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The results show that the dispersity of emulsion is good and the average diameter of particles is about 15 nm. Its bactericidal properties to some bacteria and epiphytes are strong under daylight lamp irradiation. The killing rate to Gram negative bacterium (Staphylococcus aureus and Bacillus subtilis) is 100%, in addition, killing activity of it to Gram positive bacterium is very high. It has a clear antibacterial circle of about 20 mm in diameter toward epiphytes such as Aspergillus niger, Aspergillus flavus and Candida albicans etc.

结果表明,混晶乳液分散性好,粒径约15nm;在普通日光灯照射下,乳液对一些细菌和真菌有很好的抗菌性能:对革兰氏阳性菌(金黄葡萄球菌和枯草芽孢杆菌)的杀灭率达到100%,对革兰氏阴性菌也有很好的杀灭性能,对黑曲霉、黄曲霉和白色念珠菌等真菌产生清晰的抑菌圈,抑菌直径约20mm。

The result shows that 4.1-6.5 kDa antibacterial substances separated from the crude extract described above could efficiently kill Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis and Micrococcus luteus), but no any activity for Gram--negative bacteria or fungi.

可产生出分子量在4.1-6.5KD之间的抗菌物质,这些抗菌物质对革兰氏阳性菌(金黄色葡萄球菌、藤黄微球菌及枯草芽孢杆菌)有广泛的抗性,而对革兰氏阴性菌和真菌没有抗性。

nodular dermal vasculitis in lower extremities are often seen clinically in middle-age women, and are evoked by many causes. Some laboratory examinations have abnormal outcomes and we think these are related with autoimmune reactions of nodular dermal vasculitis. We can make diagnosis with clinical character of erythema and nodose lesions in lower extremities, laboratory examinations and histopathology inspection. Antibiotics, non- Steroid anti-inflammatory drugs, anti-tubercle drugs and corticosteroids are effective drugs.

结节型皮肤血管炎多发于中年女性;结节型皮肤血管炎病因复杂,可能是由于细菌、真菌、病毒、结核、药物等所引起,也可为某些全身性疾病的皮肤表现;实验室指标异常改变可能与疾病免疫反应有关;结节型皮肤血管炎诊断以双下肢红斑结节性损害的临床特点,结合实验室及组织病理学检查可确诊分型;结节型皮肤血管炎针对病因选用抗生素、非甾体抗炎药、抗结核药、皮质类固醇激素等治疗有效。

Drugs (methimazole, methylthiouracil, propylthiouracil and thiouracil); some sedatives (doxylamine succinate and phenobarbital); and some other drugs including the systemic antifungal antibiotic griseofulvin, the diuretic spironolactone, and the antibacterial sulfa drugs sulfamethazine and sulfamethoxazole.

抗甲状腺?药物(他巴唑,甲基硫氧嘧啶,丙基硫氧和硫氧嘧啶);一些镇静剂(doxylamine琥珀和苯巴比妥),以及包括系统性真菌抗生素灰黄霉素,安体舒通的利尿一些其他药物,以及抗菌磺胺类药物和磺胺二甲嘧啶。

Magainin is the skin secretion product of Batrachia under the eriviroment pressure, which can be used to accelerate the fusion rate of wound and resist the infection. It can inhibit the growth of bacteria and fungus with the lowest concentration; Protegrin is isolated from porcine leukocyte hi 1993 and is useful as antiviral agents.

Magainins是爪蟾的皮肤在一定的环境压力下分泌出的抗感染和促进伤口愈合的成分,低浓度便可抑制许多细菌和真菌生长;Protegrin是1993年从猪的中性白细胞中分离得到,研究发现它具有抗爱滋病毒的功能。

By using the traditional taxonomic methods, electronic microscope examination, 18S rDNA, ITS sequences and phylogenetic analysis, an endophytic fungus HCCB1621 isolated from the cephalotaxus sinensi was identified as Trichoderma viride Pers.ex Fr. Its anti-tumor activities were studied as well.

