抗菌法

The specific properties of materials, such as the palate adhesion, dynamic cruor, anti-bacterial and the degradability of lysozyme, were studied, which provide valuable data for further application. The main creative ideas are listed as follows:(1) The water-retention chitosan/poly vinyl alcohol blend fiber was obtained by coagulating in 10％ NaOH/ethanol solution, and the blend and crosslink due to GA can enhance the mechanical properties of fiber;(2) The controlled degradable chitosan/gelatin blend fiber was obtained by solution filature, and the mechanical properties were improved;(3) The N-acylchitosan with the different degree of substitution were successfully prepared, and the relationship between structure and properties was estabilished;(4) By a new solvent system of 6％ NaOH/4％ urea for cellulose, the blood anti-coagulant function films were prepared by blending cellulose with chitin;(5) In the same solvent system above mentioned, the anti-bacterial blend films based on cellulose and carboxymethyl-chitosan were prepared, and the relationship of DS and the anti-bacterial property was concluded;(6) The controlled degradable chitosan films was obtained by the chemical crosslink.

本论文的主要创新点：（1）以NaOH水溶液／无水乙醇为凝固液，采用溶液纺丝法制备出保水性壳聚糖／聚乙烯醇纤维，共混和戊二醛交联极大提高了壳聚糖纤维力学性能；（2）用壳聚糖和明胶共混，以溶液纺丝法制备出可控降解的壳聚糖／明胶纤维，并大幅度提高了壳聚糖共混纤维的干、湿态抗张强度；（3）确立了不同取代度N-酰基壳聚糖的合成方法及其膜的结构与性能的关系；（4）利用纤维素新溶剂6％NaOH／4％尿素制备出抗凝血性纤维素／甲壳素共混膜；（5）利用上述纤维素新溶剂制备出抗菌性纤维素／羧甲基壳聚糖共混膜，揭示了羧甲基壳聚糖取代度与抗菌性能的关系；（6）制备出可控降解壳聚糖交联膜。

The reasons for significant error were studied by adopting TTC chromogenic method, analyzing alcohol extract of natural antimicrobic component's polarity and making sample appropriate diffusion before plate cultivated.

采用氯化三苯四氮唑对指示菌显色、分析天然产物乙醇浸膏中抗菌成分的极性、样品在平板培养前适当扩散等方法，对K-B法检测天然抗菌成分存在显著误差的原因进行了研究。

Adopting duple diluent method and stiletto method and external antibacterial test on escherichia coli, solmonella paratyhpi , staphylicoccus aureus, streptococcus,, pseudomonas aeruginosa , Erysipelothrix rhusiopathiae, pasteurellosis to get the MIC and MBC, we studied antibacterial effect of the Copmounds Solatium Iyratum Thunb injection.

本研究采用试管二倍稀释法和打孔法对大肠杆菌、沙门氏菌、金黄色葡萄球菌、链球菌、绿脓杆菌、丹毒杆菌、巴氏杆菌的最小抑菌浓度和最小杀菌浓度及体外抑菌试验，研究复方白毛藤注射液抗菌作用。

Based on phenotypical and biochemical properties as well as its 16S rRNA gene sequence, the bacterium was identified as Paenibacillus polymyxa. In vitro antifungal spectrum assay vindicate that it was active against a broad range of phytopathogens and inhibited the growth of thirteen fungal pathogens.

采用对峙培养法筛选拮抗内生细菌，根据菌株形态、生理生化特性结合16S rRNA基因序列分析鉴定菌株；采用硫酸铵沉淀法提取抗菌粗蛋白，研究其对水稻恶苗病菌菌丝生长和孢子萌发的影响。

The antimicrobial activities were determined with paper disc diffusion method. The result showed that of Sapindus mukorossi extracts only inhibited Candida albicans and no antimicrobial activities to Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Pityrosporum ovale and Propionibacterium acnes.

