抗菌的

Evaluation of IEM103 in rabbit animal model.The evaluation was based on the control-challenge study (IEM101 as control). Rabbits were laparotomized, injecteded 10〓 cfu directly into proximal small bowel, and 28 days later, challenged with 0.25--2ug CT and 10〓-10〓 cfu 4 wild toxic strains with different biotypes and serotypes. Experimental group developed antitoxic and significant vibriocidal immune responses to vaccination, and could protect no less than 2ug CT and 10〓-10〓 cfu all wild strains challenge.

IEM103的免疫原性评价：经兔肠段结扎免疫〓cfu IEM103，7天后在外周血中可检测到外周淋巴细胞分泌性抗CT IgG，血清中可检测到抗CT抗体、杀弧菌抗体，P／N均≥2，且可持续至28天。28天后用0.25-2ug CT毒素，四种毒株滨-43，吴江-2，395，1119〓cfu菌量攻毒，实验组可保护至少2ug CT攻毒，而对照组可保护1ug以下CT攻毒，两组均可保护〓cfu四种毒株攻毒（以肠积液量≥1.0ml／cm为不保护判断标准）。

A set of spelt monosomic lines as female parent were respectively crossed with wheat new variety SM96 and new line SM20005 to determine the chromosomal location of the gene conferring resistance to powdery mildew in both. Seeding of parents, F〓 and F〓 from monosomic F〓 were inoculated with race no. 15 of Blumeria graminis f. sp. tritici. Analysis of obtained data revealed that one major dominant resistant gene originating from wild Emmer in the variety SM96 and the line SM20005 was located on chromosome 4A.

为确定小麦新品种沈免96和新品系沈免20005含有的抗白粉病基因所在染色体，以一套斯卑尔脱单体系为母本分别与沈免96和沈免20005杂交，将亲本、F〓、单体F〓植株的F〓代群体在苗期用小麦白粉菌15号小种的单孢菌系接种鉴定，单体分析结果表明，沈免96及沈免20005所含源于野生二粒小麦的抗小麦白粉菌15号小种的1个显性主效基因位于4A染色体上。

The effects of anti fatigue and oxygen deficient durable under normal atmospheric pressure of Fomes Officinalis Ames were observed in the fatigue test and oxygen deficient durable experiment in mice.

本研究采用抗应激实验的方法，观察新疆药用层孔菌对小鼠抗疲劳能力以及耐常压缺氧能力的影响，比较给药组与对照组小鼠存活的时间，对测试结果的方差分析及多重比较表明，药用层孔菌具有很好的补益作用，能增加小鼠的抗应激能力，同样条件下能延长小鼠的存活时

Isolates from watermelon-growing areas were classified into 3 races like race 0, race 1 and race 2 which were 8 (17.4), 30 (65.2), and 8 (17.4%), respectively. Pathogenecity of race 0 isolates are weakest which only make Sugar Baby susceptible; isolates of race 1 are intermediately pathogenetic, they make Sugar Baby and Charleston susceptible Gray but Calhoun Gray resistant; isolates belonging to race 2 possess the strongest pathogenecity, which make 3 hosts susceptible. Isolates of race 0 mainly distribute over the central and southern Hebei province, whereas race 2 cover the middle and north of Hebei, isolates of race 1 exist in the whole watermelon-growing areas in Hebei province.

根据鉴别寄主对供试菌系的抗感反应，将46个西瓜枯萎病菌系划分为0号、1号和2号3个不同的生理小种，分别包括8，30和8个菌系，占供试菌系的17.4%、65.2%和17.4%；0号生理小种菌系致病性最弱，仅使品种Sugar Baby感病，以冀中和冀南居多；1号生理小种菌系致病力中等，使鉴别寄主Sugar Baby和Charleston Gray感病，而使Calhoun Gray抗病，分布在整个河北省西瓜种植区；2号生理小种的菌系致病力最强，使3个鉴别寄主均能感病，主要分布在冀中和冀北。

We used Aeromonas hydrophila which we separated from the diseased Paralichthys olivaceus to made a inactivated whole cell bacterin .We injected this vaccine in the mice and the Paralichthys olivaceus .Both the mice and the Paralichthys olivaceus gained a good protection of 80% and 70%.The fish with dipping immersion gained a good protection of 85%,and the incidence of disease was 6%.We detected the antibody level in the antiserum by agglutination reaction and indirect ELISA which seemed to be immunogenic crucially.We anatomized the mice then we found that all the purtenance of the immunzied mice are healty.All of this proved that the vaccine is effective.

本实验将所分离到的嗜水气单胞菌用终浓度为0.2%~0.5%的甲醛对菌体进行灭活，制备了灭活疫苗，并优化灭活疫苗的灭活条件；将制备的灭活疫苗分别注射小鼠和牙鲆，均取得了较为理想的效果，其中小鼠的免疫保护率达到80%，牙鲆的免疫保护率达到70%；采用渗透压差法大规模浸泡免疫牙鲆也取得了很好的效果，浸泡免疫过的牙鲆发病率仅为6%，攻毒后免疫保护率为85%；通过应用间接ELISA方法检测受免动物的抗血清，并将牙鲆的抗血清注射新西兰白兔，从而获得兔抗鱼血清，更便于检测，结果都表明动物体内产生了较高的抗体水平；剖检攻毒后的动物，发现免疫过的动物内脏健康无异样，表明该疫苗安全有效，从而为防治牙鲆腹水症开辟了一条新途径，也为进一步制备其基因工程疫苗奠定了基础。

In order to find new kinds of antibacterial agents, a primary screening model has been set with the DNA gyrase B subunit as a target, and then the active compounds of positive strain were purified. From Mycobacterium phlei, we isolated a novobiocin supersensitive mutant M.phlei SS12 and a resistant ~mutant M.phlei RR11 with NTG and UV.

