抗狂犬病的

At first, the PRV as antigen was purified by overspeed centrifugal method.

试验所用的第一抗体为兔抗伪狂犬病毒鄂A株的IgG。

A sandwich LAB-ELISA wa s developed to detect Pseudorabies virus on the basis of species-specific monoclonal antibodies in this study.

在伪狂犬病病毒种特异性单克隆抗体的基础上建立了检测该病毒抗原的单抗夹心LAB-ELISA方法。

The purpose of this research is to generateengineering antibody by using phage display technology,and to investigate its binding activitywith the pseudorabies virus.

本研究的目的就是克隆抗伪狂犬病病毒抗体重链、轻链可变区基因，构建单链抗体基因，利用噬菌体展示技术制备噬菌体抗体，并在大肠杆菌中进行可溶性表达，获得具有对伪狂犬病病毒结合活性的表达产物，为以后在治疗、预防上的应用奠定基础。

Neutralizing potency of the patent recombinant anti-rabies virus McAbs, SO57 and SOJB,have been investigated broadly to different rabies virus strains in various animal models.100% Chinese Hamsters could be protected by 100IU/kg SO57 against China prevalent street rabies virus named SBD.We firstly established the mouse model to simulate human postexposure prophylaxis by rabies virus and found SO57 had the same function with HRIG against CVS or three China typical street rabies viruses.However,HRIG could not 100% protect the mice challenged with SBD street virus,rabies virus vaccine alone could not save life from rabies virus infection.The cocktail containing SOJB and SO57(1:1) did not do better than SO57 alone.

用不同的动物模型研究了具有专利的重组人抗狂犬病病毒单克隆抗体SO57、SOJB对不同狂犬病病毒株的中和作用，100IU/kg的SO57能100%保护被中国街毒株SBD攻击的中国仓鼠；首次用小鼠模型模拟人体被狂犬病病毒攻击后的治疗情况，在小鼠被CVS及中国街毒代表株攻击后，SO57与HRIG具有相近的对小鼠的暴露后保护作用；同时结果显示HRIG对SBD株攻击的保护率不能到达100%，仅使用疫苗是不能对感染病毒的小鼠百分之百的保护；SOJB与SO57 1：1联合使用未显示比SO57单独使用更好的保护效果。

Facts as increasing number of dog raising, having not been injected with rabies vaccine and antirabies serum timely or full course after exposure, unstrictly controlling the quality of rabies vaccine as well as lacking good cooperation in different official department on rabies control might serve as factors responsible for the recurrent increase of human rabies.

养犬数增加、没有及时全程使用狂犬病疫苗和抗血清、疫苗质量把关不严以及缺乏不同部门间的合作是流行回升的因素。

Attempts to reduce the economic losseshave also included passive immunization with hyperimmune serum and monoclonal antibodies.

为尽可能减少经济损失，人们也尝试了以抗血清、单克隆抗体对伪狂犬病的被动免疫预防与治疗，并取得一定效果。

BALB/C mice were immunized with the purified PrV of MIN A isolate,mouse spleen cell were fused with myelomas NSo.Culture supernatants of hybridomas were screened by indirect ELISA and immobilization ELISA,positive colonies were cloned three times by limited dilution.

用纯化的伪狂犬病病毒免疫BALB/C小鼠，取脾细胞与NS_0骨髓瘤细胞融合，经间接ELISA和固相化ELISA筛选，3次有限稀释法克隆，获得了1株能稳定分泌抗伪狂犬病病毒单克隆抗体杂交瘤细胞株，命名为OH_6。

The potency of antigens were higher than 6.0 IU/mL. The antigen is suitable immunising horse for producing rabies immunoglobulin.Keywords : Vero cells;Horse serum;Antigen

第5，8天收获病毒滴度可稳定在7.0logLD50/ mL以上，灭活抗原具良好的抗原性，用NIH法测定效价达6.0IU/ mL以上，可用作抗原生产抗狂犬病血清。

In addition, 190 cases of rabies have been bitten for rabies vaccines and anti-rabies serum treatment of pregnant women and 185 infants born alive after one year follow-up, and with rabies has not been bitten by a comparison of babies born to pregnant women, found that spontaneous abortion is the former 4.2%, respectively, and the latter 5.6%, 0.5% pre-placental and 1.6%, 1.6% in diabetic pregnant women and 1.3%, stillbirths 1.6% and 1.5%, 0.5% in children with birth defects and 1.0%, low birth weight 2.6% and 9.9%.

另外，对190例被狂犬咬伤进行狂犬疫苗和抗狂犬病血清治疗的孕妇和185名出生后成活的婴儿进行一年随访，并与未被狂犬咬伤的孕妇所生婴儿进行比较，发现自发性流产分别是前者4.2%和后者5.6%、胎盘前置0.5%和1.6%、孕妇糖尿病1.6%和1.3%、死胎1.6%和1.5%、小儿出生缺陷0.5%和1.0%、低出生体重2.6%和9.9%。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。