抗结核的

Results Irritative symptoms (78%) and gross hematuria (36%) were the most common symptoms encountered in these patients.

结果尿路刺激症状和血尿为最常见症状，分别为78％和36％。24小时尿抗酸杆菌和尿结核PCR的阳性率分别为25％和40％，IVU，逆行尿路造影及CT的诊断符合率分别为38％，63％和44％。33例患者接受了肾及部分输尿管切除术，肾上盏结核病灶清除术1例，对侧输尿管膀胱吻合术1例及肾穿刺造瘘术1例。1例患者并发血行播散型结核死亡。

Results Irritative symptoms (78%) and gross hematuria (36%) were the most common symptoms encountered in these patients.Acid-fast stains on urinary sediment were positive in 25% of cases and TB-PCR was positive in 40%.The diagnostic accuracy of IVU,retrograde urography and CT in these patients were 38%,63% and 44% respectively.33 patients underwent a nephrectomy and partial ureterectomy,one of them had a contralateral ureter reimplantation.One patients died of miliary tuberculosis. Conclusions Acid-fast stain remains a major pathogen-detecting tool and the value of TB-PCR needs to be confirmed.

结果 尿路刺激症状和血尿为最常见症状，分别为78%和36％。24小时尿抗酸杆菌和尿结核 PCR 的阳性率分别为25％和40％， IVU ，逆行尿路造影及 CT 的诊断符合率分别为38%，63%和44%。33例患者接受了肾及部分输尿管切除术，肾上盏结核病灶清除术1例，对侧输尿管膀胱吻合术1例及肾穿刺造瘘术1例。1例患者并发血行播散型结核死亡。

In order to find new kinds of antibacterial agents, a primary screening model has been set with the DNA gyrase B subunit as a target, and then the active compounds of positive strain were purified. From Mycobacterium phlei, we isolated a novobiocin supersensitive mutant M.phlei SS12 and a resistant ~mutant M.phlei RR11 with NTG and UV.

实验采用Mycobacteriumphlei为出发菌株，利用NTG和紫外线复合诱变得到新生霉素特异性的超敏菌株M.phleiSS12和耐药菌株M.phleiRR11，建立了以DNA回旋酶B亚基为靶点的抗结核药物的筛选模型；并应用此模型对1600株真菌和100株放线菌发酵液进行了初筛，获得了38株阳性菌，阳性率为2.4%；对阳性菌株3277的发酵液进行了提取分离，得到X1和X2两个活性化合物。

The results demonstrated that 22 of 50 were positive by PCR-ELISA method while only 7/50 and 18/50 by antiacid staining and culture respectively, and no positive result was found in 20 sputum specimens without Mycobacterium tuberculosis by the methods; Conclusion The pCR-ELISA method can objectively detect Mycobacterium tuberculosis from clinical specimens with high specificity and sensitivity.

对50份来自结核病人高度怀疑有结核分枝杆菌的标本检测表明，PCR-ELISA检出22份阳性，比抗酸染色法(7/50)、培养法(13/50)和PCR-电泳法(18/50)检出率高，而20份证实无结核分枝杆菌的标本，几种方法检查均为阴性。结论该法可敏感、特异和客观地检测临床标本中的结核分枝杆菌。

Methods A total of 125 tuberculous patients with proteinic malnutrition, who were hospitalized in our department for initial treatment of pulmonary tuberculosis from September 2002 to May 2006, were studied for their hepatitis induced by the first line antituberculous drug.

对2002年9月－2006年5月住院治疗，合并蛋白质营养不良的125例初治肺结核患者，采用标准规化抗结核药物治疗方案，观察药物性肝损害情况。并以同期住院治疗的不合并蛋白质营养不良的初治肺结核患者769例为对照。

The suptum samples from 102 tuberculosis patients and 45 non-tuberculosis patients were detected by fluorescence quantitative pcr and acid-fast stain.

对102例培养涂阳的结核痰液标本和45例非结核感染者的痰标本，应用荧光定量pcr法、痰涂片抗酸染色法检测结核分枝杆菌。

Conclusions］There is a significant difference at protein level between untreated and ethambutol treated mc2155 cells. It suggests that the differential expression analysis of proteomes may be useful for further study on microbiostatic mechanism of ethambutol and search for new targets toward anti-TB drug development.

结论］乙胺丁醇处理前后耻垢分枝杆菌mc2155细胞的蛋白质组具有差异，这种蛋白质组的差异分析有助于进一步研究乙胺丁醇抑制分枝杆菌生长的作用机制并为寻找新的抗结核药物作用靶标奠定基础。

［Objective］To investigate the microbiostatic mechanism of ethambutol, proteomic methods were used to find differential expression proteins in Mycobacterium smegmatis mc2155 cells treated with ethambutol.

［目的］利用蛋白质组学方法，识别乙胺丁醇处理前后耻垢分枝杆菌mc2155细胞后差异表达的蛋白质，为进一步探讨乙胺丁醇抑制分枝杆菌生长的作用机制及寻找新的抗结核药物作用靶标奠定基础。

Results Various side effects resulting from antituberculous agents were confirmed in 302 cases (32.7%),liver injury,acoustic nerve injury, blood system injury, gastrointestinal response of them were primary (82.1%).Stopped treatment rate was 14.6%.77% in side effects occurred within 2 months after administration, but the blood system injury occurred after 3 months.

结果：抗结核药物副作用302例(32.7%)，以肝损害、位听神经损害、血液学异常及胃肠反应为主(82.1%)，因副作用致终止治疗率14.6%。77%的副作用出现于给药的2个月内，血液学异常的50%以上在用药的3个月后出现。

Results Various side effects resulting from antituberculous agents were confirmed in 302 cases (32.7%),liver injury,acoustic nerve injury, blood system injury, gastrointestinal response of them were primary (82.1%).

结果：抗结核药物副作用302例(32.7%)，以肝损害、位听神经损害、血液学异常及胃肠反应为主(82.1%)，因副作用致终止治疗率14.6%。77%的副作用出现于给药的2个月内，血液学异常的50%以上在用药的3个月后出现。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。