抗菌的

C1oning ofinsecticidal novel cry\' genes to Coleopteran pest tYom Bacillus lhuringiensisisolates will be beneficial fOr app1ication of pesticidal novel gene resources andwill lessen the gap betWeen domestic and abroad.

从Bt菌中分离克隆杀鞘翅目害虫的特异基因，将有效开发利用我国丰富的杀虫基因资源，缩小国内外在Bt杀虫基因基础研究领域中的差距，为工程菌和抗虫转基因植物的研制提供新的基因来源。

Gravid An. sinensis demonstrated significant negative taxis towards bacteria A4 and Aeromonas sp., as well as slight taxis to Enterobacter sp., implying that the three bacteria were not the dietary preference for An. sinensis. However, in light of the possibility of their stable colonization into mosquito's guts, these strains may serve as the host cells of larvicide genes and transgenic anopheline antimalarial genes.

中华按蚊产卵时对A4菌和气单胞菌具有明显的负趋性，而对肠杆菌仅具有微弱的趋向性，推测这3种细菌可能不是中华按蚊食物的优先选择，但具有在蚊中肠内较为稳定存活的可能性，因此更适合于作为蚊幼生物杀虫基因的寄主细胞和仿转基因按蚊抗疟基因的寄主细胞被应用。

We also studied the effect of several solutions for producing zoosporangium in Artificial culture condition, the result shows the hyphea culture with 0.1% KNO3 produced the most quantity of zoosporangiums.

将这8株内生细菌与烟草黑胫病菌共培，进一步测定拮抗效果结果显示YN-55、NN-3、YN-4和YN-10 4株拮抗效果最好，均在66％以上，并且这4种细菌均对孢子囊的产生有较强的抑制作用，而菌株发酵液对孢子囊的产生的抑制作用不明显。

Besides, The nodulation with else host experiment told us that NWGX035 could nodulate with melilotus suaveolens Ledeb, sophora alopecuroides Linn, lotus corniculatus Linn, sophora viciifolia Hance, trifolium repens Linn. The property of powerful salt, alkali, high temperature endurance and wide nodulation could confirm this strain was a particular strain. The nodulation mechanism and endurance of salt, alkali and high temperature mechanism need to be studied further.

另外，回接其它寄主植物的试验表明， NWGX035能与草木樨、黄化苦豆子、百脉根、狼牙刺和白三叶共生结瘤，其广泛的共生结瘤范围和强的耐盐、碱和抗高温特性可以证明该菌株为一株性状优良的菌株，其共生结瘤机制和耐盐碱机制需进一步研究。

Besides, The nodulation with else host experiment told us that NWGX035 could nodulate with melilotus suaveolens Ledeb, sophora alopecuroides Linn, lotus corniculatus Linn, sophora viciifolia Hance, trifolium repens Linn. The property of powerful salt, alkali, high temperature endurance and wide nodulation could confirm this strain was a particular strain.

另外，回接其它寄主植物的试验表明， NWGX035能与草木樨、黄化苦豆子、百脉根、狼牙刺和白三叶共生结瘤，其广泛的共生结瘤范围和强的耐盐、碱和抗(来源：A9fBC论文网www.abclunwen.com)高温特性可以证明该菌株为一株性状优良的菌株，其共生结瘤机制和耐盐碱机制需进一步研究。

By inoculated to tobacco plants, six strains could be re-isolated from the inoculated plants, indicating that they were endophytic bacteria in tobacco. Culture these eight bacteria and Phytophthora parasitica var. nicotianae together, the result showed four endophytic bacteria YN-55, NN-3, YN-4 and YN-10 have the best antagonistic effects.

将这8株内生细菌与烟草黑胫病菌共培，进一步测定拮抗效果结果显示YN-55、NN-3、YN-4和YN-10 4株拮抗效果最好，均在66％以上，并且这4种细菌均对孢子囊的产生有较强的抑制作用，而菌株发酵液对孢子囊的产生的抑制作用不明显。

All of these had shown that the induced resistance could be transmitted.Oligosaccharides had not shown any direct activity against Puccinia striifomis in vitro. This suggests that Oligosaccharides can elicit resistance to diseases of wheat, and are different from common pesticides.

