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抗过氧化氢酶

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Earthworms might be a good bioindicator for the contamination of roxarsone to soil. No significant influence of roxarsone contamination upon the biomass and the reproductive ability of earthworms was found, which indicated that earthworms could tolerate heavy contamination of roxarsone. 2. Effects on the important enzymatic system of earthworms The activities of PROD, BROD and MROD in P450 family were inhibited.

对蚯蚓体内重要酶系统的影响洛克沙胂对P450酶系中的PROD(7-戊氧基异吩噁唑酮-O-脱乙基酶)、BROD(7-苄氧基异吩噁唑酮-O-脱乙基酶)和MROD(7-甲氧基异吩噁唑酮-O-脱乙基酶)的活性都有抑制作用,对MROD的抑制作用最明显;在抗氧化酶系统中,对过氧化氢酶在整个试验进程中都有激活作用,而对其他抗氧化酶有时起激活作用,有时起抑制作用,规律性不明显。

Effects of water stress on the level of reactive oxygen speciesand activities of antioxidant enzymes in the leaves of wheat(Triticum durum Desf.) seedlings were investigated.Water stress resulted in significant increase in the contents of O-.2,MDA and the activities of superoxide dismutase and ascorbate peroxidase in wheat leaves.However,the activities of glutathione reductase,catalase and the content of H2O2 were not changed obviously under water stress.

分析了水分胁迫对小麦幼苗叶片中活性氧水平和抗氧化酶活性的影响,结果发现,水分胁迫能够显著提高超氧阴离子(O-2·)和丙二醛的含量及超氧化物歧化酶和抗坏血酸过氧化物酶的活性,而过氧化氢(H2O2)的含量及过氧化氢酶和谷胱甘肽还原酶的活性变化不明显。

The growth characters, the activities of antioxidant enzymes, including superoxide dismutase, catalase and guaiacol peroxidase, and peroxidase isozyme of the were measured.

测定了不同辐照剂量下新生根状茎的生长,研究了辐照原初根状茎愈创木酚过氧化物酶、过氧化氢酶和超氧化物歧化酶等抗氧化酶活性以及过氧化物酶同工酶谱的影响。

The results indicate seawater concentration has no effect on absorbency, pH, relative density, dry matter and water contents in Aloe vera leaves. While, seawater stress causes increased MDA and membrane penetration, decreased phytochroms, prolines and NO_3~--N. It is beneficial to plant growth when irrigation was done with 10% seawater, because of higher ratio of K~+/Na~+ and lower ratio of Na~+/Ca~(2+). In addition, the polysaccharide contents in Aloe vera leaves increase twice and triplication under 25% and 50% seawater irrigation respectively, the barbaloin contents increase over triplication in var. 75% seawater and over twice in var. 50% seawater and var. 100% seawater. However, when seawater concentration exceeds 25% the growth of plant was inhibited, biomass decreased, flowering time was deferred, Ca~(2+) content decreased caused by Na~+ accumulation in leaves.

结果表明:在海水胁迫下,库拉索芦荟全叶汁的吸光度、酸度、相对密度、总固形物和水分含量总体上受海水浓度的影响不大;但芦荟叶的丙二醛含量和质膜透性增加,叶绿体色素、脯氨酸和硝态氮含量减少;在10%海水浇灌下,芦荟叶细胞具有较高的K~+/Na~+比和较低的Na~+/Ca~(2+)比,对库拉索芦荟的生长有一定的促进作用;在25%海水和50%海水浇灌下,芦荟多糖含量提高了2-3倍;在75%海水浇灌下芦荟甙含量增加了3倍多,在50%海水和100%海水灌溉下,其含量也增加了2倍多;当海水浇灌浓度超过25%后,库拉索芦荟的生长明显受到抑制,生物量降低,抽苔期推迟,同时,海水浓度的增加也导致芦荟叶中累积大量Na~+,从而使得芦荟叶中Ca~(2+)含量降低,但Mg~(2+)并没有受到海水浓度的影响;用浓度低于75%的海水浇灌库拉索芦荟可以降低剖面深度在0-100cm之间的土壤盐度;其抗氧化保护酶中超氧化歧化酶的酶活力相对保持稳定,过氧化物酶的酶活力在10%海水浇灌下明显提高,随后则下降,而过氧化氢酶的酶活力则随海水浓度的升高而下降。

On the other hand, so far as we know, the characterized catalase is the first dimeric monofunctional catalase from alkaliphiles and is also the first monofunctional catalase derived from a natural soda lake, which could partially reflect the oxidative stress response in the corresponding environment.

另一方面,本文所研究的过氧化氢酶是第一个来源于嗜碱微生物的同源二聚体单功能过氧化氢酶,也是第一个来源于天然碱湖的单功能过氧化氢酶,它能部分地反映出细胞抗氧化体系对相应环境的适应情况。

The six stages involved euthermic state (25℃), prehibernation (10-12℃), after hibernating for a week and a month (2-4℃), and arousing from hibernation for a week and two weeks (10-12℃). From euthermic state to long-term hibernation, lipid peroxidation was showed to increase in brain except the stage of prehibernation.

