抗菌

A set of spelt monosomic lines as female parent were respectively crossed with wheat new variety SM96 and new line SM20005 to determine the chromosomal location of the gene conferring resistance to powdery mildew in both. Seeding of parents, F〓 and F〓 from monosomic F〓 were inoculated with race no. 15 of Blumeria graminis f. sp. tritici. Analysis of obtained data revealed that one major dominant resistant gene originating from wild Emmer in the variety SM96 and the line SM20005 was located on chromosome 4A.

为确定小麦新品种沈免96和新品系沈免20005含有的抗白粉病基因所在染色体，以一套斯卑尔脱单体系为母本分别与沈免96和沈免20005杂交，将亲本、F〓、单体F〓植株的F〓代群体在苗期用小麦白粉菌15号小种的单孢菌系接种鉴定，单体分析结果表明，沈免96及沈免20005所含源于野生二粒小麦的抗小麦白粉菌15号小种的1个显性主效基因位于4A染色体上。

Through Disc diffusion method,we finally selected 8 strains against Banana Fusarium wilt disease No.4 race.

通过纸片扩散法，从这139株拮抗放线菌菌株中，最终筛选对香蕉枯萎病生理小种4号具有拮抗作用的8个菌株。

For observing the resistance of clinically isolated gonococcus and the standard bacterial strain to chemical disinfectants, standard strain with relatively high resistance was selected to carry out organic interfering substance test.

观察临床分离的淋球菌和标准菌株对化学消毒剂的抗力，选择抗力较高的标准菌株作有机干扰物试验。

And the different concentrations of natural monoclonal anti-keratin antibody IgM 3B4 were incubated with the mixed suspenions of Candida albicans yeast phase with malpighian cells, human buccal epithelial cells, endothelial cells of fetal umbilical vein, respectively, to observe the action of natural monoclonal anti-keratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.

结果：与对照组比较，不同浓度的单克隆天然抗角蛋白抗体IgM3B4均能抑制白念珠菌芽管生成，并且抗体浓度越高，抑制白念珠菌芽管形成的作用越明显；同时单克隆天然抗角蛋白抗体IgM3B4能够显著减少白念珠菌与角质形成细胞、上皮细胞、内皮细胞的粘附，其抑制作用与单克隆天然抗角蛋白抗体IgM3B4的浓度呈正相关。

Keratin antibody IgM 3B4 to the adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein. RESULTS: It was found that the different concentrations of natural monoclonal anti??keratin antibody IgM 3B4 could inhibit Candida albicans germ tube formation and adhesion of Candida albicans to malpighian cells, human buccal epithelial cells and endothelial cells of fetal umbilical vein.Its inhibitory action was positively related to the level of natural monoclonal anti??

结果：与对照组比较，不同浓度的单克隆天然抗角蛋白抗体IgM 3B4均能抑制白念珠菌芽管生成，并且抗体浓度越高，抑制白念珠菌芽管形成的作用越明显；同时单克隆天然抗角蛋白抗体IgM 3B4能够显著减少白念珠菌与角质形成细胞、上皮细胞、内皮细胞的粘附，其抑制作用与单克隆天然抗角蛋白抗体IgM 3B4的浓度呈正相关。

Bacillus subtilis str. B5423, Pseudomonas fluorescens str. Pf7-14, nonfluorescent pseudomonads str. P13 are antagonistic to rice sheath blight. Resistance to antibiotic is the character that was chosen to use to mark the strains in order to monitor the population dynamics of the strain on the surface of rice plant and to elucidate the mechanism of suppressing the diseases.

中文题名拮抗细菌的生态学及其对水稻纹枯病的抑制作用副题名外文题名 Ecology of biological control agents and their suppressive effect on rice sheath blight 论文作者李湘民导师许志刚教授苗东华博士学科专业植物病理学研究领域\研究方向植物病害生物防治学位级别博士学位授予单位南京农业大学学位授予日期2002 论文页码总数116页关键词纹枯病拮抗细菌水稻馆藏号BSLW /2003 /S435 /19 Bacillus subtilis B5423，Pseudomonas fluorescens Pf7-14，和非荧光假单胞肝菌Pseudomonas sp.P13是对水稻纹枯病菌有拮抗作用的三支细菌菌株。

The antibiosis of Agricultural Antibiotic 120 (AA120), a product of Streptomyces hygrospinosus var. beijingensis, on Pellicularia sasakii was observed with the plate culture method.

采用平板培养法测定农抗120对水稻纹桔菌的抗生试验结果，表明农抗120对水稻纹枯病菌的抗生作用为抑制菌丝生长，使菌丝畸变。

The result of the antibacterial and antifungal assay shows that thirty five strains of the endofungi (58.3% of total tested strains) have antibacterial activities, the active strains mostly belong to sterile groups (51.4% of active strains), Penicillium (8.6%), Aspergillus (8.6%), Fusarium(8.6%); forty nine strains (81.7% of total tested strains) have antifungal activites, most of them belong to sterile groups (44.9% of active strains), and Gloeosporium (17.1%).

结果 共有35株内生真菌具有抗细菌活性，占待侧菌株总数的58.3%，其中以不产孢类、青霉属、曲霉属、镰孢属为主，分别占活性菌株总数的 51.4%、8.6%、8.6%、8.6%；共有49株内生真菌具有抗真菌活性，占待侧菌株总数的81.7%，其中以不产孢类和盘长孢属为主，分别占活性菌株总数的44.9%、17.1%。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

Biocontrol may be an alternative to avoid these problems in tobacco disease control. Ata28 strain is an antagonistic bacterium against tobacco brown spot was isolated from healthy tobacco in Chongqing.

Ata28菌株是从重庆烟区烟草健康植株上分离获得的烟草赤星病拮抗细菌，在PDA平板上可明显抑制烟草赤星病强致病力菌株Tbs3的生长，温室控病效果较好；为了进一步提高拮抗细菌Ata28菌株对烟草赤星病的防治效果，本研究采用紫外线、紫外线和氯化锂、硫酸二已酯及微波对Ata28菌株进行诱变选育，以期获得拮抗性能更强，生物学性状更好的优良菌株，为大田使用提供理论依据和实践经验。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失