抗菌的

The results show that the synthetic compounds conform to the target compounds and the primary bioassay for anti-vibrio activity displays that the pyrrole alkaloids have a weak inhibition against Vibrio parahaemolyticus and Vibrio alginolyticus compared with the positive control of chloromycetin.

结果表明，所合成的化合物与目标化合物结构相一致。初步的抗溶藻弧菌和副溶血弧菌活性筛选表明，合成的吡咯生物碱具有一定的抗弧菌活性，但相对于阳性对照药氯霉素来说，合成的绝大部分吡咯生物碱抗弧菌活性较弱。

Comparison of reaction for resistance spectrum to 20 strains including 10 Philippine races, 3 Japanese races, and 7 Chinese pathotypes between CBB23(carrying Xa23 )and IRBB21(carrying Xa21) was made.

比较了CBB23 (携Xa23)和IRBB21 (携Xa21)对20个菌系包括10个菲律宾小种、3个日本小种和7个中国病原型代表菌系的抗谱， Xa23抗所有20 个菌系；Xa21 抗19个菌系，对菲律宾10号小种则高度感病。

Reaction of 24 Einkorn accessions to mixed inoculum of powdery mildew and stripe rust,and two T.urartu accessions UR204,UR206 and a T.monococcum accession MO205 were used for reaction to a set of 15 differential Blumeria graminis tritici isolates and 21 differential Puccinia striiformis isolates,respectively.Among them,UR206 was resistant to all 15 isolates,UR204 was resistant to 14 isolates except E11,and MO205 showed different resistant types.The results indicated that most likely UR206 and UR204 carried new powdery mildew resistance genes that were different from 18 known Pm genes and MO205 carried new stripe rust gene that were different from 24 known Yr genes.

本研究对24份一粒小麦分别进行了白粉病和条锈病混合菌种苗期接种鉴定，进一步分别用一套白粉病菌菌株(15个)对2份乌拉尔图小麦和条锈病菌小种(21个)对1份栽培一粒小麦进行接种鉴定，其中乌拉尔图小麦UR206能抵抗所有供试白粉菌菌株，UR204除对白粉菌菌株E11感病外，对其余菌株表现抗性；栽培一粒小麦MO205对不同条锈菌小种表现出不同的抗性反应，研究表明乌拉尔图小麦UR206、UR204和栽培一粒小麦MO205分别含有与已知抗白粉病和抗条锈病基因不同的新基因。

Theoptimal conditions were as follows: amidulin 1.25%,CaCO_3 0.05%, yeast0.6%,NaC1 0.7%,KH2PO4 0.3%. The stability of antagonistic substance produced by Q6 was studied. Theactivity of the inhibitive substance was influenced when time and temperaturewaxing. In 100℃for 30 minutes,the inhibitive zone diminished distinctly, but thesubstance still had inhibitive effect on the pathgen. In pH 3.0～above 9.5 forovernight or treated by Proteinase K, the inhibitive zone is not changed.

对其拮抗物质进行了温度、酸碱度和酶三个方面的稳定性试验，当温度达到55℃以上时，随着温度和时间的变大，抑菌圈逐渐变小，但直到100℃，30min时，对病原菌的生长仍有抑制作用；pH3.0～9.5以上静置过夜后，拮抗物质对病原指示菌产生的抑菌圈几乎没有发生变化；经过蛋白酶K处理后，拮抗物质对病原指示菌产生的抑菌圈不变。

Through researching the mechanism of biological nitrification and denitrification and actual project application of the technology and related literature materials, the obtained results are as follows:(1) Anoxybiosis is effective to the removal of COD; after the anoxic process, BOD/COD of the outflow increases, it shows that the degradation ability of wastewater is improved; anoxybiosis is insensitive when shock loading of inflow wastewater varies greatly;(2) It is demonstrated by the operation of inoculation and cultivating that biofilm takes two weeks to succeed in cultivating on packings and the removal ratios of NH3-N and COD get 50% and 70% respectively, when gas-water ratio is 6:1, HRT is 7.3 hour, water temperature is above 15°C;(3) It is demonstrated that gas-water ratio, hydraulic loading, HRT, temperature, pH etc are important factors which influence the removal effect of pollutants, when the gas-water ratio is 6:1, hydraulic loading is about 0.38kgNH3-N/m~3.d, water temperature ranges from 15°C to 27°C, pH ranges from 7.5 to 8.0, the BIOFOR removal effect of pollutants is the best;(4) The operation indicated that, this technology has strong ability of nitrification and denitrification; it has extremely vital significance for reducing the water body eutrophication;(5) It is confirmed the feasibility and the usability of preanoxic-BIOFOR process to treat L-lactic acid production wastewater. The experiment indicated that the treatment effect of this technology is very good and also shows that the technology has many advantages, such as small volume, high treatment efficiency, good effluent quality and strong endurance to load variation, this technology is a new and economical wastewater treatment process.

通过对生物脱氮机理的研究和该组合技术的实际工程应用，并结合有关文献资料，取得如下结论：(1)预缺氧池对L-乳酸生产废水中的COD去除具有一定的效果；废水中难降解的高分子物质经过缺氧工段分解为易于氧化降解的物质，浮状有机物在水解菌的作用下，将不溶性有机物水解为溶解性物质；预缺氧池具有较强的抗冲击负荷能力；(2)BIOFOR接种挂膜过程表明：HRT为7.3h、气水比为6：1、水温保持在15℃以上，经过半个月生物膜即以形成，NH_3-N和COD的去除率分别达到50％和70％左右；(3)研究表明：气水比、水力负荷、HRT、温度、pH等是影响污染物去除效率的重要因素，在气水比为6：1，NH_3-N容积负荷为0.38kgNH_3-N／m~3·d，温度在15℃-27℃，pH在7.5-8.0之间的条件下，BIOFOR对污染物的去除效率达到最佳；(4)运行表明，该组合技术具有较强的脱氮能力，对于减少水体富营养化具有十分重要的意义；(5)经过半年多实际工程的现场调试与运行，验证了利用预缺氧—BIOFOR组合工艺处理L—乳酸生产废水的可行性和实用性，得出该组合技术具有占地面积小，处理效果好，运行费用省，耐冲击负荷能力强等优点，是一项新型、经济的污水处理技术。

The results of in-door antagonism experiment of PSB3 to 12 strains of Fusarium and Colletotrichum showed that PSB3 had antagonism reaction against 6 of the 12 pathogens.

