抗生物素

Methods The expression of CD147 and MMP-2 proteins were detected by immunohistochemical method in 46 cases of gliomas, and compared with those in 14 cases of normal brain tissues. The correlation between them was analyzed.

应用免疫组织化学链霉素抗生物素蛋白－过氧化酶连接法，检测46例胶质瘤瘤组织中CD147蛋白及MMP-2蛋白的表达，分别与正常脑组织14例进行对照。

Methods MMP-2 and MMP-9 were examined immunohistochemically in TCCB and 5 normal bladder tissue. The correlation between the expression of MMP-2 and MMP-9 and tumor grade and stage was analysed.

应用链霉菌抗生物素蛋白－过氧化酶免疫组化方法，检测5例正常膀胱组织和49例膀胱移行细胞癌组织中MMP-2和MMP-9的表达水平。

In order to investigate the effect of IFN-γon the expression of TNF-αin the hypothala- mus-pituitary-ovary axisof RU486-induced aborted SD rats, RU486 was used to establish the abortion model, then the aborted rats were administrated with different doses of IFN-γintraperitoneal injection, the method of immunohistochemical ultrasensitive SP was us- ed to study the variation of positive TNF-αcells in hypothalamus, pituitary, ovary and uterus in normal pregnant group A, aborted model group B and three different doses of IFN-γgroup C,D,E. The main goal of this research was to study whether the low dose of IFN-γcould have a protective function to pregnancy and the mechanism of its function so as to provide morph- ologic basis for the scientific using of IFN-γin the process of animal abortion control.

为探讨IFN-γ对流产大鼠下丘脑-垂体-卵巢轴(hypothalamus-pituitary- ovary-axis，HPOA )TNF-α表达的影响，本试验用米非司酮(RU486)建立SD大鼠流产模型，通过腹腔注射不同剂量IFN-γ，采用超敏感免疫组织化学SP法(链霉素抗生物素—过氧化物酶法)，观察了孕13天(d13)SD大鼠正常妊娠A组、流产模型B组、不同剂量IFN-γC、D、E组下丘脑、垂体、卵巢、子宫TNF-α的表达变化，探讨低剂量IFN-γ对妊娠是否有保护作用及其作用机制，期望能为在妊娠过程中如何科学应用IFN-γ防止动物流产提供形态学依据。

Using CP (Colophony-Paraffin) embedding tissue section technique and immunocytochemical method (Streptavidin-Peroxidase, SP), we have investigated the distribution of GABA and 5-HT immunoreactive neurons in the brain of female and male ants, Camponotus Japonicus. Special attention has been paid to the comparative research on the differences between female and male ants. We also try to give possible interpretations leading to these differences in the behavioral aspects.Here we show that in the brain of female and male C.

本实验采用树脂石蜡组织包埋切片技术(国家发明专利ZL98125709.7)和链霉菌抗生物素蛋白-过氧化物酶(Streptavidin-Peroxidase，SP)免疫组织化学方法，对日本弓背蚁雄蚁和雌蚁成虫脑中的GABA和5-HT能神经元的分布进行了详细的研究，比较了雄蚁和雌蚁的异同，并对可能造成这些差异的行为学联系进行了初步的探讨。

Applied rabbit anti-5-HT and mouse monoclonal anti-TPH antisera, the localization of 5-HT and its synthesizing enzyme TPH in the visual system, midbrain, and suboesophageal ganglion of the beetle Harmonia axyridis (Coleoptera: Coccinellidae) was studied by Streptavidin-Peroxidase immunohistochemical method on Colophony-Paraffin embedded serial sections.

运用树脂石蜡组织包埋切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶双染法，采用兔抗5-HT抗血清和鼠抗TPH单克隆抗体，初步构建了异色瓢虫Harmonia axyridis视觉系统、脑中部及咽下神经节5-HT及其合成酶TPH抗原系统的免疫组织化学反应模式。

Applied rabbit anti-5-HT and mouse monoclonal anti-TPH antisera, the localization of5-HT and its synthesizing enzyme TPH in the visual system, midbrain, and suboesophagealganglion of the beetle Harmonia axyridis (Coleoptera: Coccinellidae) was studied byStreptavidin-Peroxidase immunohistochemical method on Colophony-Paraffin embeddedserial sections. An overall observation reveals that the brain structure of the beetle is relativelydistinct.

运用树脂石蜡组织包埋切片技术，结合免疫组化链霉菌抗生物素蛋白-过氧化物酶双染法，采用兔抗5-HT抗血清和鼠抗TPH单克隆抗体，初步构建了异色瓢虫Harmoniaaxyridis视觉系统、脑中部及咽下神经节5-HT及其合成酶TPH抗原系统的免疫组织化学反应模式。

An abundance of avidin in the duct can result in a deficiency of biotin.

导管中抗生物素蛋白过量可导至生物素的缺乏

With this SPR sensor, avidin of concentration of 0.5 nmol/L was examined and a nice response characteristic was obtained. The result indicates that this device could be widely employed for measuring biomolecules.

利用这种传感器，测量浓度为 0.55nmol/L的抗生物素，得到很好的响应特性，证明该装置可用于生物分子的检测。

Methods Avidin biotin-peroxidase complex technique was used to determine mCD14 protein expression in peripheral monocytes in 5 ARDS patients and 10 normal subjects.

对5 例ARDS患者及10 例健康志愿者，分别分离其外周血单核细胞，用抗生物素－生物素－过氧化酶复合物技术检测其mCD14 的表达。

Its principle is as follows:red blood cells are labeled with NHS-Biotin,participated in the circulation and ages in vivo.Using the high affinity between biotin and avidin,the labeled RBCs with specific ages are separated.

其基本原理是用生物素标记一定量的红细胞，使之参与血液循环，并在体衰老，利用biotin与抗生物素蛋白之间的高亲和力，分离带有标记的、具有特定年龄的红细胞。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。