抗生物素蛋白质

The application of low pressure boiling technique could increase wort boiling temperature to 100.5 ℃ and advance protein agglomeration and reduce solidifiable nitrogen content to 0.5 mg/100 mL. Maillard reaction and Brown reaction were strengthened in the boiling, melanoidin and melanoid compound contents increased, wort chromaticity improved, and the oxidation resistance of wort strengthened. Besides, the boiling intensity dropped from 9 %～12 % to 7 %～8 % and boiling time shortened to 10～20 min, which improved production efficiency and saved 20 % energy and improved beer non-biological stability.

采用低压煮沸工艺，可提高煮沸麦汁温度至100.5 ℃，加强蛋白质凝聚，麦汁可凝固氮下降0.5 mg/100 mL；煮沸过程加强了美拉德反应、"棕色反应"，类黑精、类黑素化合物增加，麦汁色度增加，麦汁的抗氧化能力增强；煮沸强度从9 %～12 %降至7 %～8 %，煮沸时间缩短10～20 min，提高生产效率，节约能源20 %；改善啤酒非生物稳定性。

The application of low pressure boiling technique could increase wort boiling temperature to 100.5 ℃ and advance protein agglomeration and reduce solidifiable nitrogen content to 0.5 mg/100 mL. Maillard reaction and Brown reaction were strengthened in the boiling, melanoidin and melanoid compound contents increased, wort chromaticity improved, and the oxidation resistance of wort strengthened. Besides, the boiling intensity dropped from 9 %～12 % to 7 %～8 % and boiling time shortened to 10～20 min, which improved production efficiency and saved 20 % energy and improved beer non-biological stability.

采用低压煮沸工艺，可提高煮沸麦汁温度至100.5 ℃，加强蛋白质凝聚，麦汁可凝固氮下降0.5 mg/100 mL；煮沸过程加强了美拉德反应、&棕色反应&，类黑精、类黑素化合物增加，麦汁色度增加，麦汁的抗氧化能力增强；煮沸强度从9 %～12 %降至7 %～8 %，煮沸时间缩短10～20 min，提高生产效率，节约能源20 %；改善啤酒非生物稳定性。

Preliminary studies on affinity isolation based on the specific combination between Biotin and Avidin Presently, affinity chromatography is increasing to be a main protein isolation and purification technique for a complicated system because of its high specificity, high rate of recovery, etc.

利用生物素和抗生物素蛋白间的特异性结合作用，将生物素通过化学反应键合到蛋白质的某种官能团上，酶解后的肽段通过固定了抗生物素白蛋白的亲和分离凝胶色谱柱，可以选择性地分离生物素化的肽段，达到简化复杂体系的目的。

Methods ①Human peptides of CYP450 enzymes and their proteinic sequence were analyzed by means of bioinformatics, and certain sequences were chosen and synthesized according to four indexes (Hydrophilicity Plot, Flexible Regions, Antigenic Index and Surface Probability Plot) after homology analysis by software Blast. The synthesized peptides were conjugated to keyhole limpet hemocyanin to increase antigenicity.

①利用生物信息学方法分析CYP1A2、CYP3A4及CYP4A11蛋白的序列，利用DNAstar软件中蛋白质的亲水性结构、柔韧区、抗原指数及表面概率结构指标选择多肽序列，应用Blast软件对所选序列进行同源性分析并合成多肽，与载体蛋白钥孔戚血蓝素偶联，免疫大耳白兔制备抗CYP1A2、3A4及4A11抗体。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。