着挨绳子吧!

Several theories about reigniting and extinguishing of the arc have been refered.

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