抗菌的

A developmental focus was placed upon the intermediates of the drug such as cephalosporin,antihypertensive,antilipemic,antiulcer,antineoplastic,antiviral,antineuralgic,vitamin and amino acid.

重点发展第二、三、四代头孢菌素中间体、第三、四代喹诺酮类药物中间体、新型抗高血压药物中间体、新型抗高血脂药物中间体、抗溃疡药物中间体、抗癌药及抗病毒药物的中间体、中枢神经药物中间体、手性药物中间体、维生素及各种氨基酸。

The bacteriostatic effects, action mechanisms and antimicrobial spectrum of the strains were tested. After 5 days of culture, a bacteriostatic belt of 6.9-9.67 mm in width was observed on the culture plate, and the inhibitory activity maintained for 10 d. Prefiltration fermentation of the strains P1 and P5 could significantly inhibit conidial germination of the pathogen, with inhibitive rate ranging from 97.51% to 98.12%. The action might be realized by inducing hyphal deformity, bioplasm concentration, conidial germination inhibition and distorting germ tubes of conidia.

通过对其抑菌效果、机制和抗真菌谱进行测定，结果表明，P1、P5和葡萄灰霉病菌对峙培养5 d后，均产生明显的抑菌带，抑菌带宽度为6.90～9.67 mm，培养10 d后，仍然保持稳定的抑菌效果；P1、P5的发酵原液对葡萄灰霉病菌孢子萌发具有显著的抑制作用，抑制率为97.51%～98.12%；P1、P5对病菌的作用机制表现为使病菌菌丝畸形、原生质浓缩、菌丝断裂、抑制病菌分生孢子的萌发、导致孢子芽管扭曲。

The method of disc+ granule is a better way to identify resistance to take-all in wheat; TH1 is a good material for the breeding of resistance to take-all in wheat.

菌饼＋菌粒法是较佳的小麦抗全蚀病鉴定方法：TH1是一个很好的抗全蚀病育种材料。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Results:according to the outcome of test,168 cases with new bacteriological positive ptb were divided into negative reaction group(group 1),positive reaction group (group 2)and strong positive reaction group(group 3).after antituberculotic treatment for 6 months.the rates of sputum coversion of group 1,group 2 and group 3 were 76.92%,94.79% and 100% respectively.the mean sputum coversion time of group 1,group 2 and group 3 were 12.46 weeks 5.0 weeks and 3.0 weeks respectively and the rates of focus absorption of group 1 ,group 2 and group 3 were 61.54%.

结果：168例痰菌阳性初治的结核病患者，根据ppd试验的结果分为阴性组，阳性组，强阳性组。经过6个月抗痨治疗后，ⅰ组痰菌转阴率为76.92%，平均转阴时间为12.46周，病人疗程结束时，肺内病灶x线吸收占61.54%；ⅱ组痰菌转阴率为94.79%，平均转阴时间为5.0周，病人疗程结束时，肺内病灶x线吸收占86.46%；ⅲ组痰菌转阴率为100%，平均转阴时间为3.0周，病人疗程结束时，肺内病灶x线吸收占100%。

According to the ability of the field isolates of Gibberella zeae to grow on the PSA with varying carbendazim concentrations, three sensitivity levels of isolates were determined in vitro. The sensitive isolates could grow at 0.5 μg/ml, but could not grow at 100 μg/ml. The high resistant isloates could grow faster than R at 50 μg/ml, and also could grow at 100 μg/ml.No low resistant isolates, that could grow fast at 1.4 μg/ml but could not grow at 50 μg/ml, were found among the field isolates.

根据在0.5、1.4、50、100 μg/ml等不同浓度的含药PSA平板上能否生长，将玉蜀黍赤霉田间菌株对多菌灵的敏感性划分为：敏感、中抗和高抗等3个水平，其中S菌株在0.5 μg/ml浓度下能生长，但在≥1.4 μg/ml浓度下生长受到完全抑制；R菌株在1.4 μg/ml浓度下能快速生长，在50 μg/ml浓度下能缓慢生长，但在≥100 μg/ml浓度下不能生长；HR在≥100 μg/ml浓度下仍能生长。

The result indicates that there are one dominant and one recessive complementary gene that are effective against the Indian race 2E16. However, the monosomic analysis results indicated there was one resistant gene to the Indian race 2E16 and the gene was located on chromosome 3B. The current study shows that it is different from the known genes.

采用常规杂交分析方法，对铭贤169与阿夫杂交的F_1、F_2、BC_1代及其亲本进行抗性鉴定和统计分析，结果表明，阿夫对2E16菌系的抗性至少由1对显性抗条锈基因控制；通过单体分析将阿夫抗2E16菌系的1对显性基因定位在3B染色体上，等位性分析表明该基因不同于目前的已知基因。

Using Sarcina lutea as original strain,a ethionine and zinc chloride-resistant mutant was screened after treated with UV.

通过对谷胱甘肽新产生菌———藤黄八叠球菌进行紫外诱变处理，筛选到一株抗乙硫氨酸和氯化锌的抗性菌株，该突变株发酵生产谷胱甘肽的产量比出发菌株提高268.9%。

Identification of extensive phenotypic information to 6 pare cultures and detection of the mol% G+C ratio of the DNA to representative strains, showed that the examined bacteria belonged to a new morphovar of Acinetobacter junii, and was designated as Acinetobacter junii Morphovar Ⅰ. In addition, serotype, antibiotic sensitivity and pathogenicity of isolates were studied, the results showed that the 6 strains have the same K-antigen and O-antigen, there arc no obvious differences in sensitivity and resistance to used 37 antimicrobial agents between strains, and have strong pathogenicity to experimental stone bonder and Bastard halibut.

经对6株纯培养菌在形态特征、理化特性等方面较系统的表观分类学指征鉴定及代表菌株DNA中G+C mol%的测定，表明为琼氏不动杆菌的一个新形态型并定名为琼氏不动杆菌形态型Ⅰ(Acinetobacter junii morphovar Ⅰ)；同时，对该菌进行了血清型、对抗菌类药物的敏感性及致病作用等方面的试验，初步表明此6株菌具有同种的表面及同种的菌体抗原，对供试37种抗菌类药物在不同菌株间的敏感及耐药无明显差异，对供试石鲽及牙鲆均具有较强的致病作用。

A cluster analysis of two-hundred candidate wheat cultivars from Shaanxi, Gansu, Sichuan and Henan provinces recently was performed by using unweighted pair-group average method,based on the data from their seedling resistance reaction to six dominant isolates of Puccinia striiformis with the different virulence.

为了弄清我国小麦条锈病主要流行区后备小麦品种的抗锈性，选用6个条锈菌优势菌系，对陕，甘，川，豫四省近年来培育的200份后备小麦品种进行苗期和成株期鉴定，并分别对不同省份后备品种的抗条锈性进行聚类分析。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失