对该菌株与供试的12个炭疽菌和镰刀菌菌株进行室内拮抗试验，结果显示，该菌株对Fusarium solani等6个菌株有不同程度的拮抗作用。

TBA analysis showed 0.01% of TP had evident antioxidation on lipids from fish meat, and TBA increased with the TP concentration accretion presenting an unlinear relation. The effects of antioxidation of 0.03% and 0.07% of TP were close, thus, the best choice is 0.03% in practical application.5. 93 bacteria strains were isolated and categorized into several spoilage bacteria classes: Aeromonas, Vibrio, Pseudomonas, Enterobacteriaceae, Flavobacterium, Acinetobacter, Photobacteriaceae, Brevibacterium, and Micrococcus. In the early stage of storage (1～3 days), Gram-positive bacteria were the major strains and accounted for 70%, most of them belonged to Brevibacterium and Micrococcus.

TBA试验结果显示，添加00.1%以上茶多酚对草鱼脂肪都有明显的抗氧化作用，随浓度增加TBA值增加，但不呈线性关系，0.03%～0.07%抗氧化效果比较接近，从经济、有效的角度考虑在实际应用中可采用0.03%的茶多酚。5、从草鱼中分离出93株菌株，鉴定出以下几类主要腐败细菌：气单胞菌属、弧菌属、假单胞菌属、肠道杆菌科、黄杆菌属、邻单胞菌属、发光杆菌属、产碱杆菌属、短杆菌属、微球菌属、不动杆菌属、无色菌属。

Six experimental stages were designed in our procedure, those are:(1) metabolite recovery and tested sample preparation: the metabolites were recovered by Amberlite XAD-2 absorption, followed by MeOH elution and solvent concentration;(2) antioxidant detection and strain selection: samples were quantitatively analyzed by the inhibition effects on formation of lipid peroxides and TBARS to screen the strains able to produce antioxidants. According to the established screening methods, we chose out a strain of actinomycetes, designed as AMBL-029C;(3) antioxidant purification: the fermentation broth was recovered by a series of separation techniques including centrifugation, Amberlite XAD-2 absorption, followed by MeOH elution and a successive TLC purification. The resulting primary purified compound [temperately designed as AMBL-029C-TS] was further analyzed by HPLC to monitor its purity;(4) physical-chemical characteristics: judging from the acid-base fractionation experiments, and the pH and temperature stability tests, the compound was deduced to be a acidic compound with the properties of low polarity and highly pH and temperature stable;(5) mechanism of the antioxidant: in comparison with some other known antioxidants, TS was subjected to investigate its antioxidant mechanism, together with BHT,-tocopherol, as well as two streptomyces metabolites, homogentisic acid and -phenylpyruvic acid, which were previously isolated as the natural antioxidants in our laboratory.

针对本实验目的，我们设计了以下的实验步骤﹔（1）二次代谢物回收及检测样本处理：我们将发酵所得的培养上清液，利用疏水吸附性树酯Amberlite XAD-2吸附回收，并以甲醇溶离及真空减压浓缩脱水等方式处理，以取得提供抗氧化活性筛选之检测样本；（2）抗氧化活性检测及菌种筛选：以「过氧化脂质」和「硫丙二醯尿」的生成量进行定性定量分析以作为抗氧化物质生产菌筛选之用；经此筛选程序，我们选获了具有抗氧化物质高生产力的菌株，命名为AMBL-029C；（3）抗氧化物质的分离纯化：针对生产菌株的发酵回收处理液，以矽胶薄层色层分离法经物质层析纯化后，并以高效能液相层析法(High performance liquid chromatography；HPLC)分析物质可得一初级纯化物质，命名为AMBL-029C-TS；（4）抗氧化物质的物理化学性质分析：由酸碱转溶（acid-base fractionation）实验得知，此抗氧化物质属於中低极性的强酸性物质，对温度（37℃-100℃）及酸碱度（pH3.0-13.0）均表现出高稳定性；（5）在抗氧化机制探讨方面，我们针对数种不同的抗氧化机制进行探讨，即： 1。

METHODS: The different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal antikeratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal antikeratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育，倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数；将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育，分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

Keratin antibody IgM 3B4 specifically against Candida albicans in vitro. METHODS: The different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with Candida albicans yeast phase suspension on condition that profited germ tube formation of Candida albicans to observe the action of natural monoclonal anti??keratin antibody IgM 3B4 to Candida albicans germ tube formation.And the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti??

将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4分别与白念珠菌酵母相悬液在有利于芽管形成的条件下共同孵育，倒置显微镜下计数白念珠菌总数以及白念珠菌芽管数；将不同浓度的单克隆天然抗角蛋白抗体IgM 3B4与白念珠菌酵母相和人角质形成细胞混悬液、白念珠菌酵母相和人口腔黏膜上皮细胞混悬液、白念珠菌酵母相和胎儿脐静脉内皮细胞混悬液共同孵育，分别计数人角质形成细胞、人口腔黏膜上皮细胞、胎儿脐静脉内皮细胞上粘附的白念珠菌数。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失