分别测试了上述各阶段荒漠沙蜥1%脑组织匀浆、0.5%心脏组织匀浆及1%肝脏组织匀浆中:丙二醛及过氧化氢(H_2O_2)的含量,超氧化物岐化酶、过氧化氢酶、谷胱甘肽过氧化物酶的活性,及总抗氧化能力的水平。

The physiological and biochemical resistant mechanism of tea cultivars to tea brown blight caused by Guignardia camelliae. Making use of the mensuration of spectrophotometric analysis to study the enzyme activity of Peroxidas, Polyphenol Oxidas, Catalas, Phenylalamine Ammenia-lyase. The result showed that there was definite difference to the metabolic rule of defence enzymes activity in the annual leaf tissue of the resistant and susceptible cultivars after infecting.

茶树抗云纹叶枯病的生理生化机理研究利用分光光度计对茶树叶片中过氧化物酶、多酚氧化酶、过氧化氢酶,苯丙氨酸解氨酶的活性进行了测定分析,结果表明:感染茶云纹叶枯病菌前后,抗病品种和感病品种当年生成叶中防御酶活性变化规律存在一定的差异。

The activities of superoxide dismutase, catalase, peroxidase and malonyldialdehyde were determined in hepatopancreas in oriental weatherfish Misgurnus anguillicaudatus exposed to different concentrations 0.025mg Cd(Ⅱ/L, 0.25 mg Cd/L and 2.5 mg Cd/L of Cadmium chloride Cd(Ⅱ to evaluate the effects of cadmium stress on xanthine oxidase and antionxidant enzyme activities.

研究了不同浓度的cd对泥鳅肝脏内抗氧化酶包括超氧化物岐化酶、过氧化氢酶、过氧化物酶活性的影响以及对脂质过氧化指标-丙二醛含量的影响,探讨了在Cd胁迫下,泥鳅肝组织内抗氧化酶活性以及脂质过氧化的变化趋势及其关系。

Nested PCR analysis and southern hybridization analysis showed that no polymorphism between H.villosa, Pm97034 and susceptible parent Wan7 107 was detected with the clones from 6VS arm, whereas three clones from H.villosa genome DNA: RH42, RH55, and RH66 showed polymorphism. RGA6 cloned from H.villosa genome DNA was characterized identity to NBS, and show high homology to resistance genes as RPM1 and RPP13 in Arabiadopsis, LRR19 in wheat, and I2C-1 in tomato.cDNA library constructed from T.aestivum-H.villosa translocation line Pm97034 was screened by hybridization with RGA6. Four positive cDNA clones were obtained. R3-2-2 showing 60-90% identity with eukaryotic sulfite oxidases contained an open reading frame of 647bp encoding 140 amino acid, and contained a conserved Moco-dimer domain in the ORF. R6-2-2 showed an ORF containing an Euk-porin domain of 279aa with 20-40% identity to the eukaryotic voltage-dependent anion channel proteins. R8-1-1 showed a complete ORF of 1810bp encoding 493aa with 80-90% identity to plant catalases. R9-1-1 showed an ORF containing a BTB/POZ conserved domain of 204aa with 30-70% identity to pox virus and zinc finger proteins. R3-2-2 and R9-1-1 were the first cDNA clones containingconserved domain of SO and POZ respectively isolated from wheat. R8-1-1 contained a complete ORF with 81% identity to Cat-3. Aspects of the role of R8-1-1 may be same with Cat-3, and it would offer the opportunity for improvement of stress tolerance of wheat.

以RGA6为探针,筛选用抗白粉病小麦—簇毛麦易位系Pm97034构建的cDNA文库,得到了4个阳性克隆。R3-2-2与动植物的亚硫酸氧化酶(sulfite oxidase,SO)有60~90%的同源性,长度为647bp,编码140个氨基酸,具有开放阅读框并含有保守域Moco-dimer.R6-2-2与真核生物的VDAC(voltage-dependent anion chennel)蛋白有20~40%的同源性,长度为1047bp,编码279aa,是一个不完整的开放阅读框,预测结构具有Euk-porin结构域。R8-1-1与植物中已经克隆的过氧化氢酶有80~90%的同源性,长度为1810bp,编码493aa,具有完整的开放阅读框和过氧化氢氧化酶保守域。R9-1-1与动植物的POZ(pox virus and zinc finger protein)蛋白有30~70%的同源性,长度为1446bp,具有开放阅读框和BTB/POZ保守域。R3-2-2与R9-1-1是首次从小麦中克隆到的具有SO和POZ保守域的cDNA序列。R8-1-1具有完整的开放阅读框,与玉米Cat-3基因具有81%同源性,预测R8-1-1可能具有与Cat-3类似的功能,可为转基因小麦抗逆育种提供新的基因。

It is Investigated that the effects of vitamin C and E on M. nipponense in different molting stages by measuring oxygen consumption rates, ammonia-N excretion rates and activities of antioxidant enzymes(SOD,CAT,GSH-Px,GST,GR) in three groups which are control group,Vc group andgroup.1. The oxygen consumption rates are high in stages D2 and A, but low in stage C.

设立三个试验组:对照组,V_C组(200mgV_C/kg)和V_C+V_E组(200mgV_C/kg+400mg V_E/kg),以上述分期结果为标准,测定了三个试验组的日本沼虾在蜕皮各时期的耗氧率,排氨率以及抗氧化酶(超氧化物歧化酶SOD,过氧化氢酶CAT,谷胱甘肽过氧化物酶GSH-P_X,谷胱甘肽转移酶GST,谷胱甘肽还原酶GR)活性的变化,结果如下: 1。

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