实验采用Mycobacteriumphlei为出发菌株，利用NTG和紫外线复合诱变得到新生霉素特异性的超敏菌株M.phleiSS12和耐药菌株M.phleiRR11，建立了以DNA回旋酶B亚基为靶点的抗结核药物的筛选模型；并应用此模型对1600株真菌和100株放线菌发酵液进行了初筛，获得了38株阳性菌，阳性率为2.4%；对阳性菌株3277的发酵液进行了提取分离，得到X1和X2两个活性化合物。

A bacteriocin-like substance producing strain T12 was screened from 212 lactic acid bacteria isolated from intestine of healthy pigs by paper disk test and was identified as Lactobacillus. The supernatant of T12 exhibited strong inhibitory activity against the gram-positive bacteria, including Micrococcus lysodeikticus, Staphylococcus aureus, Bacillus subtilis and Bacillus cereus. A strong activity was also observed against the gram–negative bacteria and some eumycete, such as Escherichia coli, Salmonella typhimurium and Fusarium graminearum.

通过滤纸片法从健康肉猪猪大肠、小肠中分离得到212株抗生物质产生菌，以杯碟法复筛，得到1株对溶壁微球菌、金黄色葡萄球菌、枯草芽孢杆菌、蜡状芽孢杆菌等革兰氏阳性菌和大肠杆菌、鼠伤寒沙门氏菌等革兰氏阴性菌以及部分真菌如禾谷镰刀霉均有强烈抑制作用的乳酸菌。

A fungus CTB430-1 with resistance to copper and zinc was isolated from soil at a copper and Zinc Tailing of Linxiang in Yueyang city, Hunan province. The fungus was identified by analyzing the morphology and measuring the ITS sequence. The effects of pH, initial concentration, co-ions on the growth of CTB430-1 and bioaccumulation of Cu2+ and Zn2+ were studied. The protein and glutathione in the cells were determined by using the coomassie brilliant blue method and iodometric method, respectively.

摘 要：从湖南省岳阳市临湘铜锌尾砂坝土壤内分离、纯化得到1株抗铜和锌的菌株CTB430-1，分析该菌株形态和测定菌株ITS基因序列鉴定菌种，研究不同pH值、金属离子初始浓度和金属离子共存对菌体生长量和富集重金属的影响；分别采用考马斯亮兰法和碘量法测定菌体内可溶性蛋白和还原性谷胱甘肽含量。

Methods After Candida albicans were inoculated into the specific pathogen free mice, the total amount of Candidas in cecum and the amount of which attached to the mucosal membrane were counted at different interent intervals. The lymphocyte proliferation in Peyer's patch and in lamina proper was shown by BrdU incorporation, meanwhile B cells isotype switch in PP was investigated. IgA plasma cells were shown by immunohistochemical staining. Specific IgA antibodies to Candida albicans were measured with ELISA.

采用无特殊病原菌动物经口喂入白念菌，在不同时相处死后，观察肠内白念菌总数及肠粘膜表面白念菌粘附数量；取肠系膜淋巴结做白念菌选择培养，观察移位体内发生率；采用Brdu体内掺入显示局部粘膜淋巴细胞的增殖情况；流式细胞计数潘伊尔氏结细胞表面IgA阳性率；免疫组化染色后计数固有层中IgA浆细胞数量变化；ELISA法测定肠粘液中特异抗白念菌IgA含量。

Indica variety ZYQ8 is an important genetic resource resistant to rice blast disease in rice breeding in North China.in this study,resistance identification of the populations of F1,F2,DH and B1F1of the crosses of ZYQ8×Jingxi17 (JX17) and ZYQ8×Lijiangxintuanheigu was carried out by using a Chinese differential strainZH10-8-14 from North China and a Japanese differential strain Ken54-04 from Japan and the results demonstrated that ZYQ8 had one dominant major gene Pi-zh which had been reported by Zhu L.H., et al., The results from experiments in which the F2 populations of ZYQ8×nine different varieties with known resistant genes were inoculated with strain Ken54-04 indicated that the gene Pi-zh was a new gene and non-allelic to nine known gene loci,namely Pi-i,Pi-km,Pi-z,Pi-ta,Pi-ta2,Pi-zt,Pi-kp,Pi-b and Pi-t.

籼稻品种窄叶青8号是我国北方稻区水稻育种上重要的稻瘟病抗源之一。本文利用我国北方稻区的代表菌系中10-8-14和日本的代表菌系研54-04，对窄叶青8号与感病品种京系17号和丽江新团黑谷的杂交F1、F2、DH和B1F1群体进行抗病性鉴定。根据抗病性的分离，确认窄叶青8号的抗性由1对显性主效基因，即朱立煌等报道的Pi-zh基因控制。利用菌系研54-04接种窄叶青8号与9个具有已知抗病基因的鉴别品种杂交的F2群体，各群体都表现二基因的独立遗传，证明Pi-zh基因与Pi-i、Pi-km|、Pi-z、Pi-ta、Pi-taz、Pi-zt、Pi-kp、Pi-b和Pi-t等9个已知抗病基因间存在非等位关系，是新的抗稻瘟病基因。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失