利用传统分类学方法、电子显微镜观察、18S rDNA和ITS序列的测定以及系统发育分析对一株分离自粗榧抗肿瘤活性内生真菌HCCB1621进行分类鉴定,并对其产物抗肿瘤活性进行了初步研究。

The antimicrobial test and researched was done on wide distillation of different solvents on Terpenoid, polarity separation of ethanol distillable substance, thin layer chromatography of different polarities and distillation of amylase. The results showed that the active substance distilled by 95% ethanol and V methanol: toluene=3:1 had stronger antimicrobial activity on Agaricus blazei Murrill. The antimicrobial activity of ethyl acetate was stronger than wide distilling substance with ethanol, but the water part has not antimicrobial effect. Two straps of ethyl acetate phase(Rf0.57、Rf0.63)(0.1g/ml)had stronger antimicrobial activity on Agaricus blazei Murrill, the antimicrobial zone on Pseudomonas was 19.3mm. The bacteriostatic activity of chromatography strap of water phase was rather weaker, the water solution (0.5g/g) of amylase had better effect on pathogenic fungus of Agaricus blazei Murrill, its antimicrobial zone on Aspergillus niger, Citrinin, Trichoderma viride were 10.8 mm, 7.9 mm, 5.3 mm.

通过对日本凹顶藻以不同溶剂的粗提、乙醇提取物的极性分离、不同极性的薄层层析和多糖的提取,进行抗菌试验的研究,发现95%乙醇和V甲醇:甲苯为3∶1的提取的活性物质具有较强的抗姬松茸的病原细菌活性,乙酸乙酯相对病原细菌的抑菌强于乙醇的粗提物,而水相基本无抑菌效果,乙酸乙酯相的两个带(Rf0.57、Rf0.63)浓度为0.1g/ml具有较强抗姬松茸病原细菌的活性,对假单孢杆菌抑菌圈达到19.3mm,水相层析带的抑菌活性较弱,多糖的粗提物配成0.25g/g的水溶液对姬松茸病原真菌有较好的抑菌效果对黑曲霉、桔青霉和绿色木霉的抑菌圈分别达到10.8 mm、7.9 mm、5.3 mm。

The study of the biological characteristics of resistant phytopathogenic fungi has great theoretic and practical significance for improving the strategy of chemical pesticide application, postponing the occurrence of the resistance and advancing the chemical prevention and control of diseases caused by phytopathogenic fungi.

研究植物病原真菌抗药菌的生物学特征以及它们的抗药机制对于改进化学农药使用策略,延缓抗药性的产生,推进植物病害的化学防治,具有十分重要的理论和实践意义。

The results of bioassay indicated: 1 The new compound (compound 6) and other three compounds (compound 1, 2 and 5) showed strong anti-HIV activity (EC50.064μg/ml), compound 3 and 4 showed less activity (EC50=6.18μg/ml and 12.85μg/ml respectively); Compound 5 and 6 showed weak cytotoxicity, and compound 1-4 showed moderate cytotoxicity; 2 Compound 5 and 6 were activated to all the four tested microbial; Compound 1, 2 and 13 were activated to all the three tested bacterial, but were unactivated to the tested fungi, Penicillium avellaneumUC-4376; One of the test bacterial, Staphylococcus aurues, seemed to be sensitive to all the eleven triterpenoids.

新化合物(化合物6)和化合物1、2、5具有强烈的体外抗HIV活性,其EC50值均小于0.064μg/ml,化合物3和4也具有抗HIV活性,但活性相对较弱,EC50值分别为6.18μg/ml和12.85μg/ml;化合物1-6都具有一定的细胞毒性,且化合物5和6的细胞毒性明显弱于化合物1-4的。 2)新化合物(化合物6)和化合物5对所有四种试验菌都有一定的活性;化合物1 、2和13对三种细菌均有一定的活性,但对真菌橙色青霉UC-4367(Penicillium avellaneum UC-4376)无活性;金黄色葡萄球菌对所有11个三萜都较为敏感。

The bioassay results showed that the quaternary ammonium salt had a good intihtion against typical microorganisms such as heterotrophic bacteria,ammonifying bacteria,denitrifying bacteria and nitrosation bacteria with its inhibition rate was above 90%. Inhibition of the quaternary ammonium salt to fungi and sulfate reducting bacteria was above 85% after 30 min. Inhibition of the quaternary ammonium salt to black mold was about 84% after 30 min,and the to algae was totally inhibited after 24 h.

高分子抗菌杀生剂的抗菌灭藻性能的检测结果表明,作用30 min对贵阳市雅河水中的异养菌、氨化细菌、反硝化细菌、亚硝化细菌的杀菌率在90%以上,真菌、硫酸盐还原菌的杀菌率在85%以上;对黑曲霉的杀菌率在84%,其作用24 h可将小球藻基本杀灭。

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