不同浓度(25ppm、50ppm、100ppm)的5号提取物的体外抑菌试验表明，低聚糖对条锈菌没有明显的抑制作用，说明低聚糖不具有直接杀菌或抑菌作用，使小麦产生抗锈性的原因在于诱发寄主植物的抗病潜能，使植物本身产生对病原菌的抗性，这与普通杀菌剂显然不同。

The results of bioassay indicated: 1 The new compound (compound 6) and other three compounds (compound 1, 2 and 5) showed strong anti-HIV activity (EC50.064μg/ml), compound 3 and 4 showed less activity (EC50=6.18μg/ml and 12.85μg/ml respectively); Compound 5 and 6 showed weak cytotoxicity, and compound 1-4 showed moderate cytotoxicity; 2 Compound 5 and 6 were activated to all the four tested microbial; Compound 1, 2 and 13 were activated to all the three tested bacterial, but were unactivated to the tested fungi, Penicillium avellaneumUC-4376; One of the test bacterial, Staphylococcus aurues, seemed to be sensitive to all the eleven triterpenoids.

新化合物（化合物6）和化合物1、2、5具有强烈的体外抗HIV活性，其EC50值均小于0.064μg/ml，化合物3和4也具有抗HIV活性，但活性相对较弱，EC50值分别为6.18μg/ml和12.85μg/ml；化合物1-6都具有一定的细胞毒性，且化合物5和6的细胞毒性明显弱于化合物1-4的。 2）新化合物（化合物6）和化合物5对所有四种试验菌都有一定的活性；化合物1 、2和13对三种细菌均有一定的活性，但对真菌橙色青霉UC-4367（Penicillium avellaneum UC-4376）无活性；金黄色葡萄球菌对所有11个三萜都较为敏感。

For metabolites of strain SIPI-800, three active compounds were found, named as SIPI-800-1, 2 and 3 respectively, moreover SIPI-800-1 was new compound showing immunosuppressant activity and moderate anti- anaerobic activity; 2 and 3 were isoflavones with estrogenic activity, attracting activity to fungal zoospores, inhibiting alcohol dehydrogenase and aldehyde dehydrogenase I activities.

对SIPI-800菌株的代谢产物研究，分离出3个活性化合物，分别命名为：SIPI-800-1、2和3，其中1化合物为文献没有报道的新化合物，具有免疫抑制活性（1ug/ml浓度抑制NK细胞88.9%的活性）和弱的抗厌氧菌活性；2和3为异黄酮化合物，分别为Daidzein和Genisten的7甲基化衍生物，具有雌激素活性、强吸引真菌游动孢子、抑制乙醇或醛脱氢酶等活性。

Ethambutol is a first-line drug used for antituberculosis therapy. It is often used in combination with isoniazid, rifampin, pyrazinamide, and streptomycin. The action of EMB in antituberculosis mechanism is the destruction of cell wall of tubercle bacilli via binding the target molecule -- arabinosyl transferases. These enzymes are involved in the polymerization of the cell wall arabinan. Inhibition of arabinan synthesis by EMB results in the accumulation of mycolic acids, leading to cell death.The EMB-resistance of MTB is involved with the mutation of emb operon or the over expression of emb operon.

乙胺丁醇是和异烟肼、利福平、链霉素、吡嗪酰胺联合治疗结核病的一线抗结核药物，EMB对结核分枝杆菌的作用机制与分枝杆菌胞壁结构破坏有关，主要作用于靶分子阿拉伯糖基转移酶，抑制了阿拉伯糖基聚合入阿拉伯半乳聚糖，从而影响细胞壁分枝菌酸-阿拉伯半乳聚糖-肽聚糖复合物的形成，导致菌细胞的死亡。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失