抗病

The main results are as following:(1) Arabidopsis in planta transformation were made by Agrobacterium tumefaciens carrying the activation tagging vector pSKI015 with herbicide-resistant Bar gene as a selective marker. About 12 000 independent transformant lines were acquired by the above method, from which two mutants related to plant defense response and development, asr1 (auxin and .salicylic acid responsive 1.) and dai1 (brought tolerant and obscisic acid insensitive 1) were isolated.

本文以拟南芥为实验材料，以激发标签为技术平台，构建了拟南芥的功能获得突变体库，从中筛选具有抗病、抗逆表型的突变体，克隆基因并进行基因的功能研究，取得的主要结果如下：(1)利用含激发标签质粒pSK1015的农杆菌直接转化拟南芥植株，以抗除草剂的Bar基因为筛选标记，获得了约有12000个独立转化株系的突变体库，并从中筛选到2个与抗病、抗逆和发育相关的突变体asr1(auxin and salicylic acid responsive 1)和dai1(drought tolerant and abscisic acid insensitive 1)。

The main work of this dissertation is constructing Arabidopsis gain of function mutants pool, screening mutants related to plant defense response, cloning the genes and studying their functions. The main results are as following:(1) Arabidopsis in planta transformation were made by Agrobacterium tumefaciens carrying the activation tagging vector pSKI015 with herbicide-resistant Bar gene as a selective marker. About 12000 independent transformant lines were acquired by the above method from which two mutants related to plant defense response and development, asr1 (auxin and salicylic acid responsive 1) and dai1 (drought tolerant and abscisic acid insensitive 1) were isolated.

本文以拟南芥为实验材料，以激发标签为技术平台，构建了拟南芥的功能获得突变体库，从中筛选具有抗病、抗逆表型的突变体，克隆基因并进行基因的功能研究，取得的主要结果如下：(1)利用含激发标签质粒pSKI015的农杆菌直接转化拟南芥植株，以抗除草剂的Bar基因为筛选标记，获得了约有12000个独立转化株系的突变体库，并从中筛选到2个与抗病、抗逆和发育相关的突变体asrl(auxin and salicylic acid responsive l)和dail(dought tolerant and abscisic acidinsensitive l)。

The resistance to disease of transgenic plants were tested by inoculating leaves with Puccinia coronata var.coronata cummins.

对所得到的转基因草地早熟禾植株人工接种冠锈菌夏孢子进行抗病性鉴定并测定了抗病性相关指标。

In addition, the hypha could also invade the rice root.3、Making clear the effects of silicon on the resistance to sheath blight and rice growth.Sodium silicate was added into the nourishing in the entire growth period and short period of rice, after being inoculated with R.solani, the rice with application (the Si+ rice) of resistant cultivar 91SP and Teqing had lower (but not statistically significant) disease rating and disease index with relative control effect of 10.02% and 8.70% compared to the Si- rice plants in, but the Si+ rice plants of susceptible cultivar Lemont and Ningjing 1 had significantly lower rating and disease index with relative control effect of 27.42% and 24.29% compared to the Si- rice plants. The results indicated silicon could strengthened the resistance to rice sheath blight, specially strengthened the resistance of susceptible cultivar Lemont and Ningjing 1 remarkedly.

纹枯病菌也能侵染水稻根部。3、明确了施硅对纹枯病的抗病效应和水稻生长的影响全生育期和短期在营养液中添加硅水培水稻试验结果表明，施硅减轻了病级和病情指数，施硅对抗病品种91SP和特青的相对防治效果分别为10.02%和8.70%，而对感病品种Lemont和宁粳1号的相对防治效果达27.42%和24.29%，说明施硅增强了水稻对纹枯病的抗性，特别显著增强了感病品种Lemont和宁粳1号的抗性。

Host resistance has been an elusive goal of many control programs. Most Sclerotinia diseases are not controlled principally by host resistance. However, some moderate levels of host resistance such as in dry beans and soybean have been found and can aid in integrated control programs.

在许多疾病控制程序中，获取植株的抗病性仍是一项艰巨的目标，大多数菌核病都不是由寄主的抗病性控制，然而，已发现了一些具有中抗水平的寄主如干豆和大豆，它们能较全面的控制菌核病。

Carmichaeli leaves were studied after spraying salicylic acid 2 days before inoculation with Septoria aconite Bacc.

用5mmol/L的水杨酸对附子抗病品种和感病品种分别进行诱导，两天后接种乌头壳针孢，研究处理后附子叶片相关酶活性的变化，结果表明，SA诱导后能明显增强附子叶片内POD、PPO、PAL的活性；诱导接种处理比对照接种处理酶活性增加更为明显，同时抗病品种比感病品种酶活性更强，增加幅度更大，高酶活持续时间更长。

Stylosanthes guianensis cv. Mineirao is a variety with high resistance to anthracnose and was to planted widely in Brazil, MSRA gene has been obtained from Mineirao. While its anthrancose resistance mechanism is unknown .

Mineirao柱花草是一种高度抗炭疽病的品种，已在巴西得到了大面积的推广种植，有关研究人员已经对Mineirao柱花草的抗病机理进行了初步研究，从中克隆出MSRA基因，但目前对Mineirao 柱花草抗病机理尚不清楚。

Two complementary dominant genes conditioned the resistance, with a new genetic model, of the maize inbred line were found at adult stage by the genetic analysis based on parents, F 1, F 2 and backcrosses in ...

通过连续两年的经典遗传学研究发现，四一的成株期抗性表现为一种新的抗病遗传模式，该抗性是由两个显性互补抗病基因控制。

Take SYBR Green I as the intercalating fluorescence dye in Real-time PCR,the overexpression of WIPK and SIPK,which were the key genes of MAPK cascades,and the resistant gene PR1 in tobacco treated with copper and magnesium solution were studied.

采用SYBR GreenⅠ作为荧光染料的Real-time PCR方法，明确了铜、镁素能调控烟株体内蛋白激酶级联信号转导途径的关键基因WIPK、SIPK及抗病信号通路末端PR1基因的过量表达，进一步揭示了矿质营养调控烟草对病毒抗病性的分子机制。

The application of MAS in rice bacterial blight resistance and rice blast resistance breeding programs showed it is efficient in selecting individuals containing resistance genes from a crossing population, reducing the scale of subsequent experiment, and breeding variety with pyramided genes.

以抗白叶枯病基因及抗稻热病基因为例，利用分子标志辅助选拔进行杂交后裔族群的筛选，能有效地自杂交后裔族群中筛选出具有抗病基因的个体，并可缩小病原菌接种的试验规模，快速地育成堆叠抗病基因的品系。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。