报告

Methods: In accordance with conventional reporter gene analysis reagent preparation method, the main component Luciferase, Luciferin and ATP were serial diluted to find the optimal concentration; Reaction solution was prepared according to the optimal concentration of the main component, then ATP were serial diluted to measure their reporter gene. Then standard curve of ATP were drawed. Tumor cell lines H1299 were serial diluted and their ATP were measured by reaction solution prepared to analyse the minimum cell number.

按照常规报告基因分析试剂配制方法，将其中主要成分Luciferase和Luciferin及ATP浓度进行倍比稀释后找到最适合反应的最佳浓度值；按照最佳浓度配制反应液，将ATP浓度倍比稀释测定报告基因数值并绘制标准曲线，分析检测灵敏度；另外选取肿瘤细胞株H1299，倍比稀释后裂解细胞应用配制好的反应液进行ATP检测，分析最低检测细胞数。

Methods:The expressive plasmids of AML1b and AML1-ETO were transfected into CV-1 and 293 cells.The expression level of endogenous pig7 gene was detected by Realtime-PCR.The luciferase reporter plasmids containing pig7 enhancer/corresponding mutant sequences were constructed and co-transfected into CV-1 cells with expressive plasmids of AML1b and AML1-ETO.The transactivity of pig7 enhancer was assayed by luminometer.

研究方法：AML1b和AML7-ETO表达质粒分别转染及共转染CV-1和293细胞，用Realtime-PCR方法检测上述基因对两种细胞内源性pig7基因mRNA表达水平的影响；构建含AML1b结合位点的pig7基因增强子序列以及相对应的突变位点的荧光素酶报告基因质粒；将表达载体/突变载体与AML1b或AML1-ETO基因瞬时共转染CV-1细胞，分析AML1b、AML1-ETO对报告基因的转录调节作用。

Fisher said the two ports will receive the Antipodes report today and Lyttelton will appoint an adviser to help review the findings in January before a formal review in February.

另外，据利特尔顿主席透露，两家公司将于今天收到来自安蒂波德斯咨询公司的报告。利特尔顿会在明年1月份对该报告进行预审，然后在2月份进行正式审核。

Computershare Hong Kong Investor Services Limited, using the mailing label at the bottom of the Change Request Form a stamp is not

本公司2009年中期报告备有中、英文版本。如阁下欲收取另一语言版本的2009 年中期报告，我们会在收

Thanks to the Program Committee headed by Professor Y. Manin and the 19 international panels, the selected 20 plenary lectures and 174 invited lectures will, I believe, represent the latest advancement and frontier achievements in our science.

由于马宁领导的程序委员会以及19个国际专家组的努力，我相信选出的20个大会报告和174个特邀报告，代表了近期数学科学领域中的前沿成果与重大发展。

More than two years passed before Vandiver Brown, head of Manville's legal department, acknowledged receipt of the report that was submitted for publication in the United States Public Health Service Report.

二年多以后，M公司法律部门的主管，V-B先生承认收到了这份被刊登在《美国公共健康服务报告》中的报告。

Smith, chief physician for Manville, issued an internal report showing that only four of 708 workers he studied were free of asbestosis.

Smith 博士在公司内部发表了一篇报告，报告表明，他所调查的708名石棉工人中仅有4人没有患石棉沉滞症。

Our projections were based on the successful launch of Manx products through Gordon Brown outlets.

本课主题 Management Themes 阅读下面关于一项出了问题的投资报告读后请讨论后面的问题机密报告关于收购戈登布朗有限公司事宜背景材料两年前戈登布朗有限公司被以 250 万英镑的价格收购戈登布朗有限公司是英格兰东北部一家专营办公家具的家庭公司曼克斯办公设备公司认为收购戈登布朗公司是在该地区扩大顾客数量的一种有效途径结果两年后戈登布朗的经营仍未盈利去年亏损 25 万英镑尽管进行了大量改进并彻底转变了管理方式该公司所拥有的顾客数量和业务量还是逐步下降分析我们已找出下列原因 1 戈登布朗在本地区是一个家庭公司的形象改进不仅改变了公司的上层管理也改变了公司的特点顾客对改进后的公司缺乏认识 2 戈登布朗的产品主要是传统的办公家具面是曼克斯办公设备却属于现代风格系列没有制定明晰的产品战略来促销这两种风格迥异的产品结论更多请进大家学习网http：//club.topsage.com 1 2 若我们的目标旨在扩大英格兰东北部的顾客群我们早该考虑到戈登布朗公司顾客们的需求若我们想改变公司面貌使其开始盈利我们必须在今后更加关注消费者需求 1 1 讨论问题若你当初处在曼克斯公司的位置你怎样对待这次收购 2。

AD MASLIN: Agent Mulder... Agent Mulder, I'm reading here a very pie-in-the-sky report about global domination plans by vicious, long-clawed spacelings? Is there going to be data to back this vague, omnibus account?

马斯林副局长：穆德探员…穆德探员，我看了这份非常异想天开的报告，有关地球被邪恶的外星人统治的计画，你有证据来支持你这个广泛、综合性的报告吗

Findings: Infants under one year of age are at greatest risk of contracting meningococcus. Children aged 1-5 years are the next highest risk group. Teens 15-19 are more susceptible to this disease as well. In 1998, Australia reported 421 cases of the disease, Canada had just 126 cases, and Japan made only six notifications.

小于一岁的婴儿有很大感染流脑的风险。1到5岁大的儿童是第二个高风险人群。15到19岁的孩子对这种疾病更加敏感。1998年，澳大利亚报告了421例这种疾病，加拿大只有126例，日本只报告了六例。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。