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Using Colophony-Paraffin embedding tissue section technique and immunohistochemical streptalidin-peroxidase method, we have investigated the distribution of 5-HT neurons in the brain of Camponotus japonicus, compared the immunoreactivity with GABA, and primarily discussed the character of 5-HT distribution.

本实验采用树脂石蜡组织包埋切片技术和链霉菌抗生物素蛋白-过氧化物酶免疫组织化学方法,研究了5-HT能神经元在日本弓背蚁大工蚁脑中的分布情况,并与GABA的分布进行了比较,初步探讨了5-HT在各个脑区的分布规律和特点。

Methods Immunohistochemical streptavidin-peroxidase method was used to detect the expression of MMP-2, MMP-9, TIMP-1, TIMP-2 in 20 cases of normal human cytotrophoblast cells,15 cases of partial hydatidiform moles, 55 cases of complete hydatidiform moles, 15 cases of invasive moles and 8 cases of choriocarcinoma.

应用链霉菌抗生物素蛋白-过氧化物酶免疫组织化学方法对20例正常早孕(2周)绒毛组织、15例部分性葡萄胎、55例完全性葡萄胎、15例侵蚀性葡萄胎、8例绒毛膜癌组织中MMP-2、MMP-9、TIMP-1、TIMP-2进行检测。

The distribution of γ-aminobutyric acid and serotonin (5-HT) immunoreactive neurons in the protocerebrum and deutocerebrum of the butterfly Minathyma schrenckii were studied with the Colophony-paraffin embedding serial section technique and the Streptavidin-peroxidase immunohistochemical method.

采用树脂石蜡(Colophony-Paraffin,CP)组织包埋切片技术和链霉菌抗生物素蛋白-过氧化物酶(Streptavidin-Peroxidase,SP)免疫组织化学方法,首次研究了 GABA 和5-HT 免疫阳性神经元在白斑迷蛱蝶前脑和中脑中的分布。

The structure and 5-HT immunoreactivity in the brain and suboesophageal ganglion of three beetle s, Ambrostoma quadriimpressum, Henosepilachna vigintioctomaculata and Oxycetonia jucunda, were first studied by means of colophony-paraffin embedding serial section technique and strepteavidin-peroxidase immunohistochemical method.

采用树脂石蜡组织包埋连续切片技术,结合免疫组化链霉菌抗生物素蛋白-过氧化物酶染色法,首次对三种甲虫(榆紫叶甲、小青花金龟、马铃薯瓢虫)的脑进行了详细观察研究,分析了三种甲虫脑组织结构特征,初步构建了三种甲虫脑内5-HT抗原系统免疫组化反应模式。

The structure and 5-HT immunoreactivity in the brain and suboesophageal ganglion of three beetles, Ambrostoma quadriimpressum, Henosepilachna vigintioctomaculata and Oxycetonia jucunda, were first studied by means of colophony-paraffin embedding serial section technique and strepteavidin-peroxidase immunohistochemical method. The results showed that the brains of these three taxonomically closely related beetles were remarkably different in composition and size.

采用树脂石蜡组织包埋连续切片技术,结合免疫组化链霉菌抗生物素蛋白-过氧化物酶染色法,首次对三种甲虫(榆紫叶甲、小青花金龟、马铃薯瓢虫)的脑进行了详细观察研究,分析了三种甲虫脑组织结构特征,初步构建了三种甲虫脑内5-HT抗原系统免疫组化反应模式。

A protein found in uncooked egg white that binds to and inactivates biotin.

抗'。''生物素蛋白卵清中发现的一种蛋白质,它可与生物素结合并使生物素''。

Methods Expression of C-erbB2、nm23 and p53 proteins was retros pectively studied by immunohistochemistry, and Kaplan-Meier Method and Cox's proportional hazard regression model were used to analyze the relationship of their expressions with prognosis.

方法应用链霉菌抗生物素蛋白-过氧化酶连接法检测70例卵巢上皮性癌石蜡切片中C-erbB2、nm23和p53蛋白的表达情况。

Methods Expression of C-erbB2、nm23 and p53 proteins was retros pectively studied by immunohistochemistry, and Kaplan-Meier Method and Coxs proportional hazard regression model were used to analyze the relationship of their expressions with prognosis.

方法应用链霉菌抗生物素蛋白-过氧化酶连接法检测70例卵巢上皮性癌石蜡切片中C-erbB2、 nm23和p53蛋白的表达情况。

Methods The ELISA plate was firstly coated with Mycoplasma hyopneumoniae , and then successively incubated with rabbit anti Mycoplasma hyopneumoniae serum and goat anti rabbit IgG biotin to form the solid biotin, which competed with the biotin in the solution for the limited streptavidin horse radish peroxidase conjugate.

酶标板先用猪肺炎支原体包被,再依次用兔抗猪肺炎支原体抗血浆、羊抗兔IgG -生物素温育形成固态生物素,同溶液中的生物素竞争有限量的链霉亲和素 HRP。

In order to study the expression of Gonadotropin-Releasing Hormone in the hypothalamus of estrus milk goat.

为了研究促性腺激素释放激素在发情期奶山羊下丘脑中的表达特点,采用超敏的免疫组化链霉素抗生物素蛋白-过氧化物酶法研究GnRH免疫反应神经元在发情期山羊下丘脑中的分布。

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What would he tell Judith and the children?

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I this is at that time, the opinion with peacockish true girl is full of in a heart.

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