配制无患子溶液进行抗菌试验，以圆盘滤纸扩散法分析，结果显示甲、乙、丙、丁醇溶液样品，唯对白色念珠菌具抑制效果，而对另外5种菌种(大肠杆菌、金黄色葡萄球菌、绿脓杆菌、皮屑芽孢菌、痤疮杆菌)无明显抗菌效果。

objective in order to exploit the natural resources of chinese herbs,the inhibitive effects of the compositions of28chinese herbs on the growth of8common bacteria in oral cavity were determined.methods sen-sitive test of these bacteria were carried out by mtt method.at the same time,chessboard dilution method was used for combined sensitive test.results the compositions of28chinese herbs can inhibit the growth of5bacteria in dif-ferent levels,but the effects on other3bacteria are not obvious.quercetin and usnic acid have better effects on5bacteria than other26herbs compositions,with mic0.03125～0.125mg/ml and0.0156～0.0625mg/ml.conclu-sion quercetin and usnic acid can inhibit the5bacteria growth more effectively than other26herbs compositions.

目的 测试28种中药有效成分抑制8种口腔常见致病菌的作用，为抗菌中药有效成分用于口腔感染性疾病的防治做一些探讨。方法选择28种有抗菌作用的中药的有效成分，采用噻唑蓝法对8种口腔常见致病菌作药物敏感实验，同时采用液体棋盘稀释法进行联合药敏实验。结果 28种中药有效成分对3种牙周细菌抑制作用不明显，对另5种口腔常见细菌有一定的抑制作用，其中松萝酸和槲皮素有较强的抑菌作用，mic值分别在0.0156～0.0625mg/ml和0.3125～0.125mg/ml之间。结论所选28种中药成份中，松萝酸和槲皮素具有较强的抑菌作用。

The cDNA squence of antibiotic, an antibacterial peptide, a process for preparing the said cDNA and a process for recombining the antibacterial peptide are disclosed. The present frequency of amino acid codens in Attacin A cDNA is analyzed. The high-frequency coden is introdued to its protein sequence to configure its cDNA sequence. The target gene is synthesized by chemical synthesis method or polymerase chain reaction.

本发明公开了葛佬素的cDNA序列，葛佬素cDNA的制备方法以及重组抗菌肽葛佬素的制备方法；通过对同种已知蛋白质Attacin A cDNA中氨基酸密码子的出现频度进行分析，采用出现频度高的密码子带入葛佬素蛋白质序列中进行葛佬素的cDNA序列构建，用化学合成法及聚合酶链式反应进行目的基因的合成，另外，采用基因重组法制备抗菌肽葛佬素，并测定了其活性。

The paper used the method of paper papilioform in the microbiology to determine the antimicrobial cycle diameter of the mixed preservaties of different formul as that are composed of natural extraction with antimicrobial action, humectants, emulsifjers, preservatires and acidity regulators, and that of single preservative. The mixed formulas with strong antimicrbial power and big antibiogram were elected.

采用微生物学中的纸蝶法，、测定由具有抗菌作用的天然抽提物、水分保持剂、乳化剂、防腐剂以及酸度调节剂组成的不同配方的复配型防腐剂与单一型防腐剂的抑菌圈直径，筛选出抗菌力强、抗菌谱广的复配型配方。

Lysozymes were immobilized on wool fabrics by sol-gel method in order to enhance the antibacterial function of wool.

为了增强羊毛织物的抗菌性，采用溶胶－凝胶法将溶菌酶固定在羊毛织物上，以期获得良好的抗菌效果。

So it was primarily identified as Bacillus subtilis. In the antifungal activity test,the method of sitzkrieg culture, asynchronism culture and hyphal extension inhibition assay of plate were adopted to study the inhibitive effect of the antagonist on Fusarium graminearum, and at the same time,the test conditions were explored.

在拮抗物质抗菌活性测定的研究中，分别采用对峙培养法、异步培养法和杯碟测定法研究枯草芽孢杆菌对小麦赤霉菌的抑制作用，探索抗菌活性测定的条件。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。