报道的

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The results of physical and chemic characteristic show that the molecular weight of R-PE is 194kD, which is differ from the other reported R-phycoerythrin's (240kD). The molecular weight of subunits were determined . The molecular weight of a and P subunits are alike for about 20kD. The molecular weight of y subunit is about 31 kD.The subunit composition of R-PE in Corallina officinalis is presumably 4 y , which is differ from 6 y that is reported by other researcher. Moreover, we must to do more other work to prove if it contains the fourth subunit.

对R-PE部分理化特性的研究结果表明，R-PE的分子量为194kD，与目前报道的多数R-藻红蛋白240kD的分子量不同；其α、β亚基分子量均为20kD左右，γ亚基分子量约为31kD，理论推测的R-PE亚基组成为_4γ，与前人报道的_6γ不同，而且其是否存在第四亚基还有待进一步证明；同时在变性条件下对R-PE的各亚基进行了分离纯化并对其各亚基可见光光谱特性作了研究。

The results of physical and chemic characteristic show that the molecular weight of R-PE is 194kD, which is differ from the other reported R-phycoerythrins (240kD). The molecular weight of subunits were determined . The molecular weight of a and P subunits are alike for about 20kD. The molecular weight of y subunit is about 31 kD.The subunit composition of R-PE in Corallina officinalis is presumably 4 y , which is differ from 6 y that is reported by other researcher. Moreover, we must to do more other work to prove if it contains the fourth subunit.

对R-PE部分理化特性的研究结果表明，R-PE的分子量为194kD，与目前报道的多数R-藻红蛋白240kD的分子量不同；其α、β亚基分子量均为20kD左右，γ亚基分子量约为31kD，理论推测的R-PE亚基组成为_4γ，与前人报道的_6γ不同，而且其是否存在第四亚基还有待进一步证明；同时在变性条件下对R-PE的各亚基进行了分离纯化并对其各亚基可见光光谱特性作了研究。

The contribution of this dissertation is to identify and explain 8 different contrast factors in the motion events: 1. The factive or the fictive motion in events; 2. The motion caused by the internal or external forces; 3. The process windowing or objective windowing; 4. The live reporting or non-live reporting; 5. The Directive role or the Objective role in motion event; 6. The high or low volition of the Subject of motion; 7. The event or state; 8. The Container schema or Path schema.

本文的贡献在于揭示了位移事件中的八种对立现象：（1）现实位移和虚拟位移的对立；（2）内动力位移和外动力位移的对立；（3）过程取景和目标取景的对立；（4）现场报道与事后报道的对立；（5）位移方向与位移目标的对立；（6）高意志性和低意志性的对立；（7）事件与状态的对立；（8）容器图式和路径图式的对立。

The nucleotide sequences of the PAP gene and PAPⅡ gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPⅡ gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99. 8% were observed respectively. They both had one open reading frame . Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陆根为材料，提取其总RNA、反转录，用一对PAP基因的特异性引物和一对PAPⅡ的特异性引物，分别对反转录产物进行PCR扩增，将扩增的目的片段进行序列分析，结果扩增出来的PAP基因与已经报道的序列完全相同，扩增出来的PAPⅡ基因与已经报道的序列同源性也在99.8％，而且它们都各自编码一个开放阅读框架，可以说美洲商陆根中肯定存在PAP和PAPⅡ基因，并已经转录。

The nucleotide sequences of the PAP gene and PAPII gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPII gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99.8% were observed respectively. They both had one open reading frame. Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陆根为材料，提取其总RNA、反转录，用一对PAP基因的特异性引物和一对PAPⅡ的特异性引物，分别对反转录产物进行PCR扩增，将扩增的目的片段进行序列分析，结果扩增出来的PAP基因与已经报道的序列完全相同，扩增出来的PAPⅡ基因与已经报道的序列同源性也在99.8％，而且它们都各自编码一个开放阅读框架，可以说美洲商陆根中肯定存在PAP和PAPⅡ基因，并已经转录。

However a great deal remains to be done in terms of theorising what this whole experience of 'embedding' in war has to tell us about the increasingly blurred lines between journalism and public relations, about media/military relations, about the changes to television news coverage and to the very definition of 'news' being brought about by new technologies, and about differences between journalism practice and 'what the audience wants'.

但是从理论上讲还有大量遗留问题。嵌入式战争报道的经验有待回答以下问题：关于新闻和公共关系之间日益模糊的界线，关于媒介/军事关系，关于电视新闻报道的变化，新技术所带来的对&新闻&的重新定义，以及新闻实践和&观众所想要的&之间的差异问题。

Doped AgBr emulsion had an optimal amount with AgBr to silver stearate of 1∶4 in PTG materials.

由Zou以及Sahyun[1]等人在早期报道的，粘合剂采用溶剂型聚乙烯醇缩丁醛，卤化银采用原位法合成时的PTG材料的化学增感无效，照相速度的提高总伴随着灰雾的增加；他们认为当卤化银颗粒与羧酸银有界面存在时，传统化学增感的效果不明显；由Yamane[2]等人于近期报道的

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